Localization of acidic fibroblast growth factor (aFGF) mRNA in mouse and bovine retina by in situ hybridization

Jacquemin, E.; Halley, C.; Alterio, Jeanine; Laurent, M.; Courtois, Yves; Jeanny, Jean‐Claude

doi:10.1016/0304-3940(90)90380-r

Cited by 40 publications

(16 citation statements)

References 20 publications

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“…In rat brain, FGF2 is present in most neurons within the cerebral cortex (1), hippocampus (2), and cerebellum (3). High levels of FGF1 expression have been observed in motor neurons, primary sensory neurons, and retinal ganglion neurons (4,5). In chick brain, the expression of FGF1 is developmentally regulated (6).…”

Section: Fgf1mentioning

confidence: 99%

“…B, analysis by RT-PCR of rat FGF1 transcripts. RNA from PC12 cells (1), MDA-MB-231 (2), differentiated B12, B18 (3,4), and undifferentiated B7 (5)-transfected cells were assayed by RT-PCR using rFGF1S1S and rFGF1AS primers. Amplified products were analyzed as in A except that hybridization was performed with an internal rat specific FGF1 primer.…”

Section: Expression Of Fgf1 Transcripts During Differentiation Of Pc1mentioning

confidence: 99%

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The Neurotrophic Activity of Fibroblast Growth Factor 1 (FGF1) Depends on Endogenous FGF1 Expression and Is Independent of the Mitogen-activated Protein Kinase Cascade Pathway

Renaud

Desset

Oliver

et al. 1996

Journal of Biological Chemistry

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The expression of fibroblast growth factor (FGF) 1, a potent neurotrophic factor, increases during differentiation and remains high in adult neuronal tissues. To examine the importance of this expression on the neuronal phenotype, we have used PC12 cells, a model to study FGF-induced neuronal differentiation. After demonstrating that FGF1 and FGF2 are synthesized by PC12 cells, we investigated if FGF1 expression could be a key element in differentiation. Using the cell signaling pathway to determine the effects of FGF1 alone, FGF1 plus heparin, or a mutated FGF1, we showed an activation to the same extent of mitogen-activated protein (MAP) kinase kinase and MAP kinase (extracellular regulated kinase 1). However, only FGF1 plus heparin could promote PC12 cell differentiation. Thus, the MAP kinase pathway is insufficient to promote differentiation. Analysis of the PC12 cells after the addition of FGF1 plus heparin or FGF2 demonstrated a significant increase in the level of FGF1 expression with the same time course as the appearance of the neuritic extensions. Transfection experiments were performed to enhance constitutivly or after dexamethasone induction the level of FGF1 expression. The degree of differentiation achieved by the cells correlated directly with the amount of FGF1 expressed. The MAP kinase pathway did not appear to be involved. Interestingly, a 5-fold increase in FGF1 in constitutive transfected cells extended dramatically their survival in serum-free medium, suggesting that the rise of FGF1 synthesis during neuronal differentiation is probably linked to their ability to survive in the adult. All of these data demonstrate that, in contrast to the MAP kinase cascade, FGF1 expression is sufficient to induce in PC12 cells both differentiation and survival. It also shows that auto-and trans-activation of FGF1 expression is involved in the differentiation process stimulated by exogenous FGFs through a new pathway which remains to be characterized. FGF11 and 2 are widely distributed in the peripheral and central nervous systems in the adult. In rat brain, FGF2 is present in most neurons within the cerebral cortex (1), hippocampus (2), and cerebellum (3). High levels of FGF1 expression have been observed in motor neurons, primary sensory neurons, and retinal ganglion neurons (4, 5). In chick brain, the expression of FGF1 is developmentally regulated (6). In bovine and rat embryonic retina, all neuronal layers express FGF1 with an appearance corresponding to their sequential differentiation (7, 8). In rat, the level of FGF1 expression remains uniformly low throughout the embryonic period until postnatal day 7. Thereafter, it increases rapidly, reaching a maximum in the adult retina. In the intermediate central nervous system, subclasses of FGF receptors appear to be down-regulated during development (9), and during retinal embryonic development, the expression of FGFR1 and FGFR2 follows the retinal layering (10). These patterns of FGF expression suggest that these growth factors are involved in the integrit...

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Section: Fgf1mentioning

confidence: 99%

Section: Expression Of Fgf1 Transcripts During Differentiation Of Pc1mentioning

confidence: 99%

The Neurotrophic Activity of Fibroblast Growth Factor 1 (FGF1) Depends on Endogenous FGF1 Expression and Is Independent of the Mitogen-activated Protein Kinase Cascade Pathway

Renaud

Desset

Oliver

et al. 1996

Journal of Biological Chemistry

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“…Addition of haFGF did not increase the survival of cultured septal neurons from both E16 and P15. Messenger RNA of aFGF has been detected in retina (8), but its dis tribution in various regions of the brain has not been sufficiently elucidated. From our pres ent data, the responsibilities to aFGF might vary according to the age of the animals and it might be seen in restricted regions of the brain, although the effects of bFGF might not be specific to neurons from various regions of brain.…”

Section: Discussionmentioning

confidence: 99%

“…The fibroblast growth factor (FGF) family consists of at least seven closely related polypeptide mitogens which exert their activities by bind ing and activation of specific cell surface re ceptors (1 3), and they have mitogenic activ ities for various types of cells (4,5). FGFs are localized in the central nervous system (6)(7)(8), and they promote the survival and neurite out growth of cultured neurons from various brain regions (9 14). Basic FGF (bFGF) prevents the death of lesioned cholinergic neurons in vivo (15,16).…”

mentioning

confidence: 99%

“…Basic FGF (bFGF) prevents the death of lesioned cholinergic neurons in vivo (15,16). Acidic FGF (aFGF) has been isolated from whole brain, hypothalamus and retina (4,6,17), and its mRNA has been de tected in retina (8). Acidic FGF also has neurotrophic effects on various regions of neurons in the brain, but its effective concen trations are considerably higher than those of bFGF (12).…”

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confidence: 99%

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Human Acidic Fibroblast Growth Factor Has Trophic Effects on Cultured Neurons from Multiple Regions of Brain and Retina.

et al. 1991

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Neurotrophic activities of human recombinant acidic fibroblast growth factor (haFGF) were evaluated on primary cultured neurons from various brain regions and compared with those of CS23, modified human basic fibroblast growth factor. Survival of cultured neurons from embryonic day 16 (E16) rat cortex and sub stantia nigra was significantly increased by the addition of more than 10 ng/ml haFGF and that from the hippocampus was increased by 100 and 1000 ng/ml. However, en hancement of viability by haFGF was observed only in 1000 ng/ml-treated neurons from the striatum, thalamus, colliculus and cerebellum; and it was not observed in septal neurons. Survival of cultured neurons from postnatal day 15 rat on glial feeder layer was significantly increased by the addition of 1000 ng/ml haFGF, except for neurons from the septum. CS23 (10 ng/ml) increased the survival of cultured neurons from all regions mentioned above, and its effects were stronger than those of 1000 ng/ml haFGF. Addition of more than 10 ng/ml haFGF significantly increased the sur vival of cultured neurons from neonatal day 2 rat retina. Addition of 1000 ng/ml haFGF increased the choline acetyl transferase activity of E16 septal neurons slightly but significantly, but didn't increase the dopamine uptake activity of embryonic day E15 ventral midbrain. These results show that haFGF is effective on limited regions and ages of brain and retina, while CS23 is effective on all regions tested.

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Acidic Fibroblast Growth Factor (FGF-1) and FGF Receptor 1 Signaling in Human Y79 Retinoblastoma

Siffroi-Fernandez

Cinaroglu²,

Fuhrmann-Panfalone³

et al. 2005

Arch Ophthalmol

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Objectives: Fibroblast growth factors (FGFs) represent potent effectors and play essential roles in both normal development and many pathological processes. Little is known about their possible implication in retinoblastoma growth. We sought to examine FGF high-and lowaffinity receptor (FGFR) expression, activation of FGFR1 by acidic FGF (FGF-1), and proliferative effects on Y79 cells. Methods: Expression of FGFR1 to FGFR4 was screened in Y79 cells by means of immunochemical and reverse transcriptase polymerase chain reaction techniques. Tyrosine phosphorylation of FGFR1 induced by FGF was examined by immunoprecipitation after stimulation with FGF-1 in the presence or absence of heparin. Retinoblastoma proliferation was monitored by radiolabeled thymidine incorporation or a vital dye-based assay, after addition of FGF-1 with or without inclusion of a specific FGFR1 neutralizing antibody or FGFR1 antisense oligonucleotides. Low-affinity heparan sulfate proteoglycan coreceptors were blocked through sodium chlorate or heparinase treatment of Y79 cells. Results: Y79 retinoblastoma expressed all 4 FGFRs, at both the protein and messenger RNA levels. The FGFR1 was differentially phosphorylated in a time-and heparindependent manner by FGF-1. Proliferation of Y79 cells induced by FGF-1 was entirely mediated by FGFR1, since inclusion of specific neutralizing antibodies or antisense oligonucleotides completely prevented tumor cell multiplication. Finally, FGF-1-induced proliferation was dependent on the presence and sulfation of heparan sulfate proteoglycan. Conclusions: Y79 retinoblastoma expresses all 4 FGFRs, but FGFR1 activation entirely accounts for FGF-1-driven cell proliferation. Clinical Relevance: These studies demonstrate a role for the FGF-1/FGFR1 pathway in retinoblastoma proliferation, and may contribute to developing therapeutic strategies to limit retinoblastoma growth.

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Localization of acidic fibroblast growth factor (aFGF) mRNA in mouse and bovine retina by in situ hybridization

Cited by 40 publications

References 20 publications

The Neurotrophic Activity of Fibroblast Growth Factor 1 (FGF1) Depends on Endogenous FGF1 Expression and Is Independent of the Mitogen-activated Protein Kinase Cascade Pathway

The Neurotrophic Activity of Fibroblast Growth Factor 1 (FGF1) Depends on Endogenous FGF1 Expression and Is Independent of the Mitogen-activated Protein Kinase Cascade Pathway

Human Acidic Fibroblast Growth Factor Has Trophic Effects on Cultured Neurons from Multiple Regions of Brain and Retina.

Acidic Fibroblast Growth Factor (FGF-1) and FGF Receptor 1 Signaling in Human Y79 Retinoblastoma

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