Localization of m2 muscarinic receptor protein in parvalbumin and calretinin containing cells of the adult rat entorhinal cortex using two complementary methods

Chaudhuri, Joydeep D.; Hiltunen, Mikko; Nykänen, M.; Ylä‐Herttuala, Seppo; Miettinen, Reijo

doi:10.1016/j.neuroscience.2004.11.032

Cited by 13 publications

(17 citation statements)

References 27 publications

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“…In the entorhinal cortex 88% of PV+ neurons and 26% of CR+ neurons were M2R+, but other markers were not investigated (Chaudhuri et al, 2005). In the hippocampus virtually all M2R+ neurons are GABAergic, but only about 50% exhibit immunoreactivity for the interneuronal markers PV, CB, SOM, VIP, CR, or CCK (Hajos et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Neuronal localization of M2 muscarinic receptor immunoreactivity in the rat amygdala

McDonald

Mascagni

2011

Neuroscience

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Muscarinic cholinergic neurotransmission in the amygdala is critical for memory consolidation in emotional/motivational learning tasks, but little is known about the neuronal distribution of different receptor subtypes. Immunohistochemistry was used in the present investigation to localize the m2 receptor (M2R). Differential patterns of M2R-immunoreactivity (m2R-ir) were observed in the somata and neuropil of the various amygdalar nuclei. Neuropilar m2R-ir was strongest in rostral portions of the basolateral nuclear complex (BLC). M2R+ somata were seen in low numbers in all nuclei of the amygdala. Most M2R+ neurons associated with the BLC were in the lateral nucleus and external capsule. These cells were nonpyramidal neurons that contained glutamatic acid decarboxylase (GAD), somatostatin (SOM) and neuropeptide Y (NPY), but not parvalbumin (PV), calretinin (CR), or cholecystokinin (CCK). Little or no M2R-ir was observed in GAD+, PV+, CR+, or CCK+ axons in the BLC, but was seen in some SOM+ axons and many NPY+ axons. M2R-ir was found in a small number of spiny and aspiny neurons of the central nucleus that were mainly located along the lateral and ventral borders of its lateral subdivision. Many of these cells contained SOM and NPY. M2R+ neurons were also seen in the medial nucleus, including a distinct subpopulation of neurons that surrounded its anteroventral subdivision. The latter neurons were negative for all neuronal markers analyzed. The intercalated nuclei (IN) were associated with two types of large M2R+ neurons, spiny and aspiny. The small principal neurons of the INs were M2R-negative. The somata and dendrites of the large spiny neurons, which were actually found in a zone located just outside of the rostral INs, expressed SOM and NPY, but not GAD. These findings indicate that acetylcholine can modulate a variety of discrete neuronal subpopulations in various amygdalar nuclei via M2Rs, especially neurons that express SOM and NPY.

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Section: Discussionmentioning

confidence: 99%

Neuronal localization of M2 muscarinic receptor immunoreactivity in the rat amygdala

McDonald

Mascagni

2011

Neuroscience

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“…In the rat, morphologically comparable CR+ multipolar neurons, albeit with smaller somata exist. The aspiny dendrites of these neurons extend horizontally for a short distance, eventually turning towards the pia or extending into layer II, occasionally also reaching layer III (Figure c; Chaudhuri et al., ; Miettinen, Pitkänen, & Miettinen, ; Wouterlood, van Denderen, van Haeften, & Witter, ).…”

Section: Layer Imentioning

confidence: 96%

Neuronal chemo‐architecture of the entorhinal cortex: A comparative review

Kobro‐Flatmoen

2019

Eur J of Neuroscience

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The identification of neuronal markers, that is, molecules selectively present in subsets of neurons, contributes to our understanding of brain areas and the networks within them. Specifically, recognizing the distribution of different neuronal markers facilitates the identification of borders between functionally distinct brain areas. Detailed knowledge about the localization and physiological significance of neuronal markers may also provide clues to generate new hypotheses concerning aspects of normal and abnormal brain functioning. Here, we provide a comprehensive review on the distribution within the entorhinal cortex of neuronal markers and the morphology of the neurons they reveal. Emphasis is on the comparative distribution of several markers, with a focus on, but not restricted to rodent, monkey and human data, allowing to infer connectional features, across species, associated with these markers, based on what is revealed by mainly rodent data. The overall conclusion from this review is that there is an emerging pattern in the distribution of neuronal markers in the entorhinal cortex when aligning data along a comparable coordinate system in various species.

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“…Alike SOM+ interneurons, VIP+ bipolar neurons are depolarized by muscarinic agonists (Kawaguchi, 1997; Fanselow et al, 2008). Expression of m2 receptors has been reported in a minority CR+ interneurons of the rat entorhinal cortex (Chaudhuri et al, 2005) but the subtype-specific identity of the muscarinic receptors inducing the depolarization of VIP+ bipolar interneurons remains unknown. This indicates that CR+ interneurons integrate cholinergic afferences from the basal forebrain (Figure 5) as recently shown for ChAT+ bipolar interneurons using optogenetics (Arroyo et al, 2012), which might be important for behavioral state-dependent control of cortical circuits (Pi et al, 2013).…”

Section: Synaptic Inputsmentioning

confidence: 99%

Revisiting enigmatic cortical calretinin-expressing interneurons

et al. 2014

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Cortical calretinin (CR)-expressing interneurons represent a heterogeneous subpopulation of about 10–30% of GABAergic interneurons, which altogether total ca. 12–20% of all cortical neurons. In the rodent neocortex, CR cells display different somatodendritic morphologies ranging from bipolar to multipolar but the bipolar cells and their variations dominate. They are also diverse at the molecular level as they were shown to express numerous neuropeptides in different combinations including vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK), neurokinin B (NKB) corticotrophin releasing factor (CRF), enkephalin (Enk) but also neuropeptide Y (NPY) and somatostatin (SOM) to a lesser extent. CR-expressing interneurons exhibit different firing behaviors such as adapting, bursting or irregular. They mainly originate from the caudal ganglionic eminence (CGE) but a subpopulation also derives from the dorsal part of the medial ganglionic eminence (MGE). Cortical GABAergic CR-expressing interneurons can be divided in two main populations: VIP-bipolar interneurons deriving from the CGE and SOM-Martinotti-like interneurons originating in the dorsal MGE. Although bipolar cells account for the majority of CR-expressing interneurons, the roles they play in cortical neuronal circuits and in the more general metabolic physiology of the brain remained elusive and enigmatic. The aim of this review is, firstly, to provide a comprehensive view of the morphological, molecular and electrophysiological features defining this cell type. We will, secondly, also summarize what is known about their place in the cortical circuit, their modulation by subcortical afferents and the functional roles they might play in neuronal processing and energy metabolism.

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Localization of m2 muscarinic receptor protein in parvalbumin and calretinin containing cells of the adult rat entorhinal cortex using two complementary methods

Cited by 13 publications

References 27 publications

Neuronal localization of M2 muscarinic receptor immunoreactivity in the rat amygdala

Neuronal localization of M2 muscarinic receptor immunoreactivity in the rat amygdala

Neuronal chemo‐architecture of the entorhinal cortex: A comparative review

Revisiting enigmatic cortical calretinin-expressing interneurons

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