Localization and dynamics of Mel1a melatonin receptor in the ovary of carp Catla catla in relation to serum melatonin levels

Chattoraj, Asamanja; Seth, Mohua; Maitra, Saumen Kumar

doi:10.1016/j.cbpa.2008.11.010

Cited by 44 publications

(30 citation statements)

References 54 publications

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“…The protein content of samples, used for electrophoresis and western blot analysis, was analyzed on 12.5% Laemmli SDS-PAGE and was visualized by 0.1% Coomassie brilliant blue (overnight) followed by destaining or processed for immunoblotting on the PVDF membrane by a wet electroblotting method (Chattoraj et al , 2009b. The PVDF membranes were incubated separately with primary antibody (1:1000 dilution) raised against a peptide corresponding to an amino acid sequence at the carboxy terminus of cyclin B1 of rat origin or rabbit anti-PSTAIRE (for p34Cdk1) or primary polyclonal antibodies against MT1 followed by incubation with the respective secondary antibody (dilution 1:500).…”

Section: Electrophoresis and Immunoblottingmentioning

confidence: 99%

“…However, an in vitro study has demonstrated for the first time ) that melatonin may act locally on the carp ovary, as prior incubation of denuded oocytes with melatonin augments the action of maturation-inducing hormone (MIH) or 17a,20b-dihydroxy-4-pregnen-3-one (17a,20b-DP) on oocyte maturation by the formation of maturation-promoting factor (MPF) -a complex of two proteins, cyclin B and cyclin-dependent kinase Cdk1 (Yamashita et al 1992). Subsequent localization and dynamics of a 37 kDa melatonin receptor protein in the oocytes of the same fish (Chattoraj et al 2009b) also argue in favor of extra-hypothalamic actions of melatonin. The study on zebrafish reports that melatonin plays a critical role in promoting seasonal reproduction by activating the cascade starting from the kiss peptide, which, in turn, stimulates hypothalamic neurons to produce gonadotropin-releasing hormone (GnRH), and by acting directly on gonads that regulate the transcription of two important genes whose proteins are involved in oocyte competence and maturation (Carnevali et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Moniruzzaman¹,

Hasan²,

Maitra³

2016

Reproduction

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The major objective of the present study was to demonstrate the actions of exogenous melatonin on ovaprim (synthetic GnRH and domperidone)-induced final oocyte maturation focusing on the oxidative status of pre-ovulatory follicles in the carp Catla catla. Accordingly, gravid carp during the early spawning phase of the reproductive cycle were injected with melatonin and/or ovaprim at different time intervals or luzindole (a pharmacological blocker of melatonin receptors) before their administration. We studied their effects on the latency period, the rate of germinal vesicle breakdown (GVBD; a visual marker of final oocyte maturation) in oocytes, and the levels of maturation-promoting factor (MPF), as well as oxidative stress, different antioxidants, melatonin and MT1 melatonin receptor protein in the extracts of pre-ovulatory follicles. Notably, melatonin treatment 2 h before the injection of ovaprim resulted in the shortest latency period as well as the highest rate of GVBD and MPF formation. Exogenous melatonin, irrespective of the injection schedule, caused a significant reduction in intra-follicular oxidative stress and an increase in the levels of both enzymatic and nonenzymatic antioxidants, melatonin and its receptor protein. Concentrations of ovarian melatonin in each fish exhibited a significant negative correlation with the level of oxidative stress, but a positive correlation with the rate of GVBD and the activity/level of different antioxidants. However, no significant effects of melatonin and/or ovaprim were detected in luzindole-pretreated carp. Collectively, the present study provides the first evidence that melatonin pretreatment in carp ameliorates ovaprim actions on the process of final oocyte maturation by the formation of MPF and alleviates oxidative stress in pre-ovulatory follicles by stimulating different antioxidants.

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Section: Electrophoresis and Immunoblottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Moniruzzaman¹,

Hasan²,

Maitra³

2016

Reproduction

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“…Each diluted primary antibody was mixed with the respective immunizing antigen (10 μg/ml) and pre-absorbed for 12 h at 4°C before incubation of the immunoblot (Huang et al, 2005;Yaoi et al, 2001). Individual band intensity of the Western blots was quantified by densitometry analysis using Image J software (Chattoraj et al, 2009). …”

Section: Western Blot Analysismentioning

confidence: 99%

IMPORTANCE OF LIGHT IN TEMPORAL ORGANIZATION OF PHOTORECEPTOR PROTEINS AND MELATONIN-PRODUCING SYSTEM IN THE PINEAL OF CARPCATLA CATLA

Seth

Maitra

2010

Chronobiology International

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The importance of light in the temporal organization of photoreceptor proteins and melatonin-producing system has been investigated for the first time in the pineal of a tropical fish. In this study, an identical experimental paradigm was followed during the four distinct phases of an annual cycle in adult carps (Catla catla) maintained either under natural photoperiod (NP) or continuous illumination (LL) or darkness (DD) for 30 days. At the end of each experiment, the pineal from fish in each experimental group was collected either at 06:00, 12:00, 18:00, or 24:00 in a daily cycle and assessed by Western blot analysis for pineal rod-like opsin, alpha-transducin, and AANAT. The same animals were also used for measurement of serum melatonin levels, and the serum as well as intra-pineal Ca(++) levels at each timepoint. The study revealed a daily rhythmicity with a peak at 12:00 h and nadir at 24:00 h in the band intensity of pineal rod-like opsin and alpha-transducin in NP fish, while the band intensities of these photo-pigment proteins remained high under LL and low under DD, irrespective of clock hour during the 24 h cycle. The band intensity of pineal AANAT, levels of serum melatonin, and both serum Ca(++) and intra-pineal Ca(++) were maximum at 24:00 h and minimum at 12:00h in NP fish, and they were significantly lower under LL and higher under DD at each point of study. The results showed loss of daily rhythm in each studied variable in both LL and DD carps, suggesting that their circadian organization is dependent on the external light-dark conditions, rather than an endogenous circadian oscillator in the pineal.

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“…Moreover, the reproductive status and the internal hormonal milieu are also known to modulate the melatonin receptor expression (Chattoraj et al 2009;Yadav et al 2011). The trade-off relationship existing between gonadal/ adrenal steroids and melatonin along with their receptors is widely accepted in seasonally breeding rodents Ahmad and Haldar 2010;Martin et al 2008).…”

Section: Photoperiodic Regulation Of Melatonin Receptor Expressionmentioning

confidence: 99%

Nycthemeral variation in melatonin receptor expression in the lymphoid organs of a tropical seasonal breeder Funambulus pennanti

Gupta

Haldar

2014

J Comp Physiol A

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Seasonal variations in immune functions point toward the involvement of melatonin in its regulation. These chronobiotic effects are exerted by receptors present on the immunocompetent cells. The present study investigated daily/nycthemeral variation in expression of melatonin receptor subtypes MT1/MT2 in the lymphoid organs (spleen/thymus) of a tropical squirrel, Funambulus pennanti. The receptor expression was noted every 4 h interval over 24 h under natural light-dark cycle, during two seasons and was correlated with peripheral level of melatonin. The MT1/MT2 receptor expression displayed higher levels at the time of dusk (light-dark transition; 1800 hours), while plasma melatonin was still low compared to the preceding time point. The receptors were downregulated during the nighttime with a minimum expression at 0200 hours. Thymus, during the long day length, showed a tissue-specific pattern of receptor expression with a minimum expression at 0600 hours. Results suggest that photoperiod by modulation of melatonin level inversely regulates the receptor expression. The observations imply that there exists a temporal window of sensitivity in the target organs to the melatonin signal that is regulated by modulation of melatonin receptor expression which might be involved in mediating the photoperiodic effects of melatonin in the control of seasonal immune physiology.

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Localization and dynamics of Mel1a melatonin receptor in the ovary of carp Catla catla in relation to serum melatonin levels

Cited by 44 publications

References 54 publications

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

IMPORTANCE OF LIGHT IN TEMPORAL ORGANIZATION OF PHOTORECEPTOR PROTEINS AND MELATONIN-PRODUCING SYSTEM IN THE PINEAL OF CARPCATLA CATLA

Nycthemeral variation in melatonin receptor expression in the lymphoid organs of a tropical seasonal breeder Funambulus pennanti

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