ObjectiveA tumor model in the rat was used to study peritoneal tumor growth and abdominal wall metastases after carbon dioxide (002) pneumoperitoneum, gasless laparoscopy, and laparotomy.
Summary Background DataThe role of laparoscopic resection of cancer is under debate. Insufflation of the peritoneal cavity with C02 is believed to be a causative factor in the development of abdominal wall metastases after laparoscopic resection of malignant tumors.
MethodsIn the solid tumor model, a lump of 350-mg CC-531 tumor cells was placed intraperitoneally in rats having C02 pneumoperitoneum (n = 8), gasless laparoscopy (n = 8), or conventional laparotomy (n = 8). After 20 minutes, the solid tumor was removed through a laparoscopic port or through the laparotomy. In the cell seeding model, 5 x 105 CC-531 cells were injected intraperitoneally before C02 pneumoperitoneum (n = 12), gasless laparoscopy (n = 12), or laparotomy (n = 12). All operative procedures lasted 20 minutes. After 6 weeks, in the solid tumor model and after 4 weeks in the cell seeding model, tumor growth was scored semiquantitatively. All results were analyzed using the analysis of variance.