Localization and Alteration of Immunoreactive Endothelin-1 in Canine Basilar Arteries Following Subarachnoid Hemorrhage.

Kobayashi, Masahiko; Nishikibe, Masaru; Maruyama, Hiromi; Yano, Mitsuo; Ikemoto, Fumihiko; Ide, Katsuhisa; Itoh, Seiga; Sasaki, Tatsuya

doi:10.1267/ahc.28.129

Cited by 13 publications

(2 citation statements)

References 32 publications

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“…On the other hand, in cerebral vasospasm models of other species, the ppET-1 showed some increase in protein level. 12,32 However, the increased level of ppET-1 was not extraordinary, at only 1.7 to approximately 2.2 times above the normal level. This suggests that ppET-1 could act solely as a trigger in the early stage of vasospasm or that its biological constriction effect is not proportional to the amount of protein itself.…”

Section: Fig 5 Southern Blot Analysismentioning

confidence: 88%

Inhibition of vascular contraction by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA in a rat experimental vasospasm model

Onoda¹,

Ono²,

Ogihara³

et al. 1996

Journal of Neurosurgery

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To clarify the role of endothelin-1 (ET-1) in the etiology of hemolysate-induced contraction of vessels, the authors introduced antisense oligoDNA for preproendothelin-1 (ppET-1) messenger RNA in a rat model of vasospasm. Phosphorothioate antisense oligoDNAs for ppET-1 were injected into the cisterna magna. Fluorescein isothiocyanate-labeled phosphorothioate antisense oligoDNAs were proven by fluorescence chasing to be incorporated into the vascular wall. Striking inhibitory effects of experimental vasospasm were observed in the basilar artery (BA) in which the oligoDNAs were injected. The vascular contraction was significantly inhibited by oligoDNAs after 20 minutes of hemolysate exposure, which suggested that ET synthesis started approximately 20 minutes after hemolysate stimulation. Expression of ppET-1 in the BA in which the spasm was inhibited was markedly suppressed at the transcription level. The results indicate that ET-1 may play an important role in hemolysate-induced vasoconstriction in rats. In addition, the antisense approach in the cerebrospinal fluid might be a useful tool for preventing cerebral vasospasm.

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Section: Fig 5 Southern Blot Analysismentioning

confidence: 88%

Inhibition of vascular contraction by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA in a rat experimental vasospasm model

Onoda¹,

Ono²,

Ogihara³

et al. 1996

Journal of Neurosurgery

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show abstract

“…The sections were cut into 3-p~m widths and stained immunohistochemically using the biotin-streptoavidin (BSA) horseradish peroxidase method for the detection of ET-1. Details of the intrnunohistochemical methods appear in our previous work [17]. In brief, rabbit anti-ET-1 polyclonal antibody and rabbit anti-rat macrophage antibody were used as the primary antibody.…”

Section: Immunohistochemicai Analysis For Et-1 Staining In the Lungsmentioning

confidence: 99%

Distribution of endothelin-1 in the lung of rats with pulmonary hypertension of different etiology

Sakai¹,

Miyauchi²,

Kusumoto³

et al. 1998

Int J Angiol

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Distribution of endothelin-1 in the lung of rats with pulmonary hypertension of different etiology

et al. 2011

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Localization and Alteration of Immunoreactive Endothelin-1 in Canine Basilar Arteries Following Subarachnoid Hemorrhage.

Cited by 13 publications

References 32 publications

Inhibition of vascular contraction by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA in a rat experimental vasospasm model

Inhibition of vascular contraction by intracisternal administration of preproendothelin-1 mRNA antisense oligoDNA in a rat experimental vasospasm model

Distribution of endothelin-1 in the lung of rats with pulmonary hypertension of different etiology

Distribution of endothelin-1 in the lung of rats with pulmonary hypertension of different etiology

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