Localization of IgE Synthesis in Immediate-Type Allergy of the Upper Respiratory Tract (With 3 color plates)

Ganzer, U.; Bachert, Claus

doi:10.1159/000276000

Cited by 45 publications

(30 citation statements)

References 21 publications

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“…Clinical studies have found a close association between asthma and serum IgE levels, and the amount of allergen-specific IgE can be dramatically increased in sensitized or atopy patients (7,8). Allergen-specific IgE is located in the human airway, such as in nasal mucosa from allergic patients who suffer allergic rhinitis in sinonasal tissue, nasal polyposis, and allergic fungal rhinosinusitis (9)(10)(11)(12). This observation is further supported by the fact that B cells from the nasal mucosa of allergic individuals locally synthesize IgE (13,14).…”

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confidence: 99%

CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells

Palaniyandi

Tomei

et al. 2011

The Journal of Immunology

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IgE-mediated allergic inflammation occurs when allergens cross-link IgE on the surface of immune cells, thereby triggering the release of inflammatory mediators as well as enhancing Ag presentations. IgE is frequently present in airway secretions, and its level can be enhanced in human patients with allergic rhinitis and bronchial asthma. However, it remains completely unknown how IgE appears in the airway secretions. In this study, we show that CD23 (FcεRII) is constitutively expressed in established or primary human airway epithelial cells, and its expression is significantly upregulated when airway epithelial cells were subjected to IL-4 stimulation. In a transcytosis assay, human IgE or IgE-derived immune complex (IC) was transported across a polarized Calu-3 monolayer. Exposure of the Calu-3 monolayer to IL-4 stimulation also enhanced the transcytosis of either human IgE or the IC. A CD23-specific Ab or soluble CD23 significantly reduced the efficiency of IgE or IC transcytosis, suggesting a specific receptor-mediated transport by CD23. Transcytosis of both IgE and the IC was further verified in primary human airway epithelial cell monolayers. Furthermore, the transcytosed Ag–IgE complexes were competent in inducing degranulation of the cultured human mast cells. Because airway epithelial cells are the first cell layer to come into contact with inhaled allergens, our study implies CD23-mediated IgE transcytosis in human airway epithelial cells may play a critical role in initiating and contributing to the perpetuation of airway allergic inflammation.

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confidence: 99%

CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells

Palaniyandi

Tomei

et al. 2011

The Journal of Immunology

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“…The nasal mucosa itself contains all the cell types (dendritic cells, T-cells and B-cells [8] ) and cytokines (interleukin (IL)-4 and IL-13 [9±12]) necessary for an IgE immunoresponse. Other studies have suggested that IgE synthesis takes place in the downstream cervical lymph nodes [13,14] or upper respiratory and lower respiratory lymph nodes [15]. However no information has been obtained as to whether the IgE comes from the circulation (transudate) or from the mucosal glands (exudatate).…”

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confidence: 99%

Local production and detection of (specific) IgE in nasal B-cells and plasma cells of allergic rhinitis patients

et al. 2000

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Allergic diseases are characterized by allergic complaints in the shock organ and specific immunoglobulin (Ig)E in serum. Literature data indicate that the nasal mucosa itself could produce at least a large part of the specific IgE in allergic rhinitis patients.In order to investigate this hypothesis, nasal mucosal biopsies from the inferior turbinate were taken from symptomatic grass pollen allergic rhinitis patients, symptomatic house dust mite allergic rhinitis patients and nonallergic healthy controls, confirmed by radioallergosorbent test and skin-prick test. Immunohistochemical double-staining was performed for B-cells (CD19) with IgE, plasma cells (CD138) with IgE and plasma cells with biotinylated allergens.Significantly more IgE-positive B-cells and IgE-positive plasma cells were found in the nasal mucosa of allergic patients than in that of nonallergic controls. Double staining with biotinylated allergens and plasma cells showed allergen-positive plasma cells in the nasal mucosa of allergic patients and no allergen-positive plasma cells in the nasal mucosa of nonallergic patients. Blocking experiments using polyclonal antibodies directed against IgE showed a significant reduction in the number of allergenpositive cells in contrast to experiments using polyclonal antibodies directed against IgG, IgA or IgM.This study describes new evidence that specific immunoglobulin E is produced locally in the nasal mucosa in patients with seasonal allergic rhinitis and perennial allergic rhinitis, but not in nonallergic controls. Eur Respir J 2000; 15: 491±497.

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“…Ganzer and Bachert (12) demonstrated that the production of IgE is not only limited to the nasal mucosa but also exists in the lymphatic tissue in Waldeyer's ring.…”

Section: Discussionmentioning

confidence: 99%

Adenoid Vegetation in Children with Allergic Rhinitis

Bozkurt¹,

Dizdar²,

Korkut³

et al. 2016

Turk Arch Otorhinolaryngol

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Objective: Pediatric patients with nasal obstruction due to adenoid vegetation (AV) can also encounter allergic rhinitis (AR) as a comorbidity. The aim of the study was to estimate the incidence of mite sensitization and its effect on adenoid size in children who underwent adenoidectomy.Methods: This prospective randomized study conducted between August and September 2014 included 84 children. Skin Prick Test (SPT) for inhalant allergens was preoperatively applied to all children who underwent adenoidectomy for nasal obstruction. Children were divided into two study groups: AV only (Group I) (n=52) and AV with Dermatophagoides Pteronyssinus and/or D. farinae allergy (Group II) (n=32). Postoperative specimen volumes, visual analogue scale (VAS) scores, and adenoid volumes measured using flexible fiberoptic nasopharyngolaryngoscopy were compared between the two groups.Results: Postoperative specimen volume measures were higher in Group II compared with those in Group I (p<0.05). Furthermore, in preoperative endoscopic examination, adenoid volume measures were higher in Group II compared with those in Group I (p<0.05). Pre and postoperative VAS scores in SPT+ group were higher in the Group II (p<0.05) than those in Group I. Conclusion:We observed that children with AR tend to have an early onset of symptoms of adenoid hypertrophy. We believe that focusing on the management of role of allergy regarding these early symptoms will reduce the need for surgery in a large number of cases. We suggest that SPT must be performed in all children with AV and adenoid examination should not be neglected in children with AR. Keywords: Adenoid vegetation, allergic rhinitis, adenoid volume

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Localization of IgE Synthesis in Immediate-Type Allergy of the Upper Respiratory Tract (With 3 color plates)

Cited by 45 publications

References 21 publications

CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells

CD23-Dependent Transcytosis of IgE and Immune Complex across the Polarized Human Respiratory Epithelial Cells

Local production and detection of (specific) IgE in nasal B-cells and plasma cells of allergic rhinitis patients

Adenoid Vegetation in Children with Allergic Rhinitis

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