Local Transient Myocardial Liposomal Gene Transfer of Inducible Nitric Oxide Synthase Does Not Aggravate Myocardial Function and Fibrosis and Leads to Moderate Neovascularization in Chronic Myocardial Ischemia in Pigs

Abegunewardene, Nico; Schmidt, Kai‐Helge; Vosseler, Markus; Dreher, Michael; Keller, Tandis; Hoffmann, Nico; Veit, Kerstin; Petersen, Steffen E.; Lehr, Hans‐Anton; Schreiber, Laura M.; Gori, Tommaso; Horstick, Georg; Münzel, Thomas

doi:10.1111/j.1549-8719.2010.00002.x

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…The responses of all the cultivars under study to PEG-induced drought stress in a hydroponic culture, and drought stress treatment in a pot experiment have been summarized in Supplementary Tables S2 and S3. Previous reports suggest that drought stress affects seed germination, seedling growth, and other physiological parameters [39,60,61]. Root development is significant for plants during adaptation to drought stress since water and nutrients are acquired from the soil or any other nutrient solution in the case of plants grown in a hydroponic medium [62].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Iraqi Rice Cultivars for Their Tolerance to Drought Stress

et al. 2020

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Drought stress is a serious problem around the globe and particularly in the Republic of Iraq. Rice is the third most consumed crop for the Iraqi people; however, its cultivation and production is very low due to several challenges including drought. The current study was performed to evaluate five Iraqi rice cultivars along with relevant (drought-tolerant and drought-susceptible) controls under drought stress, either by treatment with 10% PEG (polyethylene glycol) or through water withholding to induce natural drought stress. The phenotypes of all the cultivars were evaluated and the transcriptional responses of key drought-responsive candidate genes, identified through the EST-SSR marker-based approach, were studied. We also studied transcript accumulation of drought-related transcriptional factors, such as OsGRASS23, OsbZIP12, and OsDREB2A. Moreover, the reference cultivars also included a drought-tolerant inter-specific cultivar Nerica 7 (a cross between Oryza sativa ssp. indica X O.glaberrima). Among the cultivars, the more drought-tolerant phenotypic characteristics and higher transcript accumulation of drought-related marker genes OsE647 and OsE1899 and transcriptional factors OsGRASS23, OsbZIP12, and OsDREB2A were observed in four (out of five) significantly drought-tolerant Iraqi cultivars; Mashkab, followed by Furat, Yasmen, and Amber 33. On another note, Amber Barka was found to be significantly drought susceptible. Mashkab and Amber Barka were found to be the most drought-tolerant and-susceptible cultivars, respectively. The identified tolerant cultivars may potentially serve as a genetic source for the incorporation of drought-tolerant phenotypes in rice.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Iraqi Rice Cultivars for Their Tolerance to Drought Stress

et al. 2020

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“…12 Most studies succeeded in presenting improved cardiac function with improved perfusion. Other genes delivered to promote angiogenesis in large animals include CD151, 66 inducible nitric oxide synthase, 18 Angiopoietin-1 (ref. 53) and hypoxia inducible factor-1a.…”

Section: Angiogenesismentioning

confidence: 99%

“…In some studies, DNA complexed with liposome was employed to improve the transfection efficiency. 18,91,93 Viral vectors Adenovirus has been the most frequently employed viral vector in large animal cardiac gene delivery studies. This vector efficiently transduces both dividing and non-dividing cells with significantly higher efficiency than plasmid DNA.…”

Section: Non-viral Vectorsmentioning

confidence: 99%