Localization of cholecystokinin in the zebrafish retina from larval to adult stage

Guerrera, Maria Cristina; Abbate, Francesco; Di, Gianfranco; Germanà, G; Levanti, Maria; Micale, V.; Montalbano, Giuseppe; Laurà, Rosaria; Germanà, Antonino; Muglia, U.

doi:10.1016/j.aanat.2018.01.010

Cited by 3 publications

(2 citation statements)

References 69 publications

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“…Putative cholinergic DACs were found in the zebrafish, Danio rerio , Pacific Coast dogfish, Squalus acanthias , and goldfish (Brandon, 1991; Paulsen et al, 2010; Tumosa & Stell, 1986). Cholecystokinin‐immunoreactive cells occur in the zebrafish (Guerrera et al, 2018). Putative GABAergic DACs were revealed in the goldfish, channel catfish, Ictalurus punctatus , and Atlantic salmon, Salmo salar (Brandon, 1985; Ekström & Anzelius, 1998; Pow et al, 1996; Yazulla et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

Retinal ganglion cell topography and spatial resolution in the Japanese smelt Hypomesus nipponensis (McAllister, 1963)

2020

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Vision plays a crucial role in the life of the vast majority of vertebrate species. The spatial arrangement of retinal ganglion cells has been reported to be related to a species’ visual behavior. There are many studies focusing on the ganglion cell topography in bony fish species. However, there are still large gaps in our knowledge on the subject. We studied the topography of retinal ganglion cells (GCs) in the Japanese smelt Hypomesus nipponensis, a highly visual teleostean fish with a complex life cycle. DAPI labeling was used to visualize cell nuclei in the ganglion cell and inner plexiform layers. The ganglion cell layer was relatively thin (about 6‐8 μm), even in areas of increased cell density (area retinae temporalis), and was normally composed of a single layer of cells. In all retinal regions, rare cells occurred in the inner plexiform layer. Nissl‐stained retinae were used to estimate the proportion of displaced amacrine cells and glia in different retinal regions. In all retinal regions, about 84.5% of cells in the GC layer were found to be ganglion cells. The density of GCs varied across the retina in a regular way. It was minimum (3990 and 2380 cells/mm2 in the smaller and larger fish, respectively) in the dorsal and ventral periphery. It gradually increased centripetally and reached a maximum of 14,275 and 10,960 cells/mm2 (in the smaller and larger fish, respectively) in the temporal retina, where a pronounced area retinae temporalis was detected. The total number of GCs varied from 177 × 103 (smaller fish) to 212 × 103 cells (larger fish). The theoretical anatomical spatial resolution (the anatomical estimate of the upper limit of visual acuity calculated from the density of GCs and eye geometry and expressed in cycles per degree) was minimum in the ventral periphery (smaller fish, 1.46 cpd; larger fish, 1.26 cpd) and maximum in area retinae temporalis (smaller fish, 2.83 cpd; larger fish, 2.75 cpd). The relatively high density of GCs and the presence of area retinae temporalis in the Japanese smelt are consistent with its highly visual behavior. The present findings contribute to our understanding of the factors affecting the topography of retinal ganglion cells and visual acuity in fish.

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Section: Discussionmentioning

confidence: 99%

Retinal ganglion cell topography and spatial resolution in the Japanese smelt Hypomesus nipponensis (McAllister, 1963)

2020

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“…The exact function of DACs in fish is unknown. There are several types of DACs in fish differing in neurochemical profile (e.g., Bonci et al, 2006; Ekström & Anzelius, 1998; Guerrera et al, 2018; Paulsen et al, 2010; Yazulla & Studholme, 1990) suggesting their functional diversity and various roles in signal processing in the GCL. In mammals, DACs contribute to GC functioning, and in particular, complicate GC‐mediated signal processing (Heukamp et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Retinal ganglion cell topography and spatial resolving power in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856)

Pushchin,

Aleskerov

2024

Journal of Fish Biology

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We studied the topography of retinal ganglion cells (GCs) and estimated spatial resolving power (SRP) in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856), a relatively small brightly colored fish inhabiting coral reefs and lagoons in the Western Pacific. S. nematoptera is an active night predator feeding on near‐bottom animal plankton and benthos. DAPI staining was used to label nuclei of GCs and non‐GCs in the inner plexiform and ganglion cell layers. Non‐GCs were distinguished from GCs in Nissl‐stained retinal wholemounts based on cell size, shape, and staining intensity. The proportion of displaced amacrine cells (DACs) varied from 15.46 ± 1.12 (visual streak [VS]) to 17.99 ± 1.06% (dorsal periphery) (mean ± S.E.M., N = 5); the respective proportions of glial cells were 6.61 ± 0.84 and 5.89 ± 0.76%. Thus, 76%–78% of cells in the ganglion cell layer and inner plexiform layer were GCs. The minimum spatial coverage of GCs (3600–4600 cells/mm2) was detected in the dorsal and ventral periphery. It gradually increased toward the central retina to form a moderate VS. The maximum GC density (11,400–12,400 cells/mm2) was registered in the central portion of the VS. No pronounced concentric retinal specializations were found. The total number of GCs ranged within 595.2–635.9 × 103. The anatomical spatial resolving power was minimum in the ventral periphery (4.91–5.53 cpd) and maximum in the central portion of the VS (8.47–9.07 cpd). The respective minimum separable angles were 0.18–0.20° and 0.11–0.12°. The relatively high spatial resolving power and presence of the VS in the pajama cardinalfish are in line with its highly visual behavior.

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Effect of intracerebroventricular administration of two molecular forms of sulfated CCK octapeptide on anxiety-like behavior in the zebrafish danio rerio

et al. 2020

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Localization of cholecystokinin in the zebrafish retina from larval to adult stage

Cited by 3 publications

References 69 publications

Retinal ganglion cell topography and spatial resolution in the Japanese smelt Hypomesus nipponensis (McAllister, 1963)

Retinal ganglion cell topography and spatial resolution in the Japanese smelt Hypomesus nipponensis (McAllister, 1963)

Retinal ganglion cell topography and spatial resolving power in the pajama cardinalfish Sphaeramia nematoptera (Bleeker, 1856)

Effect of intracerebroventricular administration of two molecular forms of sulfated CCK octapeptide on anxiety-like behavior in the zebrafish danio rerio

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