Localization of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry.

Koji, Takehiko; Komuta, Ko; Nozawa, Michiyo; Yamada, Shinkichi; Nakane, Paul K.

doi:10.1177/42.10.7930523

Cited by 28 publications

(26 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In SWH, however, NF-kB was only detected when it was unbound or weakly bound to the response element. Because these methods provide complementary results to each other, they can be used to characterize the physiological state of NF-kB [9]. In our study, there was no correlation between SWH and IHC, which can be explained by the fact that IHC and SWH detect different forms of NF-kB.…”

Section: Discussioncontrasting

confidence: 41%

NF-kB Expression in IgA Nephropathy Outcome

et al. 2011

View full text Add to dashboard Cite

Abstract. Some studies have demonstrated the involvement of nuclear factor-kappa B (NF-kB) in the pathogenesis of glomerulonephritis. The aim of our study was twofold: (1) to analyze the prognostic value of NF-kB expression in primary IgA nephropathy (IgAN) and (2) to compare the results of NF-kB expression by immunohistochemistry (IHC) and southwestern histochemistry (SWH). We analyzed 62 patients diagnosed with IgAN from 1987 to 2003. We used monoclonal antibodies to CD68 and mast cell tryptase and polyclonal antibodies to TGF-β1, α-SMA and NF-kB p65. We used SWH for the in situ detection of activated NF-kB. The results showed that NF-kB expression (mainly by SWH) correlated with clinical and histological parameters. An unfavorable clinical course of IgAN was significantly related to tubular NF-kB expression by SWH, but not by IHC. The KaplanMeier curves demonstrated that increased NF-kB expression, which was measured by IHC and SWH, decreased renal survival. In conclusion, the increased expression of NF-kB in the tubular area may be a predictive factor for the poor prognosis of patients with IgAN. Compared with IHC, NF-kB expression determined by SWH was correlated with a larger number of parameters of poor disease outcome.

show abstract

Section: Discussioncontrasting

confidence: 41%

NF-kB Expression in IgA Nephropathy Outcome

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The method is based on a principle that transcriptional factors recognize specific DNA sequences and bind to them [6][7][8]10]. Since antibodies against ETF and GCF are not currently available, southwestern histochemistry can be considered as only one method to offer a useful information on the expression of these transcriptional factors in individual cells.…”

Section: Discussionmentioning

confidence: 99%

“…Very recently, we have developed a method to localize specific DNA binding proteins using haptenized double-stranded (ds) DNAs containing specific consensus base sequences [6-8 , 267 Correspondence to: Dr Ko Komuta1 and Dr. Takehiko Koji2, [6,10].…”

mentioning

confidence: 99%

Localization of Epidermal Growth Factor Receptor Enhancer Protein in A431 Epidermoid Carcinoma Cells by Southwestern Histochemistry.

Komuta

Kanematsu

Nakane

et al. 1998

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…Southwestern histochemistry was performed as described previously [25][26][27]. In brief, deparaffinized sections (4-m-thick) were treated in a microwave oven with citrate buffer (pH 6.0) at 95°C for 15 min.…”

Section: Southwestern Histochemistry For Nf-bmentioning

confidence: 99%

“…Therefore, we investigated the expression of activated NF-B in our model. Southwestern histochemistry is used to detect activated transcription regulatory factors that bind to specific sequences of DNA [25,26].…”

Section: Southwestern Histochemistry For Nf-bmentioning

confidence: 99%

Epigallocatechin gallate suppresses peritoneal fibrosis in mice

Kitamura

Nishino

Obata

et al. 2012

Chemico-Biological Interactions

View full text Add to dashboard Cite

Long-term peritoneal dialysis (PD) leads to histological changes in the peritoneal membrane.Angiogenesis and inflammation caused by glucose degradation products (GDPs) play crucial roles in peritoneal fibrosis. One such GDP is methylglyoxal (MGO), which enhances the formation of advanced glycation end products (AGEs). AGEs bind to their receptor (RAGE) and activate nuclear factor-B (NF-B), which is a key regulator of angiogenesis and inflammation. Recent studies have indicated that (-)-epigallocatechin gallate (EGCG), a tea polyphenol, inhibits angiogenesis and inflammation. Here, we examined whether EGCG suppresses peritoneal fibrosis in mice.Based on preliminary examination, 2 mL of 40 mM MGO or PD fluid was injected intraperitoneally and EGCG (50 mg/kg) or saline was injected subcutaneously for 3 weeks.In comparison to PD fluid + saline-treated mice, the peritoneal tissues of MGO + salinetreated mice showed marked thickening of the submesothelial compact zone. In the submesothelial compact zone of the MGO + saline-treated mice, CD31-positive vessels and vascular endothelial growth factor-positive cells were significantly increased, as were inflammation, F4/80-positive macrophages, and monocyte chemotactic protein-1. Moreover, 8-hydroxydeoxyguanosine, a marker of reactive oxygen species, and NF-B, determined by 3 Southwestern histochemistry, in the submesothelial compact zone were also increased in MGO + saline-treated mice. These changes were attenuated in MGO + EGCG-treated mice.We demonstrated that EGCG treatment suppresses peritoneal fibrosis via inhibition of NF-B. Furthermore, EGCG inhibits reactive oxygen species production. The results of this study indicate that EGCG is a potentially novel candidate for the treatment of peritoneal fibrosis.

show abstract

Localization of cyclic adenosine 3',5'-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry.

Cited by 28 publications

References 4 publications

NF-kB Expression in IgA Nephropathy Outcome

NF-kB Expression in IgA Nephropathy Outcome

Localization of Epidermal Growth Factor Receptor Enhancer Protein in A431 Epidermoid Carcinoma Cells by Southwestern Histochemistry.

Epigallocatechin gallate suppresses peritoneal fibrosis in mice

Contact Info

Product

Resources

About