Localization of carbonic anhydrase in legume nodules

Atkins, Craig A.; Smith, Penelope M. C.; Mann, A.; Thumfort, Peter

doi:10.1046/j.1365-3040.2001.00680.x

Cited by 26 publications

(19 citation statements)

References 33 publications

(55 reference statements)

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“…Nodular CA activities range between 334 and 1863 µmol g −1 f. wt nodule s −1 (Atkins et al, 2001). This is much higher than the CA rates reported in the present investigation for root tissue (c. 10 µmol g −1 f. wt s −1 ).…”

Section: Discussioncontrasting

confidence: 70%

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The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

Viktor

Cramer

2004

New Phytologist

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Summary• Understanding of the influences of root-zone CO 2 concentration on nitrogen (N) metabolism is limited.• The influences of root-zone CO 2 concentration on growth, N uptake, N metabolism and the partitioning of root assimilated 14 C were determined in tomato ( Lycopersicon esculentum ).• Root, but not leaf, nitrate reductase activity was increased in plants supplied with increased root-zone CO 2 . Root phosphoenolpyruvate carboxylase activity was lower with NO 3 --than with NH 4 + -nutrition, and in the latter, was also suppressed by increased root-zone CO 2 . Increased growth rate in NO 3 --fed plants with elevated root-zone CO 2 concentrations was associated with transfer of root-derived organic acids to the shoot and conversion to carbohydrates. With NH 4 + -fed plants, growth and total N were not altered by elevated root-zone CO 2 concentrations, although 14 C partitioning to amino acid synthesis was increased.• Effects of root-zone CO 2 concentration on N uptake and metabolism over longer periods (> 1 d) were probably limited by feedback inhibition. Root-derived organic acids contributed to the carbon budget of the leaves through decarboxylation of the organic acids and photosynthetic refixation of released CO 2 .

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Section: Discussioncontrasting

confidence: 70%

“…- (Atkins et al, 2001). Nodular CA activities range between 334 and 1863 µmol g −1 f. wt nodule s −1 (Atkins et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

Viktor

Cramer

2004

New Phytologist

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“…Cytosolic and mitochondrial b-CAs are also found in C3 plants (Kachru & Anderson 1974;Reed 1979;Fett & Coleman 1994;Rumeau et al 1996;Fabre et al 2007;Tanz et al 2009;Bräutigam et al 2011) where they are believed to be involved in the replenishment of tricarboxylic acid cycle intermediates and nitrogen assimilation (Latzko & Kelly 1983;Fabre et al 2007). Cytosolic b-CAs in nitrogen-fixing nodules of legumes reportedly play different roles during development of nodules and in different nodule tissues, including supplying bicarbonate for PEPC-catalysed biosynthetic reactions, and facilitating gas exchange within the nodule and between the nodule and the rhizosphere (Gálvez et al 2000;Kavroulakis et al 2000;Atkins et al 2001;Flemetakis et al 2003).…”

Section: Plantsmentioning

confidence: 99%

Carbonic anhydrase and the molecular evolution of C₄ photosynthesis

Ludwig

2011

Plant Cell & Environment

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C4 photosynthesis, a biochemical CO2-concentrating mechanism (CCM), evolved more than 60 times within the angiosperms from C3 ancestors. The genus Flaveria, which contains species demonstrating C3, C3-C4, C4-like or C4 photosynthesis, is a model for examining the molecular evolution of the C4 pathway. Work with carbonic anhydrase (CA), and C3 and C4 Flaveria congeners has added significantly to the understanding of this process. The C4 form of CA3, a b-CA, which catalyses the first reaction in the C4 pathway by hydrating atmospheric CO2 to bicarbonate in the cytosol of mesophyll cells (mcs), evolved from a chloroplastic C3 ancestor. The molecular modifications to the ancestral CA3 gene included the loss of the sequence encoding the chloroplast transit peptide, and mutations in regulatory regions that resulted in high levels of expression in the C4 mesophyll. Analyses of the CA3 proteins and regulatory elements from Flaveria photosynthetic intermediates indicated C4 biochemistry very likely evolved in a specific, stepwise manner in this genus. The details of the mechanisms involved in the molecular evolution of other C4 plant b-CAs are unknown; however, comparative genetics indicate gene duplication and neofunctionalization played significant roles as they did in Flaveria.

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“…In an analogous but nonphotosynthetic role, bCA isoforms have been localized to nodules of legumes where they are presumed to catalyze the synthesis of HCO 3 2 for use by PEPCase in its anaplerotic role as the source of C-4 acids for amination reactions or bacteroid carbon catabolism (Flemetakis et al, 2003). In addition, the synthesis of nodule localized C-4 acids (with the coordinated expression of CA and PEPCase) has also been postulated as part of the mechanism to control gas diffusion within this tissue (Atkins et al, 2001). Other nonphotosynthetic roles for the higher plant enzyme include a requirement for a plastid localized bCA isoform identified in dark-grown cotton (Gossypium hirsutum) seedlings that appears to provide HCO 3 2 for CoA carboxylase in lipogenesis (Hoang et al, 1999).…”

mentioning

confidence: 99%

Reduction of Plastid-Localized Carbonic Anhydrase Activity Results in Reduced Arabidopsis Seedling Survivorship

2008

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Carbonic anhydrase (CA; EC 4.2.1.1) catalyzes the interconversion of CO2 and HCO3 − and is a major protein constituent of the C3 higher plant chloroplast where it is presumed to play a role in photosynthetic carbon assimilation. In this study, we have used both RNA antisense and gene knockout lines to specifically reduce the activity of the chloroplast βCA1 polypeptide (At3g01500) in the model plant Arabidopsis (Arabidopsis thaliana). Although able to germinate, seedling establishment of transgenic plants is significantly reduced relative to wild-type plants when grown at ambient levels of CO2. Growth at elevated (1,500 μL L−1) CO2 or on plates supplemented with sucrose restores seedling establishment rates to wild-type levels. Seed from wild-type and transgenic plants exhibited no significant differences in seed protein, lipid content, or reserve mobilization during seedling growth. βCA1-deficient seedlings do, however, exhibit reduced capacity for light-dependent 14CO2 assimilation prior to the development of true leaves. The small number of surviving seedlings able to grow and develop are phenotypically similar to wild-type plants, even when subsequently grown at subambient levels of CO2. Microarray analysis of mature leaves of βCA1-deficient plants shows some differences in transcript abundance, particularly with genes involved in ethylene signaling and response. The data suggest that reduced levels of seedling establishment by βCA1-deficient plants could be the result of poor cotyledon photosynthetic performance at the onset of phototrophic growth and prior to the development of true leaves.

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Localization of carbonic anhydrase in legume nodules

Cited by 26 publications

References 33 publications

The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

Carbonic anhydrase and the molecular evolution of C₄ photosynthesis

Reduction of Plastid-Localized Carbonic Anhydrase Activity Results in Reduced Arabidopsis Seedling Survivorship

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Localization of carbonic anhydrase in legume nodules

Cited by 26 publications

References 33 publications

The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning

Carbonic anhydrase and the molecular evolution of C4 photosynthesis

Reduction of Plastid-Localized Carbonic Anhydrase Activity Results in Reduced Arabidopsis Seedling Survivorship

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Carbonic anhydrase and the molecular evolution of C₄ photosynthesis