Localisation of human osteosarcoma by antitumour monoclonal antibody

Abstract

Immunoscintigraphy using radioisotope-labelled monoclonal antibody prepared against osteosarcoma 791T cells was used to detect a primary osteosarcoma. The eight-centimetre tumour was detected using rectilinear scintigraphy of 131I-labelled antibodies. Image enhancement was achieved by subtraction of blood-pool radioactivity labelled with technetium-99m. The ratio of tumour to non-tumour uptake of radioactivity (5:1) suggested that antibody targeting of therapeutic agents is feasible.

Localization of anti-osteogenic sarcoma monoclonal antibody 791T/36 in a primary human osteogenic sarcoma and its subsequent xenograft in immunodeprived mice

“…Initially it was shown that antibody localized in a primary osteogenic sarcoma in one patient [6]. In that case, however, localization was confirmed only by gamma-camera imaging of 1311-791T/36 accumulation at the tumour site.…”

“…The anti-human osteogenic sarcoma monoclonal antibody 791T/36 labelled with 13iI was previously reported to localize in a primary human osteogenic sarcoma [6]. In the present study, excised turnout from a further osteogenic sarcoma patient undergoing successful imaging with 131I-labelled antibody has been used to establish a xenograft in immunodeprived mice.…”

“…Although this is the basis for clinical tumour-imaging trials with antibodies labelled with appropriate radionuclides [1,6,16,17], there are few or no studies on imaging of an individual human tumour initially in situ and with subsequent confirmation of the localization with the tumour as a xenograft in immunodeprived hosts.…”

Immune responses to the 105AD7 human anti-idiotypic vaccine after intensive chemotherapy, for osteosarcoma

“…This may have been due to the immunosuppressive effects of large tumour burden as a neoadjuvant trial showed promising results indicating a valuable immune response (Durrant et al, 2000a, b;Schwann et al, 2000). As 791T/36 was originally raised against a human osteogenic tumour cell line and was used successfully to image osteosarcomas in vivo (Farrands et al, 1983), we proposed that 105AD7 may be a suitable vaccine for treatment of this disease. Previous experience with colorectal cancer suggested that 105AD7 would be most effective in treatment of patients with minimal residual disease.…”

Immunolocalization of the murine monoclonal antibody, 791T/36 within primary human colorectal carcinomas and identification of the target antigen

“…Investigations have been completed upon osteogenic sarcomas (Farrands et al, 1983) and colorectal carcinomas (Farrands et al, 1982;Armitage et al, 1983;Pimm et al, 1984). In studies on colorectal cancer, autoradiography of tumours resected from patients given 1311-labelled 791T/36 antibody has indicated that the antibody predominantly localizes in stromal elements and secretions within pseudoacini rather than showing a clear cut binding to malignant cells (Armitage et al, 1983).…”