Localization of dopamine D1A receptor protein in rat kidneys

Abstract

The dopamine D1A receptor subtype was identified in rat kidney with both light microscopic immunohistochemistry and electron microscopic immunocytochemistry. Antipeptide polyclonal antisera were directed to both extracellular and intracellular regions of the native receptor. The use of such receptor-subtype-selective antibodies allows for the identification of specific dopamine receptor subtype clones that are not distinguished by current pharmacological or receptor-ligand binding technology. Selectivity of the antipeptide antisera was validated by their ability to recognize native receptor protein expressed in permanently transfected mouse LTK- cells. In the rat kidney, D1A receptor protein was localized to the juxtaglomerular apparatus (JGA), proximal tubule, distal tubule, cortical collecting duct, and renal vasculature. In the JGA, the receptor was predominantly located in the arteriolar smooth muscle layer within cytoplasmic granules previously shown to contain renin. In the proximal tubules, staining was localized both on the brush-border and basolateral membranes. The D1A receptor, which is present in the central nervous system, is now identified in the rat kidney at those sites previously labeled as DA1 receptor sites on the basis of pharmacological binding studies. These results suggest that at least some of the renal dopamine DA1 receptors correspond structurally to the central dopamine D1A receptor.

Blood Pressure and Renal Sodium Handling in Relation to Genetic Variation in the DRD1 Promoter and GRK4

“…19,20 However, the kidney expresses DRD1 not only in the proximal tubules, but also in the medullary ascending limb of Henle, and in the medullary and cortical collecting ducts. 21,22 In the current study population, we previously demonstrated an inverse relation between RNa prox and mean arterial pressure. 9 In line with these findings, 9 the current analyses of the DRD1 haplotypes H1-AGC and H3-AAC showed opposite trends in blood pressure and RNA prox .…”

Rat Strain Effects of AT 1 Receptor Activation on D 1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells

“…The specificity of this D 1 receptor antibody has also been reported. 19,29 Rat RPT cells were treated with vehicle (dH 2 O), angiotensin II or an AT 1 receptor antagonist (losartan) at the indicated concentrations and times. Immunoblotting was performed as previously reported, 22,26 except that the transblots were probed with the D 1 (1:800) or the AT 1 receptor antibody (1:400).…”

Dopamine, hypertension and obesity

“…10 D 2 -like receptors are also localised in the juxtaglomerular apparatus, the proximal and distal cortical tubules and CCD, in the renal vasculature adventitia, in the glomerulus and the terminal of the sympathetic nerves. [10][11][12] …”