Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding

Rodríguez, V Minerva García; Llambí, Silvia; Postiglioni, A.; Guevara, Karina; Rincón, Gonzalo; Fernández, Gabriel; Mernies, B.; Arruga, M.V.

doi:10.1186/1297-9686-34-6-649

Cited by 18 publications

(11 citation statements)

References 11 publications

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“…FS-inducing agents include mutagenic and clastogenic chemicals [58]. Fragile sites must be induced with chemical compounds because the spontaneous expression of fragile sites common on metaphase plates is very low (less than 5%), which makes them very difficult to detect [59]. Fragile sites are a natural element in the chromosome structure of all species and show genetic conservatism in the evolution of mammals [60].…”

Section: Discussionmentioning

confidence: 99%

Assessment of genome stability in various breeds of cattle

Wójcik

Szostek

2019

PLoS ONE

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Chromosomal instability is a type of genome instability involving changes in genetic information at the chromosomal level. The basic tests used to identify this form of instability are sister chromatid exchange (SCE) tests and identification of fragile sites (FS). SCE is the process by which sister chromatids become fragmented as a result of DNA strand breakage and reassembly, followed by exchange of these fragments. FS can be observed in the form of breaks, gaps or constrictions on chromosomes, which often result from multiple nucleotide repeats in DNA that are difficult to replicate. The research material was the peripheral blood of ten breeds of cattle raised in Poland, including four native breeds covered by a genetic resources conservation programme, i.e. Polish Red, Polish Red-and-White, White-Backed, and Polish Black-and-White, as well as Polish Holstein-Friesian, Simmental, Montbéliarde, Jersey, Limousine and Danish Red. Two tests were performed on chromosomes obtained from in vitro cultures: SCE and FS. The average frequency of SCE was 5.08 ± 1.31, while the incidence of FS was 3.45 ± 0.94. Differences in the incidence of SCE and FS were observed between breeds. The least damage was observed in the Polish Red and White-Backed breeds, and the most in Polish Holstein-Friesians. The most damage was observed in the interstitial part of the chromosomes. Age was shown to significantly affect the incidence of SCE and FS. Younger cows showed less damage than older ones (SCE: 4.84 ± 1.25; 5.34 ± 1.24; FS: 3.10 ± 0.88, 3.80 ± 0.92).

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Section: Discussionmentioning

confidence: 99%

Assessment of genome stability in various breeds of cattle

Wójcik

Szostek

2019

PLoS ONE

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“…Various frequencies of damage within a species have been observed by researchers analysing karyotypes of various breeds of animals (Di Berardino et al 1983;Iannuzzi et al 1991;Riggs et al 1993;Ronne 1995;Di Meo et al 2000;Rodriguez et al 2002;Danielak-Czech and Słota 2004;Peretti et al 2007Peretti et al , 2008Nicodemo et al 2008;Albarella et al 2009;Wójcik et al 2011Wójcik et al , 2012. Differences between mean numbers of various forms of damages in genetic material result from the fact that their frequency is influenced by the breed.…”

Section: Discussionmentioning

confidence: 99%

Analysis of chromatin instability of somatic cells in sheep

et al. 2018

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Cytogenetic tests are highly reliable, sensitive indicators of the early effects of genomic instability. Their results provide information on the organism’s susceptibility to exogenous and endogenous factors and are measures of the degree of repair of DNA damage. Our study assessed spontaneously occurring damage in following four breeds of sheep: Polish Heath, Polish Lowland (Zelazna variety), Polish Blackhead, and Berrichon du Cher. Instability was identified using the following three different tests: a sister chromatid exchange (SCE) assay, identification of fragile sites, and the comet assay. The distribution of instabilities varied depending on the breed. The mean frequency of SCEs was 5.13 ± 1.58, whereas that of fragile sites was 3.30 ± 1.24. The mean level of DNA damage (% head DNA) was 96.52 ± 6.59. The most damage to genetic material was observed in the Berrichon du Cher sheep, and the least in the Polish Heath sheep. The tests used are reliable biomarkers of genome stability in animal breeds, as well as in individuals within breeds.

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“…1996). In cattle, various fragile sites were identified (Rodriguez et al. 2002); thus, we must consider the eventuality that these sites are more frequently involved in RCP formation.…”

Section: Discussionmentioning

confidence: 99%

“…In humans, chromosomes carrying fragile site show more inclination to be involved in RCP than expected (Cohen et al 1996). In cattle, various fragile sites were identified (Rodriguez et al 2002); thus, we must consider the eventuality that these sites are more frequently involved in RCP formation. Moreover, if we presume the existence of an RCP deriving from two fragile site breakpoints, the likelihood of being visible is very low.…”

Section: Discussionmentioning

confidence: 99%

Reciprocal translocations in cattle: frequency estimation

Lorenzi

Morando

Planas

et al. 2012

J Animal Breeding Genetics

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SummaryChromosomal anomalies, like Robertsonian and reciprocal translocations represent a big problem in cattle breeding as their presence induces, in the carrier subjects, a well documented fertility reduction. In cattle reciprocal translocations (RCPs, a chromosome abnormality caused by an exchange of material between nonhomologous chromosomes) are considered rare as to date only 19 reciprocal translocations have been described. In cattle it is common knowledge that the Robertsonian translocations represent the most common cytogenetic anomalies, and this is probably due to the existence of the endemic 1;29 Robertsonian translocation. However, these considerations are based on data obtained using techniques that are unable to identify all reciprocal translocations and thus their frequency is clearly underestimated.The purpose of this work is to provide a first realistic estimate of the impact of RCPs in the cattle population studied, trying to eliminate the factors which have caused an underestimation of their frequency so far. We performed this work using a mathematical as well as a simulation approach and, as biological data, we considered the cytogenetic results obtained in the last 15 years. The results obtained show that only 16% of reciprocal translocations can be detected using simple Giemsa techniques and consequently they could be present in no less than 0,14% of cattle subjects, a frequency five times higher than that shown by de novo Robertsonian translocations. This data is useful to open a debate about the need to introduce a more efficient method to identify RCP in cattle.-3 -

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Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding

Cited by 18 publications

References 11 publications

Assessment of genome stability in various breeds of cattle

Assessment of genome stability in various breeds of cattle

Analysis of chromatin instability of somatic cells in sheep

Reciprocal translocations in cattle: frequency estimation

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