Localization and distribution of nitric oxide synthase and other neuronal markers in the podia of <i>Holothuria arguinensis</i>

Marquet, Nathalie; Canário, Adelino V. M.; Power, Deborah M.

doi:10.1111/ivb.12298

Cited by 3 publications

(10 citation statements)

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Section: Discussionmentioning

confidence: 90%

“…We have previously found that sea cucumbers maintained a reduced number of GPCR ORs when compared to other echinoderms (Marquet, Canário, et al, 2020;Marquet, Cardoso, et al, 2020). In H. arguinesis and in A. japonicus, four OR types were found (totalling 57 and 79 ORs, respectively) from an assembly of sensory and reproductive tissues, compared to around 300 identified in A. planci and S. purpuratus (Marquet, Canário, et al, 2020;Marquet, Cardoso, et al, 2020). The iGluR repertoire in H. arguinesis, A. japonicus and other echinoderm genomes is much smaller than that of ORs, and gene number is relatively well conserved, albeit expanded in H. arguinesis and A. japonicus.…”

Section: A Putative Chemosensory Role For Gluhmentioning

confidence: 98%

“…Echinoderms do not have a brain, and their simple central nervous system (CNS) consists of a circular nerve ring and radial nerve cords (Díaz‐Balzac & García‐Arrarás, 2018; Hoekstra et al, 2012). In sea cucumbers the nerve ring is localized at the base of tentacles, a highly sensory and enervated organ, from which radial nerve cords exit and enervate the body wall (Díaz‐Balzac et al, 2010; Marquet et al, 2020; Marquet, Cardoso, et al, 2020; San Miguel‐Ruiz et al, 2009). As a step towards identifying chemosensory neurons in sea cucumbers, we have mapped neurons that use nitric oxide and other signalling mechanisms in H .…”

Section: Introductionmentioning

confidence: 99%

“…As a step towards identifying chemosensory neurons in sea cucumbers, we have mapped neurons that use nitric oxide and other signalling mechanisms in H. arguinensis (Marquet, Canário, et al, 2020). We have also characterized the olfactory receptor-like (OR-like) GPCR repertoire and identified 79 in the Apostichopus japonicus genome and 57 in tissue transcriptomes of H. arguinensis (Marquet, Canário, et al, 2020;Marquet, Cardoso, et al, 2020). The OR-like repertoire of H. arguinesis is expressed mostly in tentacles, the oral cavity and papillae/tegument and is smaller than in other echinoderms (Strongylocentrotus purpuratus and Acanthaster planci; Hall et al, 2017;Marquet, Canário, et al, 2020;Marquet, Cardoso, et al, 2020;Raible et al, 2006;Roberts et al, 2017;Sea Urchin Genome Sequencing et al, 2006), suggesting that mediation and transmission of environmental chemical cues in echinoderms may be regulated by different molecular mechanisms.…”

mentioning

confidence: 99%

“…In sea cucumbers the nerve ring is localized at the base of tentacles, a highly sensory and enervated organ, from which radial nerve cords exit and enervate the body wall (Díaz-Balzac et al, 2010;Marquet, Cardoso, et al, 2020;San Miguel-Ruiz et al, 2009). As a step towards identifying chemosensory neurons in sea cucumbers, we have mapped neurons that use nitric oxide and other signalling mechanisms in H. arguinensis (Marquet, Canário, et al, 2020). We have also characterized the olfactory receptor-like (OR-like) GPCR repertoire and identified 79 in the Apostichopus japonicus genome and 57 in tissue transcriptomes of H. arguinensis (Marquet, Canário, et al, 2020;Marquet, Cardoso, et al, 2020).…”

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confidence: 99%

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A new subfamily of ionotropic glutamate receptors unique to the echinoderms with putative sensory role

et al. 2021

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Chemosensation is a critical signalling process in animals and especially important in sea cucumbers, a group of ecologically and economically important marine echinoderms (Class Holothuroidea), which lack audio and visual organs and rely on chemical sensing for survival, feeding and reproduction. The ionotropic receptors are a recently identified family of chemosensory receptors in insects and other protostomes, related to ionotropic glutamate receptor family (iGluR) a large family of membrane receptors in metazoan. Here we characterize the echinoderm iGluR subunits and consider their possible role in chemical communication in sea cucumbers. Sequence similarity searches revealed that sea cucumbers have in general a higher number of iGluR subunits when compared to other echinoderms. Phylogenetic analysis and sequence comparisons revealed GluH as a specific iGluR subfamily present in all echinoderms. Homologues of the vertebrate GluA (aka αamino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, AMPA), GluK (aka kainate) and GluD (aka delta) were also identified. The GluN (aka N-methyl-d-aspartate, NMDA) as well as the invertebrate deuterostome subfamily GluF (aka phi) are absent in echinoderms. The echinoderm GluH subfamily shares conserved structural protein organization with vertebrate iGluRs and the ligand binding domain (LBD) is the most conserved region and genome analysis indicates evolution via lineage and speciesspecific tandem gene duplications. GluH genes (named Grih) are the most expressed iGluRs subunit genes in tissues in the sea cucumber Holothuria arguinesis, with Griha1, Griha2 and Griha5 exclusively expressed in tentacles, making them candidates to have a chemosensory role in this species. The multiple GluH subunits may provide alternative receptor assembly combinations, thus expanding the functional possibilities and widening the range of compounds detected during aggregation and spawning in echinoderms.

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Section: Discussionmentioning

confidence: 90%

Section: A Putative Chemosensory Role For Gluhmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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A new subfamily of ionotropic glutamate receptors unique to the echinoderms with putative sensory role

et al. 2021

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Chemical ecology of sea cucumber reproduction

Marquet,

Canário

2024

The World of Sea Cucumbers

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Sea cucumbers: an emerging system in evo-devo

Perillo,

Sepe,

Paganos

et al. 2024

EvoDevo

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A challenge for evolutionary developmental (evo-devo) biology is to expand the breadth of research organisms used to investigate how animal diversity has evolved through changes in embryonic development. New experimental systems should couple a relevant phylogenetic position with available molecular tools and genomic resources. As a phylum of the sister group to chordates, echinoderms extensively contributed to our knowledge of embryonic patterning, organ development and cell-type evolution. Echinoderms display a variety of larval forms with diverse shapes, making them a suitable group to compare the evolution of embryonic developmental strategies. However, because of the laboratory accessibility and the already available techniques, most studies focus on sea urchins and sea stars mainly. As a comparative approach, the field would benefit from including information on other members of this group, like the sea cucumbers (holothuroids), for which little is known on the molecular basis of their development. Here, we review the spawning and culture methods, the available morphological and molecular information, and the current state of genomic and transcriptomic resources on sea cucumbers. With the goal of making this system accessible to the broader community, we discuss how sea cucumber embryos and larvae can be a powerful system to address the open questions in evo-devo, including understanding the origins of bilaterian structures.

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Localization and distribution of nitric oxide synthase and other neuronal markers in the podia of Holothuria arguinensis

Abstract: Echinoderms are slow-moving and mostly broadcast-spawning marine invertebrates which have very limited or a total lack of vision and audition. Although they react to light and touch (e.g., Ullrich

Cited by 3 publications

References 67 publications

A new subfamily of ionotropic glutamate receptors unique to the echinoderms with putative sensory role

A new subfamily of ionotropic glutamate receptors unique to the echinoderms with putative sensory role

Chemical ecology of sea cucumber reproduction

Sea cucumbers: an emerging system in evo-devo

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