Localization of Connexin 26, 32 and 43 in Rat Parotid Glands.

Lee, Chan Young; Muramatsu, Takashi; Shimono, Masaki

doi:10.1267/ahc.31.211

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…(1994) reported that Cx32 and Cx26 in rat salivary glands were colocalized within same GJ plaques). Lee et al . (1998) suggested that Cx26 and 32 were associated with secretary function and permeability.…”

Section: Discussionmentioning

confidence: 99%

Morphological and functional changes of the rat parotid glandular cells by clipping and reopening the parotid duct, using HAM8 antibody

et al. 2008

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The purpose of this experiment is to examine the proliferative process of rat acinar cells after parotid duct ligation and reopening. Two experimental groups were observed. The first group was killed from 0 to 14 days after the duct ligation. In the second group, the duct was clipped for 14 days, and it was reopened. Following a period of from 2 to 28 days after removal of the clip, the glands were removed to perform a histological analysis, including hematoxylin-eosin (HE), immunofluorescent staining using HAM8 antibody, which recognizes connexin 32, and transmission electron microscopy (TEM). In the experimental gland from the 1st group at 6 days after ligation (I-6D), the acinar cells disappeared. In the tissue from the 2nd group 8 days after reopening (II-8D), newly formed acinar cells were found again. Lobular structure of the parotid glands recovered in the II-21D. HAM8 signals were observed between normal acinar cells, while they declined in the tissue from I-1D, and they were not observed in the I-2D. HAM8 signals were first observed in the II-25D and then subsequently returned to normal levels in the II-28D. These results suggest that the intercellular communication and functional recovery was not complete 25 days after reopening of the duct.In conclusion, the recovery of the acinar structure was recognized during an extended period of duct ligation, however, a time lag between the morphological and functional recovery was found to exist.

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Section: Discussionmentioning

confidence: 99%

Morphological and functional changes of the rat parotid glandular cells by clipping and reopening the parotid duct, using HAM8 antibody

et al. 2008

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“…Right: magnitude of the coupling of control (Con) and male Cx32-deficient animals was similar (10.0 Ϯ 2.5 and 10.6 Ϯ 2.5 ps, respectively) whereas that of the few cell pairs that were coupled from the Cx32-deficient female glands had a lower conductance (1.8 Ϯ 1.2 ps). *P Ͻ 0.05. via a protein kinase C system (8,9). Although it is clear that gap junctions are present and can be regulated, there are no data on either mouse or rat lacrimal or salivary gland systems to show the role of gap junctions in the secretory process itself.…”

Section: Discussionmentioning

confidence: 99%

Role of gap junctions in fluid secretion of lacrimal glands

Walcott

Moore

Birzgalis

et al. 2002

American Journal of Physiology-Cell Physiology

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In glands such as the liver and pancreas, gap junctions containing connexin 26 and 32 (Cx26 and Cx32, respectively) couple the secretory cells. Uncoupling these junctions compromises the secretory function of these glands. Lacrimal glands also contain extensive arrays of gap junctions consisting of Cx26 and Cx32. We wanted to determine the role of these junctions in fluid secretion. In Cx32-deficient mice, immunocytochemistry showed that, in the male lacrimal gland, the remaining Cx26 was found evenly distributed in the membrane whereas there was little in the membranes of female glands. Western blot analysis of Cx26 showed that female Cx32-deficient mice expressed Cx26. Patch-clamp analyses of acinar cell coupling showed that the cell pairs from male glands were coupled whereas those from female glands were not. Stimulated fluid production by the glands from Cx32-deficient mice was abnormally low in female glands compared with controls at low topical doses of carbachol. The protein secretory response to different doses of carbachol was the same in all animals. These data suggest that gap junctions are essential for optimal fluid secretion in lacrimal glands.

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“…12). They have not been documented in SDs either by freeze‐fracture (Shimono et al, 1980) or by immunocytochemistry (Hirono et al, 1995; Lee et al, 1998). Recently, however, Iwasa and Kondo (1999) have reported the presence of many point contacts (focal gap junctions?)…”

Section: Innervationmentioning

confidence: 99%

Secretion by striated ducts of mammalian major salivary glands: Review from an ultrastructural, functional, and evolutionary perspective

et al. 2001

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In addition to their role in electrolyte homeostasis, striated ducts (SDs) in the major salivary glands of many mammalian species engage in secretion of organic products. This phenomenon usually is manifested as the presence of small serous-like secretory granules in the apical cytoplasm of SD cells. The composition of these granules is largely unknown, except in the case of the cat and rat submandibular gland, where the granules have unequivocally been shown to contain kallikrein. In some species, the apical cytoplasm of SD cells contains variable numbers of vesicles, both spherical and elongated, that vary in appearance from 'empty' to moderately dense. In the rat parotid gland, lucent vesicles transport glycoproteins to the luminal surface where they are incorporated into the apical plasmalemma and the glycocalyx. There is a strong possibility that in various species some of these vesicles are involved in transcytosis of antibodies to the saliva from their source (plasma cells) in the surrounding connective tissue. In addition, vesicles may engage in transfer of growth factors from the saliva to the interstitium. In a few species, conventional SDs have been replaced by ducts that are wholly given over to secretion, i.e., they entirely lack basal striations; although such ducts occupy the histological position of conventional SDs, it is not clear whether they represent a new type of duct or merely are modifications of SDs. Broad-based comparisons of ultrastructural and other data about SDs offer some insight into evolutionary history of salivary glands and their role in the adaptive radiation of mammals. Evolutionary patterns emerged when we made interspecific comparisons across mammalian orders. Among the bats, there is a clear relationship between SD secretion and general categories of diet. Anat Rec 264: 2001.

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Localization of Connexin 26, 32 and 43 in Rat Parotid Glands.

Cited by 9 publications

References 24 publications

Morphological and functional changes of the rat parotid glandular cells by clipping and reopening the parotid duct, using HAM8 antibody

Morphological and functional changes of the rat parotid glandular cells by clipping and reopening the parotid duct, using HAM8 antibody

Role of gap junctions in fluid secretion of lacrimal glands

Secretion by striated ducts of mammalian major salivary glands: Review from an ultrastructural, functional, and evolutionary perspective

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