Local Yield and Compliance in Active Cell Monolayers

Hopkins, Austin; Chiang, Michael; Loewe, Benjamin; Marenduzzo, Davide

doi:10.1103/physrevlett.129.148101

Cited by 19 publications

(13 citation statements)

References 65 publications

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“…To connect these mechanical properties to cellular processes, it is essential to have access at the same time to the cell structure and to its dynamics (e.g. cell shapes or rearrangements) (14).…”

Section: Motivation and State Of The Artmentioning

confidence: 99%

A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization

2022

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Mechanical constraints have a high impact on development processes, and there is a need for new tools to investigate the role of mechanosensitive pathways in tissue reorganization during development. We present here experiments where embryonic cell aggregates are aspired through constrictions in microfluidic channels, generating highly heterogeneous flows and high cell deformations that can be imaged using two-photon microscopy. This approach provides a way to measure in situ local viscoelastic properties of 3D tissues and connect them to intracellular and intercellular events such as cell shape changes and cell rearrangements. Perspectives include applications on organoids to investigate and quantify rheological properties of tissues, and to understand how constraints affect development.

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Section: Motivation and State Of The Artmentioning

confidence: 99%

A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization

2022

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“…On the other hand, application of a vertex model to het- erogeneous cell layers [30] and experiments on primary tumour explants [31] show rigidity percolation based on edge tension network gives rise to finite shear modulus in tumor explants consisting of heterogeneous mixture of soft and stiff cancer cells. Notwithstanding these seminal and important contributions, the universality of the transition between active solid (glass-like) and fluid phases in cellular systems and its broader applicability is yet to be established [7,29,[32][33][34][35][36][37]. Therefore, despite the immense significance of transition from glassy-to fluid-like state in cell collectives in various biological processes, the nature of transition remains elusive.…”

mentioning

confidence: 99%

Stress percolation criticality of glass to fluid transition in active cell layers

Monfared¹,

Ravichandran²,

Andrade³

et al. 2022

Preprint

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“…Our approach also avoids issues with orientational anisotropy associated with lattice models like the Cellular Potts Model [45]. In principle, phase field approaches [20,[55][56][57][58][59] would also avoid lattice artifacts, but our scale of ∼ 3000 cells is an order of magnitude larger than typical applications of even simplified phase-field models [60][61][62]. Earlier papers have modeled elongated self-propelled objects with particle-field and/or Gay-Berne approaches [63,64], though without explicitly describing deformability.…”

Section: Broader Modeling Considerationsmentioning

confidence: 99%

Migration and division in cell monolayers on substrates with topological defects

Kaiyrbekov

Endresen

Sullivan

et al. 2022

Preprint

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Collective movement and organization of cell monolayers are important for wound healing and tissue development. Recent experiments highlighted the importance of liquid crystal order within these layers, suggesting that +1 topological defects have a role in organizing tissue morphogenesis. We study fibroblast organization, motion and proliferation on a substrate with micron-sized ridges that induce +1 and -1 topological defects using simulation and experiment. We model cells as self-propelled deformable ellipses that interact via a Gay-Berne potential. Unlike earlier work on other cell types, we see that density variation near defects is not explained by collective migration. We propose instead that fibroblasts have different division rates depending on their area and aspect ratio. This model captures key features of our previous experiments: the alignment quality worsens at high cell density and, at the center of the +1 defects, cells can adopt either highly anisotropic or primarily isotropic morphologies. Experiments performed with different ridge heights confirm a new prediction of this model: suppressing migration across ridges promotes higher cell density at the +1 defect. Our work enables new mechanisms for tissue patterning using topological defects.

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Local Yield and Compliance in Active Cell Monolayers

Cited by 19 publications

References 65 publications

A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization

A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization

Stress percolation criticality of glass to fluid transition in active cell layers

Migration and division in cell monolayers on substrates with topological defects

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