Localization of frog retinal pigment epithelium Na+-K+ ATPase

Ostwald, Thomas J.; Steinberg, Roy H.

doi:10.1016/s0014-4835(80)80043-1

Cited by 59 publications

(28 citation statements)

References 26 publications

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“…In the RPEcells, however, Na, K-ATPase is mainly located on the apical plasma membranes (17,18,20,27). Okami et al (17) showed by quantitative immunogoldelectron microscopy that approximately 85% of Na, K-ATPase was distributed on the apical surface of the adult rat RPEcells.…”

Section: Discussionmentioning

confidence: 99%

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Immunocytochemical Analyses of Distributions of Na, K-ATPase and GLUT1, Insulin and Transferrin Receptors in the Developing Retinal Pigment Epithelial Cells.

Sugasawa

Deguchi

Okami

et al. 1994

Cell Struct. Funct.

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Key words: retinal pigment epithelium/immunocytochemistry/Na, K-ATPase/glucose transporter/insulin receptor/transferrin receptor ABSTRACT. The retinal pigment epithelium (RPE) is unique in that Na, K-ATPase is predominantly localized on its apical surface. Westudied the distributions of Na, K-ATPase and glucose transporter GLUT1,insulin and transferrin receptors in developing rat RPE cells immunocytochemically. Na, K-ATPase, first detected in 17-day-old embryonic eyes, was already distributed predominantly on the apical surface. This reversed distribution of Na, K-ATPasewas maintained throughout their life. Insulin receptor and transferrin receptor were distributed exclusively on the basolateral surface. By quantitative immunogold electron microscopic technique we found that glucose transporter GLUT1is distributed almost equal in amount on both the apical and basolateral surfaces of RPEcells, thus presumably constructing an efficient pathway for glucose transport from the choriocapillaries to the neural retina through the blood-retinal barrier. These results suggest that in the RPE cells the intrinsic basolateral plasma membrane proteins are sorted out at least in three different ways.

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Section: Discussionmentioning

confidence: 99%

“…Na, K-ATPase is located usually on the basolateral plasma membrane (21). Contradictory to the general rule, Na, K-ATPase in the RPE cells of rat is distributed predominantly on the apical surfaces (17,18,27). In chicken, apical distribution of Na, K-ATPase is already established at the embryonic stage (20).…”

mentioning

confidence: 99%

Immunocytochemical Analyses of Distributions of Na, K-ATPase and GLUT1, Insulin and Transferrin Receptors in the Developing Retinal Pigment Epithelial Cells.

Sugasawa

Deguchi

Okami

et al. 1994

Cell Struct. Funct.

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“…The apical distribution of Na+/K+-ATPase on polarized RPE cells (Gundersen et al, 1991;Ostwald & Steinberg, 1980), which is opposite to its basolateral distribution in other polarized cell types, fails to change the transport and distribution of VSV gG and influenza virus HA in polarized RPE cells (Bok et al, 1992). To study the distribution of HCMV gB on the membranes of polarized RPE cells, the cells were grown on filters, infected from the apical surface with strain AD169 (1-3 p.f.u./cell) and fixed at 6 days post-infection as described in Methods.…”

Section: Hcmv Gb Is Transported To the Apical Membrane Of Infected Rpmentioning

confidence: 99%

“…The tight junctions between cells of the RPE form the barrier between the choroidal blood supply and the retina. The Na+/K+-ATPase and the associated ankyrin-fodrin submembrane cytoskeleton are in the apical membrane of polarized RPE cells, the reverse of their position in other polarized cells (Gundersen et al, 1991;Miller et al, 1978;Ostwald & Steinberg, 1980). It was reported that RPE cells cultured from patients with HCMV retinitis transmit infection to RPE cells from normal donors, which demonstrates that cultured human RPE cells are fully permissive for HCMV replication (Miceli et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells

Tugizov

Maidji

Pereira

1996

Journal of General Virology

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Human retinal pigment epithelial (RPE) cells, which are permissive for human cytomegalovirus (HCMV) replication, were used to evaluate virus infection from apical and basolateral membranes of polarized cells. Tests of HCMV infectivity showed that the apical membrane was 20-30-fold more susceptible to infection than the basolateral membrane; in contrast, both membranes were equally susceptible to infection by herpes simplex virus type 1 (HSV-1). Neutralizing monoclonal antibodies (MAbs) to HCMV glycoprotein B (gB) blocked penetration of virions into polarized RPE cells. This indicated that gB has a function in fusion of the virion envelope with the apical membrane of these cells, as it has with the cell membrane of unpolarized human fibroblasts. In contrast to HSV-l-infected RPE cells, the paracellular permeability of polarized RPE cells changed slowly following infection with HCMV. Confocal microscopy examination of HCMV-infected RPE cells revealed that the pattern of ZO-1 staining was altered at late times. Addition ofgB-specific neutralizing MAbs to the apical and basolateral membranes of HCMV-infected RPE cells failed to inhibit plaque development; this indicated that progeny virions infect adjacent cells before disassembly of tight junctions and are sequestered from neutralization during spread across lateral cell membranes. The finding that progeny HCMV virions cross lateral cell membranes, which differ substantially in protein composition from apical membranes, suggests that polarized RPE cells contain multiple receptors for HCMV.

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“…Extracellular matrix, filling subretinal space is medium for molecular interactions between choroid, RPE and sensory retina. RPE cells' membrane with Na+ and K+ ATPase affects inflow and outflow of ions in this matrix (Ostwald & Steinberg, 1980) and by this ionic transferring, initial conversion of light energy to neuronal impulses occurs. RPE also has an important role in absorption of scattered lights entering eyes which can interfere visual function.…”

Section: Electroretinographymentioning

confidence: 99%

Diagnostic Values of Electrophysiology in Ophthalmology

Movassat¹

2012

Electrophysiology - From Plants to Heart

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Localization of frog retinal pigment epithelium Na+-K+ ATPase

Cited by 59 publications

References 26 publications

Immunocytochemical Analyses of Distributions of Na, K-ATPase and GLUT1, Insulin and Transferrin Receptors in the Developing Retinal Pigment Epithelial Cells.

Immunocytochemical Analyses of Distributions of Na, K-ATPase and GLUT1, Insulin and Transferrin Receptors in the Developing Retinal Pigment Epithelial Cells.

Role of apical and basolateral membranes in replication of human cytomegalovirus in polarized retinal pigment epithelial cells

Diagnostic Values of Electrophysiology in Ophthalmology

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