Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes

Terasaki, Mark; Song, Jiuzhou; Wong, James R.; Weiss, Michael J.; Chen, Lan Bo

doi:10.1016/0092-8674(84)90530-0

Cited by 371 publications

(238 citation statements)

References 10 publications

Supporting

Mentioning

223

Contrasting

Unclassified

Order By: Relevance

“…The ER and the Golgi apparatus are closely linked not only by their location in the perinuclear area of the cytoplasm, but also as they interact together in the case of newly synthesised proteins. The ER is known to play a central role in the biosynthesis, segregation and transport of proteins and lipids as well as in the release of intracellular stores of calcium (Terasaki et al, 1984). The Golgi apparatus receives newly synthesised proteins from the ER and modifies them chemically, for example, by glycosylation or sulphonation (Short and Barr, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…To visualise the Golgi apparatus, cells were labelled with 4 mm BPC for 15 min (Pagano et al, 1991). The ER was labeled with the lipophilic, cationic DiOC 6 dye applied for 15 min at a final concentration of 2 mg ml À1 (Terasaki et al, 1984). This marker, used in the concentration range 1 -10 mg ml À1 , was reported to be highly specific for ER and, compared to other possible candidates, is brightest with the slowest bleaching rate (Sabnis et al, 1997).…”

Section: Treatment Of Cells Prior To Microspectrofluorometry and Confmentioning

confidence: 99%

See 1 more Smart Citation

Endoplasmic reticulum and Golgi apparatus are the preferential sites of Foscan® localisation in cultured tumour cells

et al. 2003

View full text Add to dashboard Cite

Intracellular photosensitiser localisation significantly influences the mechanism of response to photodynamic therapy (PDT), since the primary sites of damage are closely related to the specific sensitiser distribution. Foscan s subcellular localisation in the MCF-7 human adenocarcinoma cell line has been studied by means of confocal microscopy and microspectrofluorometry. The fluorescence topographic profiles recorded after cells costained with Foscan s and organelle-specific fluorescent probes revealed that Foscan s presents low localisation in lysosomes and a weak accumulation in mitochondria. Alternatively, the Foscan s fluorescence topographic profile turned out to colocalise perfectly with that obtained for the endoplasmic reticulum (ER) and the Golgi apparatus. The patterns of fluorescence derived from confocal microscopy studies were consistent with predominant localisation of Foscan s in these organelles. Furthermore, evaluation of enzymatic activity of selected organelles immediately after laser light irradiation (650 nm) indicated the Golgi apparatus and ER as the primary damaged sites resulting from Foscan s -mediated PDT in the MCF-7 cell line. To our knowledge, this is the first study to demonstrate unambiguously that the ER and the Golgi apparatus are preferential sites of Foscan s accumulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Treatment Of Cells Prior To Microspectrofluorometry and Confmentioning

confidence: 99%

Endoplasmic reticulum and Golgi apparatus are the preferential sites of Foscan® localisation in cultured tumour cells

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The stain has been shown to mark the location of endoplasmic reticulum and Golgi membranes in living cells [21]. This stain clearly marked the central juxtanuclear site (Fig.…”

Section: Location Of Ca 2ϩ Storage Sites In Neutrophilsmentioning

confidence: 99%

Two distinct Ca2+ storage and release sites in human neutrophils

Pettit

Hallett

1998

Journal of Leukocyte Biology

View full text Add to dashboard Cite

in response to N-formyl-methionyl-leucyl-phenylalanine, whereas engagement and clustering of CD11b/CD18 integrins causes Ca 2؉ release from the peripheral stores. The release sites also correlated with organelles that stained with DiOC 6 (3). Localized phototoxicity generated by DiOC 6 (3) excitation resulted in inhibition of the release of stored Ca 2؉ , which was selective for the stimulus used. The presence of two distinct cellular locations for these Ca 2؉ stores and their independent release raises the possibility that separate intracellular messengers for their release are generated.

show abstract

“…This type of ER is generally organized in sheets (cisternae) or in a branching tubular network typically seen in many cultured mammalian cells. Such networks are characterized by fairly straight tubules, which branch at tripartite junctions to generate a polygonal meshwork [5,9]. Only in some cells (e.g.…”

Section: Introductionmentioning

confidence: 99%

Endoplasmic reticulum architecture: structures in flux

Borgese

Francolini

Snapp

2006

Current Opinion in Cell Biology

206

147

View full text Add to dashboard Cite

The endoplasmic reticulum (ER) is a dynamic pleiomorphic organelle containing continuous but distinct subdomains. The diversity of ER structures parallels its many functions, including secretory protein biogenesis, lipid synthesis, drug metabolism and Ca 2+ signaling. Recent studies are revealing how elaborate ER structures arise in response to subtle changes in protein levels, dynamics, and interactions as well as in response to alterations in cytosolic ion concentrations. Subdomain formation appears to be governed by principles of self-organization. Once formed, ER subdomains remain malleable and can be rapidly transformed into alternative structures in response to altered conditions. The mechanisms that modulate ER structure are likely to be important for the generation of the characteristic shapes of other organelles.

show abstract

Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes

Cited by 371 publications

References 10 publications

Endoplasmic reticulum and Golgi apparatus are the preferential sites of Foscan® localisation in cultured tumour cells

Endoplasmic reticulum and Golgi apparatus are the preferential sites of Foscan® localisation in cultured tumour cells

Two distinct Ca2+ storage and release sites in human neutrophils

Endoplasmic reticulum architecture: structures in flux

Contact Info

Product

Resources

About