Localization of angiotensin II receptor subtypes in the rabbit adrenal and kidney

Herblin, William F.; Diamond, Sheila; Timmermans, Pieter B.M.W.M.

doi:10.1016/0196-9781(91)90105-x

Cited by 33 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In general, autoradiography after in vitro radioligand binding reveals similar patterns of distribution of AT1 receptors in most adult mammalian kidneys, including rat, rabbit, guinea pig, monkey, and humans (53)(54)(55)(56)(57)(58)(59)(60). AT1 receptors are most prominent in three different anatomical regions of the kidney, namely glomeruli, the interglomerular area of the cortex corresponding to the proximal convoluted tubules, and throughout the inner stripe of the outer medulla, where AT1 receptor binding is most intense in longitudinal bands traversing this zone in rat (Fig.…”

Section: An Overview Of Intrarenal Localization Of Ang II Receptor Sumentioning

confidence: 99%

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

et al. 1997

View full text Add to dashboard Cite

The renal medulla plays an important role in maintaining body fluid and electrolyte balance and longterm blood pressure homeostasis through its unique structural and functional properties. Among several humoral, paracrine factors or autocoids, angiotensin II (Ang II) has been implicated in the regulation of renal medullary function, including the medullarylpapillary microcirculation, urine concentration, and blood pressure, but the mechanisms by which Ang II exerts influences in the renal medulla are largely unknown. The purpose of this review is to summarize the cellular localization, regulation, and functional properties of Ang II AT1 receptors in the kidney, with special emphasis on type I renomedullary interstitial cells (RMICs) in the renal medulla and cultured RMICs. High densities of AT1 receptors have been localized in type I RMICs in the inner stripe of the outer medulla by high resolution light and electron microscopic autoradiography following in vitro or in vivo labelling, or in cultured RMICs. Furthermore, reverse transcription polymerase chain reaction and Southern blot analysis now confirm that AT1 receptors in cultured RMICs are exclusively of the AT1A subtype. In cultured RMICs, Ang II markedly increases intracellular inositol 1,4,5-triphosphate (IP3) concentration, and stimulates cell proliferation and extracellular matrix synthesis, and these cellular responses are exclusively mediated by AT1 receptors. Considering the co-occurrence of high levels of renin, renin substrate angiotensinogen, and Ang II in the interstitial fluid compartment, and AT1 receptors in type I RMICs of the renal medulla, the AT1 receptor-bearing RMICs may be more responsive to the locally formed interstitial Ang II than to the circulating peptide. Since RMICs also contain the receptors for other vasoactive peptides, such as endothelin (ETA and ETB), natriuretic peptides (NPRA and NPRB), and bradykinin (B2), and synthesize prostaglandins and medullipins, they may serve as an important site for functional interactions between Ang II and other vasoactive peptides in modulating renal medullary function. More studies using different experimental approaches are therefore required to explore and elucidate the functional role of renal interstitial Ang II and AT1 receptors in RMICs in the physiological control of renal medullary function and in the pathophysiology of hypertension and progressive renal diseases. (Hypertens Res 1997; 20: 233-250)

show abstract

Section: An Overview Of Intrarenal Localization Of Ang II Receptor Sumentioning

confidence: 99%

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

et al. 1997

View full text Add to dashboard Cite

show abstract

“…In cultured VSMC, there is now good evidence that the trophic effects of All are mediated through the stimulation of an AT1 receptor subtype Bunkenburg et al, 1992). On the other hand, the pathophysiological role of the AT2 receptor subtype is not yet well understood (Criscione et al, 1990;Dudley et al, 1990;Herblin et al, 1991;Timmermans et al, 1991;Bottari et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the angiotensin II AT₁ receptor subtype involved in DNA synthesis in cultured vascular smooth muscle cells

Briand¹,

Riva²,

Galzin³

1994

British J Pharmacology

View full text Add to dashboard Cite

“…However, autoradiography revealed that in the rabbit the fibrous sheath around the kidney contained AT2-R-binding sites [20]and that in the rhesus monkey AT2-R-binding sites were present in juxtaglomerular apparatus and in vasculature in the renal cortex [6]. With respect to localization of Ang II receptor subtypes in the adult human kidney, all studies were performed at the protein level by autoradiography using [ 125 I]Ang II as a ligand [8, 10, 11], and distribution patterns at mRNA levels have not yet been analyzed.…”

Section: Introductionmentioning

confidence: 99%

Tissue-Specific Expression of Human Angiotensin II AT1 and AT2 Receptors and Cellular Localization of Subtype mRNAs in Adult Human Renal Cortex Using in situ Hybridization

Matsubara

Sugaya

Murasawa

et al. 1998

Nephron

View full text Add to dashboard Cite

All studies analyzing the localization of angiotensin II (Ang II) receptors in the human kidney have been performed at the protein level using ¹²⁵I-Ang II as a probe. In this study, cellular localizations of Ang II type l (AT1-R) and type 2 (AT2-R) receptor mRNAs in the adult human renal cortex were examined for the first time using in situ hybridization, and their expression patterns determined by RNase protection assay were compared with those in other human tissues. In the human renal cortex obtained from tumor-free portions in renal cell carcinoma, AT1-R mRNA levels were about 8- to 10-fold higher than AT2-R mRNA levels. Human liver and aorta predominantly expressed AT1-R mRNA, while human right atrium contained both AT1-R and AT2-R mRNAs. Ligand-binding assays revealed that the total Ang II receptor number in the human renal cortex was 16.0 ± 3.3 fmol/mg protein, similar to that in liver (17.7 ± 5.8) but significantly higher than in right atrium (11.6 ± 3.2) and aorta (5.6 ± 2.7). Relative distribution ratios of AT1-R and AT2-R numbers in the renal cortex and right atrium were 82/17 and 56/42%, respectively. In situ hybridization study indicated that strongest AT1-R mRNA signals were located in interlobular arteries and tubulointerstitial fibrous regions surrounding interlobular arteries and glomeruli, followed in decreasing order by glomeruli and cortical tubules. Expression of AT2-R mRNA was highly localized in interlobular arteries. Cells present in tubulointerstitial regions were positive for vimentin and collagen type 1, indicating that the majority of the cells present in the regions are fibroblasts. Presence of strong AT1-R mRNA signals in the tubulointerstitial fibrous regions surrounding arteries and glomeruli and the expression of AT2-R mRNA in the interlobular artery were the first evidence, suggesting a pharmacological framework for the differential effects of Ang II receptor subtype mediated renal function in the adult human kidney.

show abstract

Localization of angiotensin II receptor subtypes in the rabbit adrenal and kidney

Cited by 33 publications

References 6 publications

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

Characterization of the angiotensin II AT₁ receptor subtype involved in DNA synthesis in cultured vascular smooth muscle cells

Tissue-Specific Expression of Human Angiotensin II AT1 and AT2 Receptors and Cellular Localization of Subtype mRNAs in Adult Human Renal Cortex Using in situ Hybridization

Contact Info

Product

Resources

About

Localization of angiotensin II receptor subtypes in the rabbit adrenal and kidney

Cited by 33 publications

References 6 publications

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

Localization and Functional Properties of Angiotensin II AT1 Receptors in the Kidney. Focus on Renomedullary Interstitial Cells.

Characterization of the angiotensin II AT1 receptor subtype involved in DNA synthesis in cultured vascular smooth muscle cells

Tissue-Specific Expression of Human Angiotensin II AT1 and AT2 Receptors and Cellular Localization of Subtype mRNAs in Adult Human Renal Cortex Using in situ Hybridization

Contact Info

Product

Resources

About

Characterization of the angiotensin II AT₁ receptor subtype involved in DNA synthesis in cultured vascular smooth muscle cells