Local release of magnesium from mesoporous TiO 2 coatings stimulates the peri-implant expression of osteogenic markers and improves osteoconductivity in vivo

Galli, S.; Naito, Yoshihito; Karlsson, Johan; He, Wenwu; Miyamoto, Ikuya; Xue, Ying; Andersson, Martin; Mustafa, Kamal; Wennerberg, Ann; Jimbo, Ryo

doi:10.1016/j.actbio.2014.08.011

Cited by 64 publications

(63 citation statements)

References 34 publications

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“…In previous studies, we have documented the osteoconductive and osteogenic potential of magnesium when released from mesoporous titania surfaces and confirmed the associated increase of bone formation and biomechanical anchorage [21–23]. …”

Section: Introductionsupporting

confidence: 60%

“…In previous investigations, the effects of the local administration of Mg ions from mesoporous coatings on the bone anchorage of titanium implants were explored and positive outcomes in healthy bone were noted [22, 23]. Magnesium-doped titanium implants required higher reverse torque strength in order for them to be removed compared to non-doped controls.…”

Section: Discussionmentioning

confidence: 99%

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The effect of magnesium on early osseointegration in osteoporotic bone: a histological and gene expression investigation

et al. 2017

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SummaryMagnesium has a key role in osteoporosis and could enhance implant osseointegration in osteoporotic patients. Titanium implants impregnated with Mg ions were installed in the tibia of ovariectomized rats. The release of Mg induced a significant increase of bone formation and the expression of anabolic markers in the peri-implant bone.IntroductionThe success of endosseous implants is highly predictable in patients possessing normal bone status, but it may be impaired in patients with osteoporosis. Thus, the application of strategies that adjuvate implant healing in compromized sites is of great interest. Magnesium has a key role in osteoporosis prevention and it is an interesting candidate for this purpose. In this study, the cellular and molecular effects of magnesium release from implants were investigated at the early healing stages of implant integration.MethodsOsteoporosis was induced in 24 female rats by means of ovariectomy and low-calcium diet. Titanium mini-screws were coated with mesoporous titania films and were loaded with magnesium (test group) or left as native (control group). The implants were inserted in the tibia and femur of the rats. One, 2 and 7 days after implantation, the implants were retrieved and histologically examined. In addition, expression of genes was evaluated in the peri-implant bone tissue at day 7 by means of quantitative polymerase chain reactions with pathway-oriented arrays.ResultsThe histological evaluation revealed that new bone formation started already during the first week of healing for both groups. However, around the test implants, new bone was significantly more abundant and spread along a larger surface of the implants. In addition, the release of magnesium induced a significantly higher expression of BMP6.ConclusionsThese results provide evidence that the release of magnesium promoted rapid bone formation and the activation of osteogenic signals in the vicinity of implants placed in osteoporotic bone.Electronic supplementary materialThe online version of this article (doi:10.1007/s00198-017-4004-5) contains supplementary material, which is available to authorized users.

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Section: Introductionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

The effect of magnesium on early osseointegration in osteoporotic bone: a histological and gene expression investigation

et al. 2017

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“…HA coatings enriched with magnesium on titanium implants promoted early osseointegration after 2 weeks compared to pure HA coatings [23]. Mg enrichment of TiO 2 implant coatings caused increased gene expression of osteogenic differentiation markers in surrounding bone [24]. However, the effect of Mg In this study, bioplotted PCL scaffolds were coated with CaP and CaP doped with Mg (CaPþMg).…”

Section: Introductionmentioning

confidence: 93%

Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping

et al. 2015

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“…Specifically, Ca 2+ is of assistance in the G1 phase (increase in cell size by formation of proteins and m-RNA), whereas Mg 2+ helps in accelerating the G1 to S phase transfer, thereby promoting cell growth. Li 1+ also acts as a catalyst in the G1 phase to form proteins and m-RNA (provided its concentration is within the tolerance limit) [18,19].…”

Section: Microstructure-biodegradationcytocompatibility Relationship mentioning

confidence: 99%

Towards microstructure-cytocompatibility relationship in ultralight Mg-4Li-1Ca (LX41) alloy for degradable implant applications

et al. 2016

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Cytocompatibility and biodegradation behaviour were investigated for a newly developed ultralight Mg-4Li-1Ca (LX41) alloy with different starting microstructures. This established the important role of microstructure in determining the pH variation, H 2 evolution and amount of ions (Mg, Li, Ca) released during degradation. Thus, different thermomechanical treatments were utilised to alter the microstructure of the alloy and to evaluate its bio-response. The as-cast and two step rolled+annealed (TA30) materials showed stronger X-ray diffraction peaks for Mg-and Li-containing hydroxyapatite (HA) than the two step rolled (TSR) and the equal channel angular pressed (ECAP) materials. It was also found that the as-cast and TA30 variants of LX41 showed good cell viability of more than 100% with preferential attachment and healthier cell morphology than the TSR material. This is associated with greater content of Mg and Li ions released during alloy degradation. These act as mediators to establish contact between the cells and the substrate, and thereby promote cell attachment and proliferation. These findings suggest the existence of a strong microstructure-cytocompatibility relation for the present LX41 alloy, which can be used as an important tool for further magnesium alloy development for biological applications.

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Local release of magnesium from mesoporous TiO 2 coatings stimulates the peri-implant expression of osteogenic markers and improves osteoconductivity in vivo

Cited by 64 publications

References 34 publications

The effect of magnesium on early osseointegration in osteoporotic bone: a histological and gene expression investigation

The effect of magnesium on early osseointegration in osteoporotic bone: a histological and gene expression investigation

Pulsed laser deposition of magnesium-doped calcium phosphate coatings on porous polycaprolactone scaffolds produced by rapid prototyping

Towards microstructure-cytocompatibility relationship in ultralight Mg-4Li-1Ca (LX41) alloy for degradable implant applications

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