Localization and abundance of early markers of fat cell differentiation in the skeletal muscle of cattle during growth — Are DLK1-positive cells the origin of marbling flecks?

Albrecht, Elke; Kuzinski, Judith; Komolka, Katrin; Gotoh, Takafumi; Maak, Steffen

doi:10.1016/j.meatsci.2014.10.012

Cited by 20 publications

(15 citation statements)

References 41 publications

(53 reference statements)

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“…In addition to standard hematoxylin/eosin (H/E) staining, immunohistochemistry was applied for UCP1 and C/EBPβ detection on 12 µm thick frozen tissue sections (2–3 randomly selected mice per group) using antibodies against either UCP1 (1:100, sc-6529, Santa Cruz) for 1 h at room temperature or C/EBPβ (1:100, sc-150, Santa Cruz) for 2 h at room temperature in a humidity chamber similar to previously described procedure (Albrecht et al 2015a). Specific binding was detected with fluorescence-labeled secondary antibodies (MFP 488 rabbit anti- goat IgG for UCP1 and MFP 590 goat anti-rabbit IgG for C/EBPβ, MoBiTec, Goettingen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

et al. 2016

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For the assessment of genetic or conditional factors of fat cell browning, novel and polygenic animal models are required. Therefore, the long-term selected polygenic mouse line DUhTP originally established in Dummerstorf for high treadmill performance is used. DUhTP mice are characterized by increased fat accumulation in the sedentary condition and elevated fat mobilization during mild voluntary physical activity. In the present study, the phenotype of fat cell browning of subcutaneous fat and a potential effect on oral glucose tolerance, an indicator of metabolic health, were addressed in DUhTP mice. Analysis of peripheral fat pads revealed increased brite (brown-in-white) subcutaneous adipose tissues and in subcutaneous fat from DUhTP mice higher levels of irisin and different markers of fat cell browning like T-box transcription factor (Tbx1), PPARα, and uncoupling protein (UCP1) (P < 0.05) when compared to unselected controls. UCP1 was further increased in subcutaneous fat from DUhTP mice in response to mild exercise (fourfold, P < 0.05). In addition, surface temperature of DUhTP mice was increased when compared to controls indicating a physiological effect of increased UCP1 expression. The present study suggests that DUhTP mice exhibit different markers of mitochondrial biogenesis and fat browning without external stimuli. At an age of 43 days, sedentary DUhTP mice have improved metabolic health as judged from lower levels of blood glucose after an oral glucose tolerance test. Consequently, the non-inbred mouse model DUhTP represents a novel model for the identification of fat cell browning mechanisms in white adipose tissues.

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Section: Methodsmentioning

confidence: 99%

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

et al. 2016

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“…Many tissues of human and animal origin have been employed as sources of ASC, e.g. subcutaneous [2], intraperitoneal [3], muscle [4], visceral [5] and bone marrow tissues [6]. Thus, a wide array of adult adipose tissues can serve as a pool for adipocyte progenitors.…”

Section: Introductionmentioning

confidence: 99%

Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction

et al. 2020

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Adipocyte differentiation of bovine adipose-derived stem cells (ASC) was induced by foetal bovine serum (FBS), biotin, pantothenic acid, insulin, rosiglitazone, dexamethasone and 3-isobutyl-1-methylxanthine, followed by incubation in different media to test the influence of ascorbic acid (AsA), bovine serum lipids (BSL), FBS, glucose and acetic acid on transdifferentiation into functional adipocytes. Moreover, different culture plate coatings (collagen-A, gelatin-A or poly-L-lysine) were tested. The differentiated ASC were subjected to Nile red staining, DAPI staining, immunocytochemistry and quantitative reverse transcription PCR (for NT5E, THY1, ENG, PDGFRα, FABP4, PPARγ, LPL, FAS, GLUT4). Nile red quantification showed a significant increase in the development of lipid droplets in treatments with AsA and BSL without FBS. The presence of BSL induced a prominent increase in FABP4 mRNA abundance and in FABP4 immunofluorescence signals in coincubation with AsA. The abundance of NT5E, ENG and THY1 mRNA decreased or tended to decrease in the absence of FBS, and ENG was additionally suppressed by AsA. DAPI fluorescence was higher in cells cultured in poly-L-lysine or gelatin-A coated wells. In additional experiments, the multi-lineage differentiation potential to osteoblasts was verified in medium containing ß-glycerophosphate, dexamethasone and 1,25-dihydroxyvitamin D 3 using alizarin red staining. In conclusion, bovine ASC are capable of multi-lineage differentiation. Poly-L-lysine or gelatin-A coating, the absence of FBS, and the presence of BSL and AsA favour optimal transdifferentiation into adipocytes. AsA supports transdifferentiation via a unique role in FABP4 induction, but this is not linearly related to the primarily BSL-driven lipid accumulation.

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“…Numerous studies have demonstrated that Dlk1 plays an important role in regulating skeletal muscle development and regeneration [4], differentiation of adipocytes [5,6], and blood cells formation [7]. Dlk1 is also involved in certain cancers [8], wound repair [9], obesity [10], and several signaling pathways [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Delta-like 1 homolog in Capra hircus: molecular characteristics, expression pattern and phylogeny

Zhao

Zhan

et al. 2016

Mol Biol Rep

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To research the molecular characteristics, expression pattern and phylogeny of the Delta-like 1 homolog gene (Dlk1) in goats. Dlk1 transcripts were identified in the Jianyang Da'er goats by reverse-transcription polymerase chain reaction (RT-PCR). Phylogenetic trees were constructed by Bayesian inference and neighbor-joining methods. Quantitative real-time PCR (qPCR), western blotting and in situ hybridization were performed to analyze the expression pattern of Dlk1. Five alternatively transcripts were identified in different tissues and designated as Dlk1-AS1, 2, 3, 4 and 5. Compared with the normal transcript Dlk1-AS1, Dlk1-AS4 and Dlk1-AS5 retained the identical open reading frame (ORF) and encoded proteins with truncated epidermal-growth-factor like repeats of 121 and 83 amino acids, respectively. Using the Bayesian inference method, the consensus phylogenetic tree indicated that caprine Dlk1 had a closer relationship with bovine Dlk1 than with Dlk1 from pigs, humans and mice. qPCR revealed high expression levels of Dlk1 in the kidney (P < 0.01). However, mRNA and protein levels presented an inconsistent correlation, possibly because of post-transcriptional regulation. RNA in situ hybridization indicated that Dlk1 mRNA was localized in the interlobular bile duct and alongside the hepatocyte nuclei, in the epithelial cells of proximal and distal convoluted tubules and in the connective region between the mesothelium and myocardium in the heart. The Dlk1 gene in goats produces alternatively spliced transcripts, with specific expression and cellular localization patterns. These findings would lay the foundation for further study.

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Localization and abundance of early markers of fat cell differentiation in the skeletal muscle of cattle during growth — Are DLK1-positive cells the origin of marbling flecks?

Cited by 20 publications

References 41 publications

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

Browning of subcutaneous fat and higher surface temperature in response to phenotype selection for advanced endurance exercise performance in male DUhTP mice

Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction

Delta-like 1 homolog in Capra hircus: molecular characteristics, expression pattern and phylogeny

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