Localization of estrogen, epidermal growth factor, and transferrin receptors in human prostatic carcinoma.

Moriyama, Nobuo; Nagase, Yasushi; Doi, Naoto; Higashihara, Eiji; Murahashi, Isao; Aso, Yoshio

doi:10.1267/ahc.24.61

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…The study procedure was similar to that used previously [ 8, 9]. The following antibodies were used in the present series: antihuman Fas mouse IgG monoclonal antibody, UB2 (MBL, Tokyo, Japan), anti‐Fas ligand rabbit polyclonal antibody (MBL) and antihuman ICE rabbit IgG antibody (p20 subunits, Upstate Biotechnology, Lake Placid, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical study of Fas, Fas ligand and interleukin‐1β converting enzyme expression in human prostatic cancer

Sasaki

Ahmed

Takéuchi

et al. 1998

British Journal of Urology

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Objective To determine the cellular expression of Fas, Fas ligand and interleukin‐1β converting enzyme (ICE) in prostatic cancer. Patients and methods Specimens of prostate were obtained from 21 patients (mean age 66 years, sd 5) undergoing radical prostatectomy for prostatic cancer. Nine of the 21 patients had received endocrine therapy before surgery. Specimens were also obtained from 10 patients with benign prostatic hypertrophy (BPH) and during autopsy from 10 patients who had died from hormone‐unresponsive prostate cancer. Paraffin‐embedded sections were cut from the specimens and stained immunohistochemically to detect Fas, Fas ligand and ICE. Results Fas was expressed in all 21 of the cancer specimens while Fas ligand was detected in none and ICE was expressed in 11. The difference between the expression of Fas and ICE was significant (P<0.001). ICE was expressed in nine of 12 patients who were untreated before surgery and in two of nine treated with endocrine therapy (P<0.05). Fas expression was detected in the specimens from all 10 patients with BPH and in all 10 autopsy specimens; the expression tended to be more marked than that in specimens from total prostatectomy (P<0.1). Conclusion The expression of ICE was weaker than that of Fas in the total prostatectomy specimens, suggesting a possible interruption of the apoptotic signalling pathway. Prostates with BPH and hormone‐unresponsive cancer therefore showed no appreciable change in Fas expression when compared with total prostatectomy samples.

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Section: Methodsmentioning

confidence: 99%

Immunohistochemical study of Fas, Fas ligand and interleukin‐1β converting enzyme expression in human prostatic cancer

Sasaki

Ahmed

Takéuchi

et al. 1998

British Journal of Urology

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“…Oncogene products, such as ras-p21 or the epidermal growth factor receptor (EGFR), were studied as new markers of the behavior of prostate and bladder tumors (20,24,36,37).…”

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confidence: 99%

“…The acid phosphatase activity of prostate cancer is markedly lowered by hormonal treatment (2, 14), and the same is true for androgen receptors (14). Oncogene products such as the epidermal growth factor receptor and erb-B2 are also expressed by various urological malignancies (21,38 3. Review of the studies performed at our department. 1) Histocytochemistry of the prostate Use of histocytochemistry in the urological field commenced with the study of prostatic acid phosphatase by Gomori's method (13).…”

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confidence: 99%