Localization of ATPase in bovine retinal outer segments

Hemminki, Kari

doi:10.1016/0014-4835(75)90110-4

Cited by 14 publications

(10 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 . Whatever their origin, their presence could account for the reports (6,13,21) of Na+,K+-ATPase activity in isolated outer segments.…”

Section: Results Inmentioning

confidence: 95%

[3H]ouabain autoradiography of frog retina.

Stirling¹,

Lee²

1980

The Journal of Cell Biology

View full text Add to dashboard Cite

The kinetics and distribution of ouabain binding in retinas of Rana pipiens were examined quantitatively by scintillation counting and freeze-dry autoradiography . Binding within the proximal receptor layers was also nonuniform . Several peaks were observed, with those at the inner segment and synaptic layers being especially noticeable . Assuming an absence of glial cell binding in the proximal receptor layers, we calculated there to be 13 X 10 6 ouabain or Na + ,K+ pump sites per rod receptor . Limited measurements suggest a BmaX of -8 X 10-6 M for the inner plexiform layer .

show abstract

“…11 . Whatever their origin, their presence could account for the reports (6,13,21) of Na+,K+-ATPase activity in isolated outer segments.…”

Section: Results Inmentioning

confidence: 95%

[3H]ouabain autoradiography of frog retina.

Stirling¹,

Lee²

1980

The Journal of Cell Biology

View full text Add to dashboard Cite

show abstract

“…In contrast to these physiological experiments, biochemical studies of Na-K-ATPase activity in the photoreceptor cells have proposed two wholly different results concerning this enzyme distribution at the outer segments. One was that the outer segments possessed a high activity (9,19,28), and the other was that this reported activity in the outer segments came from contamination by the inner segments when using cell fractionation methods (6,11). To detect the localization of the Na-K-ATPase, radioautography, immunocytochemical technique and histochemical method are available.…”

Section: Discussionmentioning

confidence: 99%

“…The site of Na-K-ATPase is shown to be present mainly in the plasma membrane of the inner segments and more proximal portion of the photoreceptor cells by these studies. On the other hand, some biochemical studies of Na-K-ATPase activity using cell fractionation methods claimed that the outer segments possessed a high activity (9,17,28). Hence, the Na-K-ATPase pump localization has so far been a controversial problem.…”

mentioning

confidence: 99%

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

Ueno

Mayahara

Tsukahara

et al. 1980

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…Mc MAS T r RJ RSIT branes to Na+a &(13, )M-phenomena that occur or are proposed to occur in retinal rods (17)(18)(19).…”

Section: Health Sciences Librarymentioning

confidence: 99%

Dopamine Auto- and Postsynaptic Receptors: Electrophysiological Evidence for Differential Sensitivity to Dopamine Agonists

Skirboll

Grace

Bunney

1979

Science

491

121

View full text Add to dashboard Cite

The responses of dopamine cells in the substantia nigra to iontophoretically administered dopamine and intravenous apomorphine were compared to the responses of spontaneously active neurons in the caudate nucleus. Dopaminergic cells were six to ten times more sensitive to dopamine and intravenous apomorphine than 86 percent of the caudate cells tested. This differential sensitivity of dopamine auto- and postsynaptic receptors may explain the apparently paradoxical behavioral effects induced by small compared to large doses of some dopamine agonists and may provide a means of developing new types of drugs to antagonize dopaminergic influence in the central nervous system.

show abstract

Localization of ATPase in bovine retinal outer segments

Cited by 14 publications

References 18 publications

[3H]ouabain autoradiography of frog retina.

[3H]ouabain autoradiography of frog retina.

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

Dopamine Auto- and Postsynaptic Receptors: Electrophysiological Evidence for Differential Sensitivity to Dopamine Agonists

Contact Info

Product

Resources

About