2021
DOI: 10.1007/s00705-021-05301-w
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Local spread of influenza A (H1N1) viruses without a mutation for the maximum duration of an epidemic season in Japan

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Cited by 2 publications
(1 citation statement)
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“…Total RNA was extracted using a QIAamp Viral RNA Mini Kit (QIAGEN, Tokyo, Japan). Eight RNA segments of in uenza A virus were simultaneously reverse transcribed and ampli ed using a SuperScript™III One Step RT-Polymerase Chain Reaction (PCR) System (Life Technologies, Tokyo, Japan) with the MBTuni-12/MBTuni-13 primer pair [5,20]. The rst-round PCR products were subjected to a second PCR to amplify NS1 using A-NS-M13 primers (sense: 5-TGTAAAACGACGGCCAGTAGCAAAAGCAGGGTGACAAAGACA-3; antisense: 5-CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTTTTAT-3).…”
Section: Ns1 Sequencingmentioning
confidence: 99%
“…Total RNA was extracted using a QIAamp Viral RNA Mini Kit (QIAGEN, Tokyo, Japan). Eight RNA segments of in uenza A virus were simultaneously reverse transcribed and ampli ed using a SuperScript™III One Step RT-Polymerase Chain Reaction (PCR) System (Life Technologies, Tokyo, Japan) with the MBTuni-12/MBTuni-13 primer pair [5,20]. The rst-round PCR products were subjected to a second PCR to amplify NS1 using A-NS-M13 primers (sense: 5-TGTAAAACGACGGCCAGTAGCAAAAGCAGGGTGACAAAGACA-3; antisense: 5-CAGGAAACAGCTATGACCAGTAGAAACAAGGGTGTTTTTTAT-3).…”
Section: Ns1 Sequencingmentioning
confidence: 99%