Localisation cytologique du cuivre et de quelques autres métaux dans la glande digestive de la seiche, <i>Sepia officinalis</i> L (Mollusque Céphalopode)

Martoja, M.; Marcaillou, Christiane

doi:10.1139/f93-063

Cited by 16 publications

(20 citation statements)

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“…These granules function in the storage and excretion of essential and non-essential metals, and their production is common in all major phyla (Brown 1982, George 1982. Thus, the observed increase of total cadmium levels in the digestive gland of cephalopods, resulting in the storage of cadmium in an insoluble form rather than via binding to cytosolic proteins, could be considered as more (Martoja & Marcaillou 1993). These authors suggested that the metals would be complexed by metallothioneins in these spherules.…”

Section: Discussionmentioning

confidence: 99%

Cadmium detoxification processes in the digestive gland of cephalopods in relation to accumulated cadmium concentrations

Bustamante

Cosson

Gallien

et al. 2002

Marine Environmental Research

150

113

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The high concentrations of cadmium recorded in the digestive gland of cephalopods from various temperate and subpolar waters suggest that these molluscs have developed efficient cadmium detoxification mechanisms. The subcellular distribution of cadmium in the digestive gland cells was investigated in seven cephalopod species from the Bay of Biscay (France) and the Faroe Islands. In most species, cadmium was mainly found in the cytosolic fraction of the digestive gland cells, reaching up to 86% of the total cadmium for the squid Loligo vulgaris from the Bay of Biscay. But species with the highest total level of cadmium showed a higher percentage of cadmium associated to insoluble compounds. The quantification of metallothioneins (MTs) by the polarographic method was performed in order to evaluate the involvement of these proteins in the detoxification of the high amounts of bioaccumulated cadmium. Metallothionein levels in cephalopods ranged form 742 +/- 270 to 3478 +/- 1572 microg/g wet weight. No relationship could be established between total cadmium, cytosolic cadmium and MT levels suggesting the occurrence of other Cd-binding ligands. Although these proteins have not been characterised, as cadmium in the digestive gland of cephalopods is mainly associated with soluble ligands, a high potential transfer to predators can be predicted.

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Section: Discussionmentioning

confidence: 99%

Cadmium detoxification processes in the digestive gland of cephalopods in relation to accumulated cadmium concentrations

Bustamante

Cosson

Gallien

et al. 2002

Marine Environmental Research

150

113

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“…The occurrence of sulphydryl-rich proteins such as metallothioneins have been found in the cephalopod digestive gland (Tanaka et al 1983, Bustamante et al 2002b. Histochemical and microanalytical investigations in the digestive gland of the cuttlefish Sepia officinalis have shown that Ag is associated with Cu and Zn in spherules accumulated in the basal cells, and these spherules are composed of metallothionein-like proteins (Martoja & Marcaillou 1993). Metalloproteins play a major role in the homeostasis of Cu and Zn and are well known for their capacity to bind Ag, Cd and Hg (see e.g.…”

Section: Comentioning

confidence: 99%

Uptake, transfer and distribution of silver and cobalt in tissues of the common cuttlefish Sepia officinalis at different stages of its life cycle

Bustamante¹,

Teyssié

Danis

et al. 2004

Mar. Ecol. Prog. Ser.

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Three pathways of exposure (sediment, seawater and food) were examined to determine transfer of 110m Ag and 57 Co in juvenile cuttlefish Sepia officinalis. Additional experiments were conducted on adult cuttlefish and their eggs/embryos in order to assess bioaccumulation patterns at different stages of the organism's life cycle. Eggs, juveniles and adults readily accumulated both Ag and Co from seawater. In eggs, both metals were predominantly adsorbed onto the capsule membrane (≥ 60% for Ag and ≥ 99% for Co), indicating that the latter may act as an effective shield to limit exposure of embryos to soluble metals. Adult cuttlefish incorporated waterborne radiotracers mainly in their muscular tissues (≥ 60% of the whole-body burden); subsequent metal retention was greater for Co (biological half-life, T b ! = 34 d) than for Ag (T b ! = 7 d). Turnover of Co ingested with food was much more rapid in juveniles (T b ! = 5 d) than in adults (T b ! = 990 d), suggesting that the functional maturation of the digestive gland was not complete in the juveniles. With ingested Ag, retention was roughly similar for juveniles and adults (T b ! = 13 and 9 d, respectively). Transfer from sediments was negligible for Co and Ag. Regardless of the exposure pathway, the digestive gland of juveniles and adults contained the major fraction of incorporated metal either following uptake or after depuration. This observation demonstrates that substantial metal transfer takes place from several organs to the digestive gland, and further highlights the major role this organ plays in metal storage and detoxification processes in these cephalopods. KEY WORDS: Metal · Radiotracer · Bioaccumulation · Digestive gland · CephalopodsResale or republication not permitted without written consent of the publisher

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“…These "phosphate granules" are easily observed with the light microscope and, interestingly, the presence of calcium has been demonstrated histochemically in tissue sections stained with the cobalt method (Marig6mez et al, 1986). On the other hand, calcium cells (Schoettli & Seiler, 1970;Marig6mez et al, 1986;Recio et al, 1988), and basal cells of cephalopods (Martoja & Marcaillou, 1993), which are also equivalent to basophilic cells (Marig6mez et al, 1995), can accumulate metals such as copper and zinc. In addition, metals other than calcium have also been localized in the phosphate granules of basophilic cells (Mason et al, 1984;Nott & Langston, 1989;Marig6mez et al 1990a).…”

Section: Discussionmentioning

confidence: 99%

Autometallographic localization of protein-bound copper and zinc in the common winkle,Littorina littorea: A light microscopical study

Soto¹,

Cajaraville²,

Angulo³

et al. 1996

Histochem J

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Copper (Cu), zinc (Zn) and calcium (Ca) were demonstrated histochemically by means of conventional stains (rubeanic acid for copper, dithizone for zinc, and cobalt nitrate for calcium) and by autometallography in various tissues of winkles (Littorina littorea) sublethally exposed to either copper or zinc dissolved in sea water. Rubeanic acid and dithizone procedures exhibited poor sensitivity: there was no positive reaction after fixation tissues with Bouin's fixative, and only a weak reaction after ethanol fixation. Autometallography, however, produced a positive reaction with both fixatives in the form of black silver deposits in some key cell types. In winkles not exposed to either copper nor zinc, autometallographically demonstrated metals were found in the connective tissue pore cells, the lysosomes of digestive cells, the basal lamina of the digestive tubule epithelium, and cytoplasmic granules in the epithelial cells of the stomach wall. In addition, in winkles exposed to copper, metal deposits were present in some apical cytoplasmic granules of ciliated cells in the gill epithelium, the mucous secretion of gill mucocytes, and the circulating haemocytes. In winkles exposed to zinc, metal deposits were found in the basal cytoplasmic granules of ciliated cells in the gill epithelium, the mucous secretion of gill mucocytes, the apex and basal lamina of the nephrocytes in the kidney, and the connective tissue layer surrounding the blood vessels. Additionally, calcium was demonstrated histochemically in the cytoplasm of digestive cells, the cytoplasm of the epithelial cells of the stomach wall, the mucocytes of gills, the basal lamina of the kidneys, the haemocytes, the calcium and pore cells of connective tissue, and the oocyte cytoplasm. Metals were not detected by any procedure in sperm cells, in the cytoplasmic granules of oocytes, or in the basophilic cells in the digestive tubules. In conclusion, autometallography is a highly sensitive method and provides an excellent tool to localize protein-bound copper and zinc in molluscan tissues, and its use in combination with conventional histochemical or chemical methods is highly recommended.

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Localisation cytologique du cuivre et de quelques autres métaux dans la glande digestive de la seiche, Sepia officinalis L (Mollusque Céphalopode)

Cited by 16 publications

References 0 publications

Cadmium detoxification processes in the digestive gland of cephalopods in relation to accumulated cadmium concentrations

Cadmium detoxification processes in the digestive gland of cephalopods in relation to accumulated cadmium concentrations

Uptake, transfer and distribution of silver and cobalt in tissues of the common cuttlefish Sepia officinalis at different stages of its life cycle

Autometallographic localization of protein-bound copper and zinc in the common winkle,Littorina littorea: A light microscopical study

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