Localization and Phenotype of Resident Macrophages in the Dental Pulp during Rat Mandibular First Molar Development

Miyauchi, Yasunori; Mayahara, Mitsuori; Sasa, Ryuji; Inoue, Mitsuko; Nakamura, Masanori

doi:10.7881/dentalmedres.30.15

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…M2 macrophages may play a role in homeostasis maintenance of the engineered tissue, as resident macrophages do in normal dental pulp (Luke et al 2013). The localization and phenotype of the resident macrophages were reported to change during rat tooth development (Miyauchi et al 2010), which indicates a regulatory function of resident macrophages in uninflamed dental pulp. A previous study has also shown that the immunoreactivity of macrophages appeared to change during the pulpal regeneration process in injured dental pulp (Nakakura-Ohshima et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Macrophage populations show an M1‐to‐M2 transition in an experimental model of coronal pulp tissue engineering with mesenchymal stem cells

Kaneko

Zaw

et al. 2018

Int Endodontic J

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Aim To assess M1/M2 macrophage phenotypes in a coronal pulp regeneration model in rats, under the hypothesis that there are dynamic M1/M2 phenotype changes during the different stages of the pulp regeneration. Methodology The maxillary first molars of Wistar rats were pulpotomized, and biodegradable hydrogel‐made scaffolds carrying rat bone marrow mesenchymal stem cells were implanted in the pulp chamber. After 3, 7 and 14 days, samples were processed for (i) histological analysis and double immunoperoxidase staining for CD68 (a general macrophage marker) and one of either CCR7 (an M1 marker), CD163 (an M2 marker) or CD206 (an M2 marker); (ii) real‐time PCR for AIF1 (an M1 marker), CD163, CD206, IL‐10 and TNF‐α mRNA expression; and (iii) Western blotting for the detection of CD68, CCR7 and CD206 proteins. Results Histological analysis of the implanted region revealed sparse cellular distribution at 3 days, pulp‐like tissue with a thin dentine bridge‐like structure at 7 days, and dentine bridge‐like mineralized tissue formation and resorption of most scaffolds at 14 days. CCR7+ macrophages had the highest density at 3 days, and then significantly decreased until 14 days (P < 0.05). In contrast, M2 marker (CD163 or CD206) expressing macrophages had the lowest density at 3 days and significantly increased until 14 days (P < 0.05). AIF1 and TNF‐α mRNA levels, and CD68 and CCR7 protein levels were highest at 3 days. CD163 and CD206 mRNA levels, and CD206 protein levels increased with time and showed the highest at 14 days. IL‐10 mRNA was highest at 3 days, decreased at 7 days and increased at 14 days. Conclusions Macrophages in the regenerating pulp tissue underwent a distinct transition from M1‐dominant to M2‐dominant, suggesting that the M1‐to‐M2 transition of macrophages plays an important role in creating a favourable microenvironment necessary for pulp tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Macrophage populations show an M1‐to‐M2 transition in an experimental model of coronal pulp tissue engineering with mesenchymal stem cells

Kaneko

Zaw

et al. 2018

Int Endodontic J

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“…However, macrophages in the rat crown dental pulp and the root pulp express nonidentical immunological profiles. Researchers have suggested that alternation from CD68 + /MHC class II − to CD68 + /MHC class II + occurs during the maturation of dental pulp macrophages (Miyauchi et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

et al. 2020

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Studying the dermal skeleton in fish is valuable for phylogenetic specification. The current study describes the detailed structure of the plecostomus dermal skeleton, including its morphogenesis and distribution in the skin. The denticles have a crown and a basal part and are embedded in bony depressions, to which they are attached by denticle ligaments. During denticle morphogenesis, denticle papillae formed from denticle precursor cells align in two cellular layers: an outer ameloblast precursor layer and an inner odontoblast precursor layer. The ameloblast precursors and odontoblast precursors differentiate and secrete enamel and dentine, respectively. We used different histochemical techniques, including Crossmon's trichrome staining, Weigert–Van Gieson staining, periodic acid–Schiff (PAS) staining, combined Alcian blue (AB; pH 2.5)/PAS staining, Weigert–Van Gieson staining, Mallory trichrome staining, and AB staining to distinguish the dentine and denticle ligaments. We used acridine orange to detect lysosome activity during denticle eruption. Transmission electron microscopy was used to detect the denticle ultrastructure, and scanning electron microscopy was used to detect the topographic distributions of different types of dermal tissues in different anatomical regions.

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“…The dental pulp is susceptible to damage resulting from dental caries, dental trauma, and surgical procedures, amongst others. However, the dental pulp is well-equipped with several tissue-resident macrophage populations that can mount a defense response, mitigate in ammation, and accelerate the healing process [19][20][21][22] . Our previous study demonstrated the existence of a LYVE-1 + macrophage population in rat dental pulp tissue under steadystate conditions 23 .…”

Section: Introductionmentioning

confidence: 99%

Kinetics of LYVE-1-Positive M2-Like Macrophages in Developing and Repairing Dental Pulp in Vivo and Their Pro-Angiogenic Activity in Vitro

Thoai

Tazawa

Kawashima

et al. 2022

Preprint

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Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological function of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling.

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Localization and Phenotype of Resident Macrophages in the Dental Pulp during Rat Mandibular First Molar Development

Cited by 5 publications

References 22 publications

Macrophage populations show an M1‐to‐M2 transition in an experimental model of coronal pulp tissue engineering with mesenchymal stem cells

Macrophage populations show an M1‐to‐M2 transition in an experimental model of coronal pulp tissue engineering with mesenchymal stem cells

Morphological and Histochemical Characterization of the Dermal Plates of Pleco (Hypostomus plecostomus)

Kinetics of LYVE-1-Positive M2-Like Macrophages in Developing and Repairing Dental Pulp in Vivo and Their Pro-Angiogenic Activity in Vitro

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