Localization of Hg and Pb in the palps, the digestive gland and the gills in Mytilus galloprovincialis (L.) using autometallography and X-ray microanalysis

Dimitriadis, V.K.; Domouhtsidou, G.P.; Raftopoulou, E.K.

doi:10.1016/s0269-7491(03)00122-2

Cited by 30 publications

(13 citation statements)

References 47 publications

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“…The observed localization of aroclor 1254 corresponds with several reported effects of aroclor 1254 on signal transduction systems such as calcium homeostasis, phosphoinositol hydrolysis, and protein kinase C in mitochondria (Tilson et al, 1998). In other studies, various cellular and subcellular pathological alterations in Mytilidae have been observed after laboratory exposure to Pb, PCBs and PAHs (Sunila, 1988;Lowe and Clarke, 1989;Dimitriadis et al, 2003). In the present study, cytopathological alterations after Pb, PCBs and PAHs exposure in cell organelles and compartments suggest a close causeeffect relationship to the subcellular localization of the contaminants.…”

supporting

confidence: 77%

Immuno-localisations (GSSP) of subcellular accumulation sites of phenanthrene, aroclor 1254 and lead (Pb) in relation to cytopathologies in the gills and digestive gland of the mussel Mytilus edulis

Einsporn

Köehler

2008

Marine Environmental Research

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Cell and tissue pathology of both, gill and digestive tissue, has been the subject of many studies for the elucidation of contaminant-induced biological effects. In the present study, cellular pathological alterations were linked to subcellular sites of chemical accumulation in gills and digestive gland tissues. For this purpose, mussels (Mytilus edulis) were exposed to the organic contaminants aroclor 1254 (PCB) (20µg/L), phenanthrene (PAH) (150µg/L) or the metal lead (Pb) (2.5mg/L). The localization of chemicals at the subcellular level was analysed by an antibody-based detection system (GSSP) by the use of commercially available antibodies specifically directed against the chemicals. Pathological changes were analysed in parallel in identical samples by transmission electron microscopy. After exposure to the different contaminants, cell organelles such as mitochondria, the endo-lysosomal system as well as endoplasmic reticulum showed clear evidence of chemically-induced

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supporting

confidence: 77%

Immuno-localisations (GSSP) of subcellular accumulation sites of phenanthrene, aroclor 1254 and lead (Pb) in relation to cytopathologies in the gills and digestive gland of the mussel Mytilus edulis

Einsporn

Köehler

2008

Marine Environmental Research

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“…Actually, the removal of the metal ions from cytosol by increased MT synthesis, compartmentalization into the endo-lysosomal system and mineralized granules can enhance the uptake and accumulation processes. Autometallography, a technique detecting metal ions by transforming them into insoluble sulfides, have confirmed the localization of Cd, Hg, Ag, Pb, Cu and Zn in either digestive cells, gill cells or haemocytes of mussels [22], [26], [27].…”

Section: Introductionmentioning

confidence: 88%

“…Depending on the element and its intracellular concentration, metals can be released also from haemocytes, digestive cells, nephrocytes, and eventually eliminated with faeces and urine [22], [26]. Following mussel exposure to 100 µg/L Hg for 30–60 days, metal accumulation and release from the apical and basal regions of epithelial gill cells have been reported, and a decrease in metal accumulation due for instance to adaptive cell renewal during long-term exposure has been hypothesized [27].…”

Section: Introductionmentioning

confidence: 99%

DNA Damage and Transcriptional Changes in the Gills of Mytilus galloprovincialis Exposed to Nanomolar Doses of Combined Metal Salts (Cd, Cu, Hg)

et al. 2013

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Aiming at an integrated and mechanistic view of the early biological effects of selected metals in the marine sentinel organism Mytilus galloprovincialis, we exposed mussels for 48 hours to 50, 100 and 200 nM solutions of equimolar Cd, Cu and Hg salts and measured cytological and molecular biomarkers in parallel. Focusing on the mussel gills, first target of toxic water contaminants and actively proliferating tissue, we detected significant dose-related increases of cells with micronuclei and other nuclear abnormalities in the treated mussels, with differences in the bioconcentration of the three metals determined in the mussel flesh by atomic absorption spectrometry. Gene expression profiles, determined in the same individual gills in parallel, revealed some transcriptional changes at the 50 nM dose, and substantial increases of differentially expressed genes at the 100 and 200 nM doses, with roughly similar amounts of up- and down-regulated genes. The functional annotation of gill transcripts with consistent expression trends and significantly altered at least in one dose point disclosed the complexity of the induced cell response. The most evident transcriptional changes concerned protein synthesis and turnover, ion homeostasis, cell cycle regulation and apoptosis, and intracellular trafficking (transcript sequences denoting heat shock proteins, metal binding thioneins, sequestosome 1 and proteasome subunits, and GADD45 exemplify up-regulated genes while transcript sequences denoting actin, tubulins and the apoptosis inhibitor 1 exemplify down-regulated genes). Overall, nanomolar doses of co-occurring free metal ions have induced significant structural and functional changes in the mussel gills: the intensity of response to the stimulus measured in laboratory supports the additional validation of molecular markers of metal exposure to be used in Mussel Watch programs.

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“…0.1 mg/L의 Hg에 노출된 지중해담치의 소화선세관 소화세포내에는 지방갈색소가 증가하였다 (Dimitriadis et al, 2003). 대복을 24주 동안 0.64, 1.07, 1.79 mg/L의 Zn에 노출 시킨 결과, 지방갈색소는 농도의존적으로 증가하였다 (Ju et al, 2006).…”

Section: 중금속 가운데unclassified

Concentration of Trace Metals, Intersexuality and Histological Alterations of Ruditapes philippinarum from Gamak Bay in Korea

Jeon¹,

Kim²,

Choi

et al. 2015

The Korean Journal of Malacology

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This study investigated concentration of trace metals, intersexuality and histopathology of digestive gland in Ruditapes philippinarum. Samples were collected from three areas of Gamak Bay in May, 2010. The concentration of trace metals showed that Al was highest, Zn was second high concentration and the lowest concentration was Cd. The intersexuality was 18.5% and female was higher than the male. Intersex type was observed one type. Destruction of digestive tubule epithelium was highest among other biomarkers in digestive gland. Distribution of basophilic cell was 5.8% and lipofuscin was 1.8%.

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Localization of Hg and Pb in the palps, the digestive gland and the gills in Mytilus galloprovincialis (L.) using autometallography and X-ray microanalysis

Cited by 30 publications

References 47 publications

Immuno-localisations (GSSP) of subcellular accumulation sites of phenanthrene, aroclor 1254 and lead (Pb) in relation to cytopathologies in the gills and digestive gland of the mussel Mytilus edulis

Immuno-localisations (GSSP) of subcellular accumulation sites of phenanthrene, aroclor 1254 and lead (Pb) in relation to cytopathologies in the gills and digestive gland of the mussel Mytilus edulis

DNA Damage and Transcriptional Changes in the Gills of Mytilus galloprovincialis Exposed to Nanomolar Doses of Combined Metal Salts (Cd, Cu, Hg)

Concentration of Trace Metals, Intersexuality and Histological Alterations of Ruditapes philippinarum from Gamak Bay in Korea

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