Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni

Reis, Mitermayer Galvão dos; Kuhns, Janet; Blanton, Ronald E.; Davis, Alan H.

doi:10.1016/0166-6851(89)90062-5

Cited by 40 publications

(25 citation statements)

References 21 publications

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“…Fs800, has already been shown to be up-regulated in the vitelline cells during maturation of female worms and has been linked to the production and maintenance of eggs [42]. The gynecophoral canal protein (SMU47862), also previously shown to be differentially expressed between male and females, has been implicated in male/female communication or interaction [44].…”

Section: Resultsmentioning

confidence: 99%

Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni

et al. 2007

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Background: The parasitic trematode Schistosoma mansoni is one of the major causative agents of Schistosomiasis, a disease that affects approximately 200 million people, mostly in developing countries. Since much of the pathology is associated with eggs laid by the female worm, understanding the mechanisms involved in oogenesis and sexual maturation is an important step towards the discovery of new targets for effective drug therapy. It is known that the adult female worm only develops fully in the presence of a male worm and that the rates of oviposition and maturation of eggs are significantly increased by mating. In order to study gene transcripts associated with sexual maturation and oviposition, we compared the gene expression profiles of sexually mature and immature parasites using DNA microarrays.

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Section: Resultsmentioning

confidence: 99%

Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni

et al. 2007

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“…In schistosomes, the vitelline cells are haploid, suggesting a common origin for the vitelline gland and the ovary (Basch and Samuelson 1990). Vitelline cells probably play a key role in eggshell formation and a reduced importance in embryo nourishment (Erasmus 1975;Erasmus et al 1982;Bobek et al 1986;Johnson et al 1987;Koster et al 1988;Reis et al 1989;Chen et al 1992;Schussler et al 1995;Fitzpatrick et al 2004Fitzpatrick et al , 2007. The eggshell has cribriform-like pores, which allow the uptake of exogenous nutrients during egg migration through host tissues (Lewert et al 1970;Stjernholm and Warren 1974;Neill et al 1988;Swiderski 1994).…”

Section: Discussionmentioning

confidence: 97%

The embryonic development of Schistosoma mansoni eggs: proposal for a new staging system

et al. 2009

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Schistosomiasis is a water-borne parasitic illness caused by neoophoran trematodes of the genus Schistosoma. Using classical histological techniques and whole-mount preparations, the present work describes the embryonic development of Schistosoma mansoni eggs in the murine host and compares it with eggs maintained under in vitro conditions. Two pre-embryonic stages occur inside the female worm: the prezygotic stage is characterized by the release of mature oocytes from the female ovary until its fertilization. The zygotic stage encompasses the migration of the zygote through the ootype, where the eggshell is formed, to the uterus. Fully formed eggs are laid still undeveloped, without having suffered any cleavage. In the outside environment, eight embryonic stages can be defined: stage 1 refers to early cleavages and the beginning of yolk fusion. Stage 2 represents late cleavage, with the formation of a stereoblastula and the onset of outer envelope differentiation. Stage 3 is defined by the elongation of the embryonic primordium and the onset of inner envelope formation. At stage 4, the first organ primordia arise. During stages 5 to 7, tissue and organ differentiation occurs (neural mass, epidermis, terebratorium, musculature, and miracidial glands). Stage 7 is characterized by the nuclear condensation of neurons of the central neural mass. Stage 8 refers to the fully formed larva, presenting muscular contraction, cilia, and flame-cell beating. This staging system was compared to a previous classification and could underlie further studies on egg histoproteomics (morphological localizome). The differentiation of embryonic structures and their probable roles in granulomatogenesis are discussed herein.

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“…Other previous studies have compared differential gene expression between females and males, and similar results have also been reported. For example, egg antigen [5,40,41], ferritin-1 [5,40,42,43], ribosomal proteins [5,40,44], ATPase [44,45], cathepsin [45], extracellular superoxide dismutase [5,43,44], cytochrome C oxidase [5], tyrosinase [43], mucin-like protein [46], fs800 [47], and adenylosuccinate lyase [5], among others, are often detected in females. These up-regulated genes in paired females are considered to play roles in female sexual maturation and egg production.…”

Section: Discussionmentioning

confidence: 99%

Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing

Sun

Wang

et al. 2014

Parasit Vectors

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BackgroundSchistosoma japonicum is one of the major causative agents of schistosomiasis. The pairing of males and females leads to female sexual maturation and maintains this mature state. However, the mechanisms by which pairing facilitates sexual maturation are yet to be investigated.MethodsParasites isolated from single- and double-sex cercariae-infected mice were analyzed by Solexa to uncover pair-regulated miRNA profiles. To reveal the biological functions of differentially expressed miRNAs among the samples, we predicted the target genes of these differentially expressed miRNAs and compared the gene expression between 23-d-old female schistosomula from double-sex infections (23DSI) and 23-d-old female schistosomula from single-sex infections (23SSI) by analyzing digital gene expression profiling (DGE). KEGG pathway analysis was used to investigate the relevant biological processes of these target genes to understand the significance of differentially expressed miRNAs after pairing.ResultsThe differentially expressed miRNA profiles of female 18- and 23-d post-single- and double-sex infections were analysed by Solexa. Similar miRNA profiles were observed in 18SSI and 18DSI, with the presence of identically expressed high-abundance miRNA, such as miRNA-1, miRNA-71b-5p and let-7. By contrast, in 23DSI and 23SSI, most of these high-abundance miRNAs were down-regulated. Furthermore, among all samples, bantam was distinctly up-regulated in 23 DSI, and miR-1, miR-71, miR-7-5p, and miR-7 were distinctly up-regulated in 23SSI. The transcriptomes of 23DSI and 23SSI revealed that the predicted target genes of miRNA-1, miRNA-71, miRNA-7, and miR-7-5p were associated with the ribonucleoprotein complex assembly and microtubule-based process. Conversely, the predicted target genes of bantam were related to the embryo development, development of primary sexual characteristics and regulation of transcription. KEGG pathway analysis revealed that in unpaired females, the highly-expressed miRNA-1, miRNA-71, miRNA-7, and miR-7-5p only inhibited the limited pathways, such as proteasome and ribosome assembly. Meanwhile, in paired mature females, highly-expressed bantam inhibited more biological pathways, such as the citrate cycle, glycolysis, fatty acid biosynthesis and RNA degradation.ConclusionsThe differentially expressed miRNAs between 23SSI and 23DSI and their different functions indicated that more genes or metabolic pathways in paired mature females were inhibited than those in unpaired ones. The results suggested that after pairing, specific miRNAs regulated gene expression to lead to female sexual maturation.

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Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni

Cited by 40 publications

References 21 publications

Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni

Microarray analysis of gene expression induced by sexual contact in Schistosoma mansoni

The embryonic development of Schistosoma mansoni eggs: proposal for a new staging system

Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing

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