Local Redox‐Cycling‐Based Electrochemical Chip Device with Deep Microwells for Evaluation of Embryoid Bodies

Ino, Kosuke; Nishijo, Taku; Arai, Toshiharu; Kanno, Yusuke; Takahashi, Yasufumi; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1002/anie.201201602

Cited by 75 publications

(122 citation statements)

References 13 publications

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“…The development of several types of electrochemical devices for bioassay applications, including analyses of yeast and mammalian cells, has been reported. [29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44] IDA electrodes were incorporated to amplify signals from cells and provide highly sensitive assays. Yeast cellbased IDA electrodes were utilized for the detection of hormone active chemicals.…”

Section: Chip-based Devices For Electrochemical Bioanalysismentioning

confidence: 99%

“…To overcome this difficulty, we proposed a novel electrochemical approach based on redox cycling, the local redox cycling-based electrochemical (LRC-EC) system. [33][34][35][36][37][38][39][40][41][42][43][44] In the LRC-EC system, two arrays of electrodes (n rows and n columns of electrodes) are orthogonally placed to produce n 2 crossing points (Fig. 4).…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

“…To address these problems, planar comb-type IDA electrodes were incorporated into the LRC-EC device, which allowed for all electrodes to be arranged on a single substrate. [34][35][36][37][38][39][40] One of the LRC-EC devices has 1,024 sensors/41 mm 2 , 39 making the device well-suited for high-throughput amperometric detection and rapid electrochemical imaging. In addition, vertically separated electrodes were incorporated into the LRC-EC device to further increase the sensor density.…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

“…5). 35,38 Therefore, the proposed LRC-EC system is useful for cell screening in tissue engineering and is ideal for high-throughput biosample assays and imaging applications.…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

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Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

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Here, we present an overview of our recent research progress in development of electrochemical devices/systems for bioassays. These devices/systems are based on microchemistry and micro-electromechanical systems (MEMS) for sophisticated analytical and electrochemical measurements. In particular, we exploit the unique chemical reactions occurring in micrometer-size spaces and/or involving micrometer-size structures for bioassays, including cell analyses. This paper addresses five topics: probe-, chip-, large-scale-integration chip-, and dielectrophoresisbased devices, and electrodeposition of hydrogels for bioassays.

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Section: Chip-based Devices For Electrochemical Bioanalysismentioning

confidence: 99%

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

“…5). 35,38 Therefore, the proposed LRC-EC system is useful for cell screening in tissue engineering and is ideal for high-throughput biosample assays and imaging applications.…”

Section: Electrochemical Imaging and High-throughput Analysismentioning

confidence: 99%

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Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Ino

2015

Electrochemistry

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“…Redox cycling is an electrochemical method consisting of two closely spaced and individually addressable electrodes to amplify electrochemical signals. 4,5 Target molecules reacting at one electrode (the generator electrode) are recovered at the other electrode (the collector electrode). The recovered molecules react again at the generator electrode, thus amplifying the current signals.…”

Section: Introductionmentioning

confidence: 99%

Redox Cycling-based Electrochemical Reporter Gene Assay for Single Cells Using a Scanning Electrochemical Microscope-microwell System

et al. 2016

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Single-cell analyses are important for providing new insights into cellular biology. Here we report an electrochemical reporter gene assay for single cells using a scanning electrochemical microscope (SECM)-microwell system. Each microwell trapped a single cell that synthesized a reporter protein, secreted alkaline phosphatase (SEAP). The SEAP catalyzed the hydrolysis of p-aminophenyl phosphate to p-aminophenol (PAP). A disk electrode in the SECM was positioned above the microwell and monitored the oxidation currents of PAP derived from SEAP. In addition, ring electrodes were prepared on the microwell device to induce redox cycling between the ring and disk electrodes, thus amplifying the electrochemical signals from the reporter protein. The redox cycling-based electrochemical reporter gene assay is useful for single-cell analyses.

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Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

et al. 2017

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A rapid fabrication method of microgap electrodes using inkjet printing is described. In this approach, the lateral spacing between two printed electrode lines is precisely controlled down to 1 µm without any surface modification or substrate patterning. The strong confinement, well below typical resolution of inkjet printing, relies on complete solvent evaporation between the printing of adjacent electrode structures, which is achieved by controlling the printing speed and temperature profiles. The feasibility of this method is demonstrated by writing electrode structures with two distinct inks, based on carbon and silver nanoparticles, with comparable results. As an application proof-of-principle, arrays of microgap electrodes are fabricated using a carbon nanoparticle ink for electrochemical detection based on redox-cycling, a technique in which the sensitivity of the device depends on the distance between the two electrodes. The redox-cycling amplification of electrochemical signals is demonstrated and it is shown that the printed microgap device can be used as an electrochemical biosensor for the determination of human immunodeficiency virus (HIV)-related single-stranded DNA. This work presents a promising new approach for fabricating low-cost and label-free redox-cycling biosensors using all-inkjet-printed electrodes. www.adv-biosys.com

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Local Redox‐Cycling‐Based Electrochemical Chip Device with Deep Microwells for Evaluation of Embryoid Bodies

Cited by 75 publications

References 13 publications

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Microchemistry- and MEMS-based Integrated Electrochemical Devices for Bioassay Applications

Redox Cycling-based Electrochemical Reporter Gene Assay for Single Cells Using a Scanning Electrochemical Microscope-microwell System

Flexible Microgap Electrodes by Direct Inkjet Printing for Biosensing Application

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