Localization of H-2 antigens on mouse trophoblast cells.

Chatterjee‐Hasrouni, Saswati; Lala, Peeyush K.

doi:10.1084/jem.149.5.1238

Cited by 79 publications

(12 citation statements)

References 29 publications

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“…The physiology of placentation and immunological recognition by cytotoxic cells have both been subject to intense evolutionary selection, and there are marked anatomical and immunological differences between murine and human pregnancy (23). Furthermore, the variability of the murine data and supraphysiological magnitude of fetal-specific T cells that often develop in transgenic models mean that insights into the natural physiology of human alloreactive immunity require studies in pregnant women (24).…”

mentioning

confidence: 99%

Fetal-Specific CD8+ Cytotoxic T Cell Responses Develop during Normal Human Pregnancy and Exhibit Broad Functional Capacity

Lissauer

Piper

Goodyear

et al. 2012

The Journal of Immunology

120

115

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Tolerance of the semiallogeneic fetus presents a significant challenge to the maternal immune system during human pregnancy. T cells with specificity for fetal epitopes have been detected in women with a history of previous pregnancy, but it has been thought that such fetal-specific cells were generally deleted during pregnancy as a mechanism to maintain maternal tolerance of the fetus. We used MHC-peptide dextramer multimers containing an immunodominant peptide derived from HY to identify fetal-specific T cells in women who were pregnant with a male fetus. Fetal-specific CD8+ T lymphocytes were observed in half of all pregnancies and often became detectable from the first trimester. The fetal-specific immune response increased during pregnancy and persisted in the postnatal period. Fetal-specific cells demonstrated an effector memory phenotype and were broadly functional. They retained their ability to proliferate, secrete IFN-γ, and lyse target cells following recognition of naturally processed peptide on male cells. These data show that the development of a fetal-specific adaptive cellular immune response is a normal consequence of human pregnancy and that unlike reports from some murine models, fetal-specific T cells are not deleted during human pregnancy. This has broad implications for study of the natural physiology of pregnancy and for the understanding of pregnancy-related complications.

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mentioning

confidence: 99%

Fetal-Specific CD8+ Cytotoxic T Cell Responses Develop during Normal Human Pregnancy and Exhibit Broad Functional Capacity

Lissauer

Piper

Goodyear

et al. 2012

The Journal of Immunology

120

115

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“…Preimplantation mouse blastocysts implant in the endometrium and grow successfully despite their expression of H-2 antigens (27). Similarly, studies from our laboratory have unequivocably demonstrated the presence of class I major histocompatibility complex (MHC) (H-2K and D) antigens on mouse trophoblast cells both in single cell preparations (19) as well as in situ on their sinusoldal face exposed to the maternal blood, 2 yet they do not evoke an immunological rejection by the mother. Whether the poor immunogenicity of trophoblasts owes to the paucity of class II MHC (Ia) antigens on their surface (28), or is a result of some local immunosuppressor mechanism, remains undetermined.…”

Section: Discussionmentioning

confidence: 78%

“…Spleens were minced on a wire screen (80 mesh/in 2) with iris scissors in ice-cold 10% NCS-MEM, subjected to a clump removal procedure by layering on NCS, erythrocyte lysis by treatment with 0.168 M NH4CI (19), and resuspended at Abbreviations used in this paper: NCS, newborn calf serum; NMS, normal mouse serum; MEM, minimum essential medium; MHC, major histocompatibility complex. a concentration of 20 X 106 cells/ml of the same medium.…”

Section: Evaluation Of Chimerismmentioning

confidence: 99%

Bone marrow origin of decidual cell precursors in the pseudopregnant mouse uterus.

Kearns¹,

Lala²

1982

The Journal of Experimental Medicine

Self Cite

103

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Decidual cells are considered to be the endproduct of a hormonally induced transformation of endometrial stromal cells of the uterus. However, the source of these precursors remains unknown. This study of evaluated the possibility of their bone marrow origin by an examination of the H-2 phenotype of decidual cells in pseudopregnant bone marrow chimeras. These chimeras were produced by repopulating lethally irradiated CBA/J female (H-2k) mice with bone marrow from (CBA/J x C57BL/6J) F1 female (H-2kb) mice. Pseudopregnancy was produced with a hormonal regimen followed by an oil-induced decidual stimulus. Chimerism was evaluated radioautographically by an identification of the donor-specific Kb phenotype on cells with an immunolabeling technique with monospecific anti-H-2 serum followed by radioiodinated protein A. The extent of chimerism as indicated by the degree of Kb labeling on decidual cells as well as macrophages contained within the decidual nodules was quantitatively compared with that seen on splenic lymphocytes. Fair to good chimerism, as reflected by labeling for the donor-specific marker (Kb), was seen on splenic lymphocytes and macrophages within the decidual nodules in 6 out of 11 animals. A similar level of chimerism was detected on decidual cells in all but one of these six, in which case this was low. One animal showed low chimerism in the spleen but good chimerism on the decidual cells. The remaining four mice were nonchimeric for all three cell types. These results indicate that decidual cells and macrophages appearing within the decidual nodules of pseudopregnant mice are ultimate descendants of bone marrow cells.

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“…Cells were subjected to either a three-step sandwich labeling by a sequential exposure to goat antimouse IgG (to mask natively bound IgG) followed by monospecific antibod of either parental haplotype and then jee-Hasrouni and Lala, 1979), or a two-step sandwich labeling with monospecific antibodies followed by 1251-labeled protein A (Chatterjee-Hasrouni and Lala, 19811, or a direct labeling with 1251-labeled monoclonal antibodies (V. Colavincenzo and P.K. Lala, unpublished).…”

Section: The Question Of Antigenicity1 Immunogenicity Of the Fetal Mementioning

confidence: 99%

Cells of the fetomaternal interface: Their role in the maintenance of viviparous pregnancy

1984

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An immune system capable of discriminating between self and nonself evolved in nature long before the appearance of the viviparous mode of pregnancy, which brings maternal cells into a direct physical contact with genetically disparate cells of fetal origin. In the hemochorial type of placentation, the former include cells of the maternal immune system. This article briefly reviews the possible mechanisms that may protect the semiallogeneic conceptus in nature, with special reference to the role of the cells at the fetomaternal interface. We also present some new data on the antigenicity of pre- and postimplantation trophoblast cells and the immunobiology of decidual cells. Systemic changes in the maternal immune system appear to represent homeostatic responses to the presence of a semiallogeneic conceptus, unrelated to its protection; mechanisms for this protection must reside locally at the fetomaternal interface. We find that the lack of immunogenicity of the outer (trophoblast) cells of the preimplantation blastocyst can be explained by a transient disappearance of the major histocompatibility (MHC) antigens on their cell surface. However, following implantation and the formation of the placenta, class 1 MHC antigens reappear on certain classes of trophoblast cells, i.e., labyrinthine and spongiotrophoblast cells of the murine placenta. Similarly, cytotrophoblast cells of the early human placenta exhibit the presence of class 1 MHC antigens. An absence of class 2 MHC antigens despite the presence of class 1 antigens cannot entirely explain the lack of trophoblast immunogenicity. A local immunosuppression mediated by trophoblast cells themselves as well as maternal cells of hemopoietic origin in the decidua remain as a strong possibility. Typical decidual cells appear to play a central role in the maintenance of pregnancy because of their numerous functions: nutritive, endocrine, and immunoregulatory. Our studies reveal that they are descendants of bone-marrow-derived precursors, have unique surface markers recognizable with monoclonal antibodies nonreactive with other hemopoietic cell lineages, and have the ability to abrogate mixed lymphocyte reactions in vitro in a genetically unrestricted manner. Further studies directed at the cells of the fetomaternal interface should provide a better insight into the mode of survival of the nature's most commonplace allograft.

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Localization of H-2 antigens on mouse trophoblast cells.

Cited by 79 publications

References 29 publications

Fetal-Specific CD8+ Cytotoxic T Cell Responses Develop during Normal Human Pregnancy and Exhibit Broad Functional Capacity

Fetal-Specific CD8+ Cytotoxic T Cell Responses Develop during Normal Human Pregnancy and Exhibit Broad Functional Capacity

Bone marrow origin of decidual cell precursors in the pseudopregnant mouse uterus.

Cells of the fetomaternal interface: Their role in the maintenance of viviparous pregnancy

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