Localization and uptake of 14C-IAA in relation to xylem regeneration in Coleus internodes

Abstract

14C-IAA was applied to decapitated Coleus 5th internodes. Sections were radioautographed at various intervals after the application. The location of the label within cells and tissues is discussed. No accumulation of label occurred in the region where xylem cells regenerated. Label was particularly associated with xylem and phloem cytoplasm, but also occurred in other cells.

Hormone transport and action in the green shoot: long‐term studies of a clonal stock of Coleus blumei (Labiatae)

“…Already differentiated tracheary cells (i.e., those radially farther from the cambium), as well as young tracheary cells longitudinally farther from the site of IAA application, are not labeled. Similar re-sults were later reported from other Coleus stocks (Wangermann, 1970;Gee, 1972).…”

Primary phloem regeneration without concomitant xylem regeneration: controlling factors in Coleus blumei

“…The first label over the phloem was seen after 24 hours, and after 48 hours the cytoplasm and slime material were the predominantly labelled regions of the phloem. Further studies with Coleus using14 C-IAA showed heavy labelling in the phloem near the sieve plates and slime plugs, and the label could be removed by leaching(Wangermann, 1968(Wangermann, , 1970Gee, 1972). These re searches in Coleus indicate the possible involvement of phloem in the translocation of lAA.…”