Localisation of phosphatidylethanolamine-binding protein in the brain and other tissues of the rat

Frayne, Jan; Ingram, C.D.; Love, Seth; Hall, Len

doi:10.1007/s004410050064

Cited by 58 publications

(34 citation statements)

References 23 publications

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“…Highest levels were seen in testes and brain, followed by liver and kidney. These observations are in good agreement with a prior analysis in rat tissues [12]. No detectable RKIP-1 protein was observed in any tissue from RKIP-1 −/− animals (not shown), with the exception of a low signal in testis (Fig.…”

Section: Expression Of the Rkip Gene Family In The Mousesupporting

confidence: 92%

“…This phenotype may be evolutionarily reminiscent of the RKIP-1 function previously seen in Drosophila [12] and plants [33]. In Drosophila, RKIP orthologues reside in antennae and olfactory hairs and play a role in odorant binding.…”

Section: Discussionmentioning

confidence: 56%

“…The functions of RKIP-1 in a variety of organisms are diverse and include inhibition of carboxypeptidase Y in yeast [5], an immunoprotective function in nematodes [12], odorant binding and detection in Drosophila [12], and control of shoot growth and flowering in plants [33]. In mammals, signaling proteins are often linked to disease states [22], and recent work indicates that RKIP-1 is a metastasis suppressor [7,13,19,31].…”

Section: Introductionmentioning

confidence: 99%

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Raf kinase inhibitory protein knockout mice: Expression in the brain and olfaction deficit

Theroux

Pereira

Casten

et al. 2007

Brain Research Bulletin

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Raf Kinase Inhibitory Protein (RKIP-1) is involved in the regulation of the MAP kinase, NF-κB, and GPCR signaling pathways. It is expressed in numerous tissues and cell types and orthologues have been documented throughout the animal and plant kingdoms. RKIP-1 has also been reported as an inhibitor of serine proteases, and a precursor of a neurostimulatory peptide. RKIP-1 has been implicated as a suppressor of metastases in several human cancers. We generated a knockout strain of mice to further assess RKIP-1's function in mammals. RKIP-1 is expressed in many tissues with the highest protein levels detectable in testes and brain. In the brain, expression was ubiquitous in limbic formations, and homozygous mice developed olfaction deficits in the first year of life. We postulate that RKIP-1 may be a modulator of behavioral responses.

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Section: Expression Of the Rkip Gene Family In The Mousesupporting

confidence: 92%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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Raf kinase inhibitory protein knockout mice: Expression in the brain and olfaction deficit

Theroux

Pereira

Casten

et al. 2007

Brain Research Bulletin

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“…Subsequent studies revealed that PEBP/RKIP is a widely expressed and highly conserved protein that does not share any significant homology with any known protein family, with homologs in, E.Coli, A.thaliana, S.cerevisae, C.elegans, and D. melanogaster, which display a high degree of interspecies similarity [9].…”

Section: Rkipmentioning

confidence: 99%

“…In Drosophila, which expresses at least 7 RKIP isoforms, RKIP/PEBPs were hypothesized to play a role in odorant binding, as the proteins are localized in antennae and secreted into the lumen of olfactory hairs [9].…”

Section: Rkipmentioning

confidence: 99%

Modulation of the MAP kinase signaling cascade by Raf kinase inhibitory protein

Trakul

Rosner

2005

Cell Res

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Proteins like Raf kinase inhibitory protein (RKIP) that serve as modulators of signaling pathways, either by promoting or inhibiting the formation of productive signaling complexes through protein-protein interactions, have been demonstrated to play an increasingly important role in a number of cell types and organisms. These proteins have been implicated in development as well as the progression of cancer. RKIP is a particularly interesting regulator, as it is a highly conserved, ubiquitously expressed protein that has been shown to play a role in growth and differentiation in a number of organisms and can regulate multiple signaling pathways. RKIP is also the first MAP kinase signaling modulator to be identified as playing a role in cancer metastasis, and identification of the mechanism by which it regulates Raf-1 activation provides new targets for therapeutic intervention.

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Protein extraction and 2‐DE of water‐ and lipid‐soluble proteins from bovine pericardium, a low‐cellularity tissue

et al. 2008

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Bovine pericardium (BP) is an important biomaterial used in the production of glutaraldehyde-fixed heart valves and tissue-engineering applications. The ability to perform proteomic analysis on BP is useful for a range of studies, including investigation of immune rejection after implantation. However, proteomic analysis of fibrous tissues such as BP is challenging due to their relative low-cellularity and abundance of extracellular matrix. A variety of methods for tissue treatment, protein extraction, and ;fractionation were investigated with the aim of producing high-quality 2-DE gels for both water- and lipid-soluble BP proteins. Extraction of water-soluble proteins with 3-(benzyldimethylammonio)-propanesulfonate followed by n-dodecyl β-d-maltoside extraction and ethanol precipitation for lipid-soluble proteins provided the best combination of yield, spot number, and resolution on 2-DE gels (Protocol E2). ESI-quadrupole/ion trap or MALDI-TOF/TOF MS protein identifications were performed to confirm bovine origin and appropriate subcellular prefractionation of resolved proteins. Twenty-five unique, predominantly cytoplasmic bovine proteins were identified from the water-soluble fraction. Thirty-two unique, predominantly membrane bovine proteins were identified from the lipid-soluble fraction. These results demonstrated that the final protocol produced high-quality proteomic data from this important tissue for both cytoplasmic and membrane proteins.

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Localisation of phosphatidylethanolamine-binding protein in the brain and other tissues of the rat

Cited by 58 publications

References 23 publications

Raf kinase inhibitory protein knockout mice: Expression in the brain and olfaction deficit

Raf kinase inhibitory protein knockout mice: Expression in the brain and olfaction deficit

Modulation of the MAP kinase signaling cascade by Raf kinase inhibitory protein

Protein extraction and 2‐DE of water‐ and lipid‐soluble proteins from bovine pericardium, a low‐cellularity tissue

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