Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Sharkey, Robert M.; Hagihara, Patrick F.; Goldenberg, D. M.

doi:10.1038/bjc.1977.25

Cited by 19 publications

(3 citation statements)

References 24 publications

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“…CA 19-9 (U/mg w.wt.) and mean CA 19-9 concentrations (2 _+ SEM) in normal large-bowel mucosa, colorectal adenomas, and colorectal carcinomas in colorectal adenomas and carcinomas [4,7,[18][19][20]. Consequently, in tissue differentiation into "benign" and "malignant" on the basis of the tumor-associated antigens CEA and CA 19-9 is not possible qualitatively, but at best quantitatively.…”

Section: Discussionmentioning

confidence: 99%

“…The risk of malignant degeneration increases with size of the adenoma, the extent of its villous component and of its cellular atypia present. Immunohistochemically, both CEA and CA 19-9 can be detected in colorectal adenomas [4][5][6][7]. The present study was undertaken in an attempt to determine whether there is any relationship between the tissue concentrations of CEA and CA 19-9 in colorectal adenomas, on the one hand, and their size and histological and cytologic structure, on the other.…”

Section: Introductionmentioning

confidence: 99%

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Tissue concentrations of CEA and CA 19-9 in the carcinogenesis of colorectal carcinoma exemplified by the adenoma-carcinoma sequence

Fischbach

Mössner

1988

Res. Exp. Med.

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To study the behavior of the tumor-associated antigens CEA and CA 19-9 in colorectal carcinogenesis, exemplified by the adenoma-carcinoma sequence, their tissue concentrations were measured in adenomas of different size and histology and compared with those found in normal colonic mucosa and carcinoma. Both in the case of monoclonal and polyclonal CEA assay, significantly higher concentrations were found in the adenoma tissue as compared with normal mucosa. Carcinomas revealed, on average, an even higher tissue CEA level, but the concentrations measured showed considerable scatter. In the adenoma group, the villous lesions and those with severe cellular atypia were characterized by markedly higher CEA concentrations, reflecting their special position in the adenoma-carcinoma sequence. In the case of CA 19-9, too, an increase in tissue concentrations from normal mucosa through adenoma to carcinoma was observed. In contrast, among the adenomas of different histologies and dysplasia no differences were observed. These findings fit with the concept of the adenoma-carcinoma sequence which, in our opinion, represents a suitable model for studying the significance of tumor-associated antigens in the carcinogenesis of colorectal cancer and its precursors.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tissue concentrations of CEA and CA 19-9 in the carcinogenesis of colorectal carcinoma exemplified by the adenoma-carcinoma sequence

Fischbach

Mössner

1988

Res. Exp. Med.

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“…It is in this group of patients, and particularly in those with concentrations above 20 ng/ml, that production of CEA by the tumour itself, in much the same way as occurs with colonic carcinomas, seems likely. Proof of this mechanism could be provided by demonstrating CEA in the tumour tissue using immunoperoxidase or immunofluorescence, or by estimating the CEA content of the tumour by radioimmunoassay, as has been done with other tumours (Sharkey et al, 1977). The carcino-foetal protein most often produced by HCC is AFP.…”

Section: Resultsmentioning

confidence: 99%

Carcinoembryonic antigen in hepatocellular cancer

Macnab¹,

Urbanowicz²,

Kew³

1978

Br J Cancer

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Summary.-Serum carcinoembryonic antigen (CEA) concentrations were found to be raised in 28 of 72 black patients (390/) with hepatocellular cancer (HCC). The degree of elevation was slight or moderate, except in 3 patients in whom values >20 ng/ml were recorded. No significant correlation could be demonstrated in individual patients between the serum CEA concentration and various tests of liver function. The mean CEA value in the patients with cirrhosis in the non-tumorous liver was slightly higher than that in those without cirrhosis, but the difference did not reach statistical significance. There was no correlation between serum CEA and a-foetoprotein (AFP) levels.

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Immunocytochemical detection of carcinoembryonic antigen in conventional histopathology specimens

Goldenberg

Sharkey

Primus

1978

Cancer

146

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The 3-step, unlabeled antibody, immunoperoxidase method for staining CEA in conventional histopathology sections was used to evaluate the presence of CEA in 950 different specimens, the vast majority of which were available as paraffin-embedded tissues fixed in formalin. The sensitivity of the method clearly discriminated between normal or nonmalignant, diseased tissues and neoplasms having increased quantities of CEA. Thus, tumors of epithelial origin, particularly adenocarcinomas or squamous cell carcinomas of the gastrointestinal tract, bronchus, ovary, and cervix, were frequently prone to CEA staining. Among ovarian tumors, there appeared to be a distinct proclivity of CEA staining in mucinous as compared to serous cystadenocarcinomas. The only normal tissues showing CEA were colonic mucosae adjacent or near to benign or malignant tumors, suggesting CEA absorption from the neoplasm and not primarily de novo CEA biosynthesis. With the exception of inflammatory conditions of the colon, nonmalignant, diseased tissues did not have CEA demonstrable by this method. It is proposed that immunocytochemical staining of CEA in conventional tumor pathology sections can be used to discriminate those cases in which blood CEA titers need to be determined on a serial basis in order to monitor recurrence or progression, since a positive immunoperoxidase reaction for CEA in the primary tumor predicts well in many cases which patients will have a blood CEA value reflecting disease activity. Furthermore, the excellent correlation found for CEA staining between primary and metastatic tumors of the same cases suggests that the immunocytochemical method could have diagnostic potential for detecting micrometastatic spread of CEApositive cancers to regional lymph nodes, thus aiding in the screening of regional lymph nodes of cancer patients.

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Localization by immunoperoxidase and estimation by radioimmunoassay of carcinoembryonic antigen in colonic polyps

Cited by 19 publications

References 24 publications

Tissue concentrations of CEA and CA 19-9 in the carcinogenesis of colorectal carcinoma exemplified by the adenoma-carcinoma sequence

Tissue concentrations of CEA and CA 19-9 in the carcinogenesis of colorectal carcinoma exemplified by the adenoma-carcinoma sequence

Carcinoembryonic antigen in hepatocellular cancer

Immunocytochemical detection of carcinoembryonic antigen in conventional histopathology specimens

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