Localization of ghrelin in the epithelial cells of the duodenum in the rat

Abstract

Ghrelin is a hormone discovered relatively recently, the effect of which is associated with the normal activity of the digestive tract. It is the most abundant in the gastric mucosa, but also occurs in other parts of the digestive system, sexual organs and some glands. Many authors associate the action of ghrelin with appetite and weight gain or weight loss. The purpose of this study was by immunohistochemical methods to localize grelin-pozitive cells in the duodenal mucosa and comparatively to determine their amount. We use Ghrelin (H-40) antibody (Santa Cruz-USA), and for the visualization of the results-detection system EnVisionTM FLEX Mini Kit, High Ph (DAKO-k8024 HRP). Histomorphometric study performed by Michael Vinther Image Analyzer, Version 1.33 (Mee Soft, Germany).