Localization of gender-regulated gene expression in the filarial nematode Brugia malayi

Jiang, Daojun; Li, Ben-Wen; Fischer, Peter; Weil, Gary J.

doi:10.1016/j.ijpara.2007.09.010

Cited by 25 publications

(34 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tissue transcriptional studies of some important genes have been reported in A. suum, H. contortus and B. malayi, which have provided valuable information regarding tissue-specific genes (Yin et al, 2008;Wang et al, 2013;Rosa et al, 2014). For example, Bm-shp-1 expression in the epithelium of uterine and Bm-msp-1 in spermatocytes and spermatids indicated potential roles in reproductive processes (Jiang et al, 2008). Similarly, in our study, differential expression analysis among tissues revealed consistent transcription profiles of Tc-stp-1 and TcM-1309 in the testis and vas deferens, suggesting important involvement of these genes in spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Zhou

Song

et al. 2015

Acta Tropica

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Zhou

Song

et al. 2015

Acta Tropica

View full text Add to dashboard Cite

“…Parasite material and slide preparation, RNA probe synthesis, and ISH procedures were performed as described previously (34). For these experiments, 10-μm frozen sections cut from adult B. malayi worms were used.…”

Section: Methodsmentioning

confidence: 99%

Interdomain lateral gene transfer of an essential ferrochelatase gene in human parasitic nematodes

Novelli

Jiang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Lateral gene transfer events between bacteria and animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral gene transfer in animal parasitic nematodes. Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; however, the human filarial parasite Brugia malayi has acquired a bacterial gene encoding ferrochelatase (BmFeCH), the terminal step in heme biosynthesis. BmFeCH, encoded by a 9-exon gene, is a mitochondrial-targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies. Homologs have been identified in several filariae and a nonfilarial nematode. RNAi and ex vivo inhibitor experiments indicate that BmFeCH is essential for viability, validating it as a potential target for filariasis control.

show abstract

“…Recent thought is that validation of proven microarray platforms is unnecessary because the strict statistical analysis of the primary microarray data is validation in and of itself (Yang and Speed 2002;Morey et al 2006). In our case, the B. malayi microarray platform has been extensively validated through a combination of microarray, quantitative reverse transcriptase PCR (qPCR), and tissue localization by in situ hybridization studies Li et al2004;Jiang et al 2008), therefore we did not report further validation of the results in the submitted manuscript (Chapter I). We did, as a matter of course, verify the differential expression of two candidates from the B. malayi dataset by qPCR, and provide these data as well as correlations of candidate transcripts to B. malayi genes' these data are the subject of Chapter II.…”

Section: ) Microarray Results Have Historically Been Verified By Othmentioning

confidence: 99%

“…and tissue localization by in situ hybridization of a panel of gender-associated transcripts (Jiang et al 2008).…”

Section: Mosquito Infectivity Assaysmentioning

confidence: 99%

Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity

et al. 2009

View full text Add to dashboard Cite

Filarial nematodes are a family of insect-borne parasitic worms that cause a number of diseases in man and animals. The most widespread filarial disease of humans is lymphatic filariasis, caused by worms in the genera Wuchereria and Brugia. Lymphatic filariasis is an economic and social burden in endemic countries and affects approximately 119 million people worldwide. Brugia malayi and B. pahangi microfilariae (mf) require a maturation period of at least five days in the mammalian host before they can infect mosquito vectors. This maturation process correlates with changes in the surface composition of mf that likely are associated with changes in gene expression. To test this hypothesis, we verified the differential infectivity of immature (<3 day) and mature (>30 day) Brugia mf for black-eyed Liverpool strain of Aedes aegypti (LVP), and then assessed transcriptome changes associated with microfilarial maturation by competitively hybridizing microfilarial cDNAs to the B. malayi oligonucleotide microarray. We identified transcripts that were more abundant in immature (94 in B. pahangi and 29 in B. malayi) and mature (64 in B. pahangi and 14 in B. malayi) mf. In each case, >40% of Brugia transcripts shared no similarity to known genes, or were similar to genes with unknown function; the remaining transcripts were categorized by putative function based on sequence similarity to known genes/proteins. Microfilarial maturation was not associated with demonstrable changes in the abundance of transmembrane or secreted proteins; however, differences in transcript abundance were observed for many that have predicted functions. For example, immature mf were enriched for transcripts putatively associated with immune modulation, neurotransmission, transcription and cellular cytoskeleton elements. In mature mf, there was an increase in transcripts potentially encoding hypodermal/muscle and surface molecules, i.e., cuticular collagens and sheath components. These finding lend support to the underlying hypothesis that changes in microfilarial gene expression drive surface modifications that influence the parasite to begin development in compatible vectors. Brugia malayi genes corresponding to differentially abundant transcripts were identified, and transcript abundance validated by quantitative polymerase chain reaction. These studies serve as a starting point towards gaining a better understanding of the parasite side of the intricate parasite/mosquito relationship.

show abstract

Localization of gender-regulated gene expression in the filarial nematode Brugia malayi

Cited by 25 publications

References 25 publications

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Molecular mechanism of serine/threonine protein phosphatase 1 (PP1cα–PP1r7) in spermatogenesis of Toxocara canis

Interdomain lateral gene transfer of an essential ferrochelatase gene in human parasitic nematodes

Use of microarray hybridization to identify Brugia genes involved in mosquito infectivity

Contact Info

Product

Resources

About