Localization of calcitonin binding sites in rat central nervous system: Evidence of its neuroactivity

Olgiati, Vincenzo R.; Guidobono, F.; Netti, C.; Pecile, A.

doi:10.1016/0006-8993(83)90334-7

Cited by 116 publications

(25 citation statements)

References 21 publications

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“…Previous evidence has shown that SCT, when administered either centrally or peripherally, inhibits PRL release in rats (Olgiati et al 1981(Olgiati et al , 1982(Olgiati et al , 1983. In vitro experiments from this and other laboratories have extended these earlier findings by demonstrating that SCT acts directly at the level of lactotropes to inhibit PRL release, PRL gene transcription and lactotrope cell proliferation (Shah et al 1988, 1999, Judd et al 1990, Sortino et al 1991, Zhang et al 1995.…”

Section: Gct1-asct-absupporting

confidence: 60%

Calcitonin is expressed in gonadotropes of the anterior pituitary gland: its possible role in paracrine regulation of lactotrope function

Ren¹,

Chien²,

Sun³

et al. 2001

Journal of Endocrinology

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Previous studies from this laboratory have shown that salmon (S) calcitonin (CT)-like immunoreactive peptide (CTI) is synthesized and secreted by the anterior pituitary (AP) gland. These studies also co-localized CTI to gonadotropes, and demonstrated that SCT is a potent inhibitor of lactotrope function. However, the molecular structure of putative gonadotrope-derived CTI that inhibits lactotrope function has not been defined.The present studies cloned CT cDNA (pit-CT cDNA) from a mouse gonadotrope L T2 cell line using RT-PCR and rapid amplification of cDNA ends (RACE) techniques. Alignment of nucleotide sequences of pit-CT and mouse CT revealed greater than 99% homology between the sequences. The pit-CT cDNA was ligated into a mammalian expression vector, and the construct was transfected into L T2 cells. Two stable transfectant cell lines (CT.U6/A and B) were obtained by selection in G418. Subsequent S1-nuclease protection assay and immunocytochemistry results have shown that: (1) pit-CT peptide expressed by CT.U6 cell lines immunoreacted with GCT1-anti-SCT serum; (2) secretions of CT.U6 cells inhibited prolactin (PRL) release, PRL mRNA abundance and DNA synthesis of PRL-secreting GGH3 cells; and (3) CT.U6-induced inhibition was abolished by GCT1-anti-SCT serum. The studies also generated a riboprobe from the cloned pit-CT cDNA, and localized CT mRNA expression in gonadotropes of rat AP gland by in situ hybridization histochemistry.These results demonstrate that pit-CT mRNA is closely homologous to mouse CT mRNA; it is expressed by gonadotropes of the rat AP gland, and the peptide may significantly affect lactotrope function by inhibiting PRL release and cell proliferation.

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Section: Gct1-asct-absupporting

confidence: 60%

Calcitonin is expressed in gonadotropes of the anterior pituitary gland: its possible role in paracrine regulation of lactotrope function

Ren¹,

Chien²,

Sun³

et al. 2001

Journal of Endocrinology

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“…Amylin binding has been reported in each of these brain areas, accompanied by calcitonin receptor protein and/or mRNA (Olgiati et al, 1983;Sexton et al, 1994a,b;Christopoulos et al, 1995;Hilton et al, 1995;Skofitsch et al, 1995;Nakamoto et al, 2000;Ueda et al, 2001;Stachniak and Krukoff, 2003;Barth et al, 2004;Becskei et al, 2004;Eftekhari and Edvinsson, 2011). The presence of RAMPs and thus functional amylin receptor subtypes is subject to the same caveats described above.…”

Section: E Expression and Physiologic Relevance Of Amylin Receptor Smentioning

confidence: 96%

Amylin: Pharmacology, Physiology, and Clinical Potential

Hay¹,

Chen²,

Lutz³

et al. 2015

Pharmacol Rev

302

297

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“…Furthermore, it was suggested that CGRP might be an endogenous ligand for calcitonin receptors in the central nervous system and concluded that calcitonin and CGRP may interact on the same receptor site. A detailed comparison of previously published autoradiographic studies of calcitonin-binding sites [7,20] could not be made. However, similarities of localization of binding sites in large areas could be seen.…”

Section: Discussionmentioning

confidence: 99%

Autoradiographic distribution of 125I calcitonin gene-related peptide binding sites in the rat central nervous system

Skofitsch¹,

Jacobowitz²

1985

Peptides

144

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SKOFITSCH, G. AND D. M. JACOBOWITZ. Autoradiographic distribution of v'-:'l calcitonin gene-related peptide binding sites in the rat central nervous system. PEPTIDES 6(5) [975][976][977][978][979][980][981][982][983][984][985][986] 1985.--Using autoradiographic method and "-':'I-Tyr ° rat CGRP as a ligand, receptor binding sites were demonstrated in the rat central nervous system. Saturation studies and Scatchard analysis of CGRP-binding to slide mounted tissue sections containing primarily cerebellum showed a single class of receptors with a dissociation constant of 0.96 nM and a Bm,~x of 76.4 fmol/mg protein, v-'~I-Tyr ° rat CGRP binding sites were demonstrated throughout the rat central nervous system. Dense binding was observed in the telencephaIon (medial prefrontal, insular and outer layers of the temporal cortex, nucleus accumbens, fundus striatum, central and inferior lateral amygdaloid nuclei, most caudal caudate putamen, organum vasculosum laminae terminalis, subfornical organ), the diencephalon (anterior hypothalamic, suprachiasmatic, arcuate, paraventricular, dorsomedial, periventricular, reuniens, rhomboid, lateral thalamic pretectalis and habenula nuclei, zona incerta), in the mesencephalon (superficial layers of the superior colliculus, central nucleus of the geniculate body, inferior colliculus, nucleus of the fifth nerve, locus coeruleus, nucleus of the mesencephalic tract, the dorsal tegmental nucleus, superior olive), in the molecular layer of the cerebellum, in the medulla oblongata (inferior olive, nucleus tractus solitarii, nucleus commissuralis, nuclei of the tenth and twelfth nerves, the prepositus hypoglossal and the gracilis nuclei, dorsomedial part of the spinal trigeminal tract), in the dorsal gray matter of the spinal cord (laminae I-VI) and the confines of the central canal. Moderate receptor densities were found in the septal area, the "head" of the anterior caudate nucleus, medial amygdaloid and bed nucleus of the stria terminalis, the pyramidal layers of the hippocampus and dentate gyri, medial preoptic area, ventromedial nucleus, lateral hypothalamic and ventrolateral thalamic area, central gray, reticular part of the substantia nigra, parvoceUular reticular nucleus, Purkinje cell layer of the cerebellum, nucleus of the spinal trigeminal tract and gracile fasciculus of the spinal cord. The discrete distribution of CGRP-like binding sites in a variety of sensory systems of the brain and spinal cord as well as in thalamic and hypothalamic areas suggests a widespread involvement of CGRP in a variety of brain functions.Calcitonin gene-related peptide CGRP Autoradiography CNS ALTERNATE processing of the rat and human calcitonin genes resulted in a novel peptide, the calcitonin gene-related peptide (CGRP) [1,26,27,33]. This peptide was recently isolated and characterized [18,19]. The 37 amino acid human CGRP was found to differ from rat CGRP in four amino acids. CGRP-Iike immunoreactivity was shown to be widely distributed in the central and peripheral nervous system of man, rat and o...

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Localization of calcitonin binding sites in rat central nervous system: Evidence of its neuroactivity

Cited by 116 publications

References 21 publications

Calcitonin is expressed in gonadotropes of the anterior pituitary gland: its possible role in paracrine regulation of lactotrope function

Calcitonin is expressed in gonadotropes of the anterior pituitary gland: its possible role in paracrine regulation of lactotrope function

Amylin: Pharmacology, Physiology, and Clinical Potential

Autoradiographic distribution of 125I calcitonin gene-related peptide binding sites in the rat central nervous system

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