Localization of nucleoside diphosphatase in the onion root tip

Goff, C. W.

doi:10.1007/bf01275775

Cited by 45 publications

(21 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The second peak of IDPase activity may be associated with the tonoplast. Histochemical studies show that IDPase activity is associated with a number of cellular membrane systems including endoplasmic reticulum, Golgi, plasma membrane and tonoplast (7,27 4 and 6). Likewise, when the 13,000 to 80,000g fraction was centrifuged in the sucrose gradient for 1.5 hr, pH 6.5-ATPase activity was broadly distributed in the gradient with a peak at a lower density than that observed when the gradient was centrifuged for 15 hr (compare Figs.…”

mentioning

confidence: 99%

Isolation of Plasma Membranes from Corn Roots by Sucrose Density Gradient Centrifugation

Leonard¹,

VanDerWoude²

1976

Plant Physiol.

View full text Add to dashboard Cite

An investigation was conducted into the isolation of plasma membrane vesicles from primary roots of corn (Zea mays L., WF9 x M14) by sucrose density gradient centrifugation. Identification of plasma membranes in cell fractions was by specific staining with the periodic-chromic-phosphotungstic acid procedure. Plasma membrane vescles were rich in K+-stimulated ATPase activity at pH 6.5, and equilibrated in linear gradients of sucrose at a peak density of about 1.165 g/cc. It was necessary to remove mitochondria (equilibrium density of 1.18 g/cc) from the homogenate before density gradient centrifugation to minimize mitochondrial contamination of the plasma membrane fraction. Endoplasmic reticulum (NADH-cytochrome c reductase) and Golgi apparatus (latent IDPase) had equilibrium densities in sucrose of about 1.10 g/cc and 1.12 to 1.15 g/cc, respectively. A correlation (r = 0.975) was observed between K+-stimulated ATPase activity at pH 6.5 and the content of plasma membranes in various cell fractions. ATPase activity at pH 9 and cytochrome c oxidase activity were also correlated.A major peak of ATPase activity at pH 6.5 was observed at low density in Ficoll after nonequilibrium centrifugation in a combination Ficollsucrose gradient. Twenty to forty percent of the vesicles in this ATPase fraction stained positively for plasma membranes, and with equilibrium centrifugation the major portion of the ATPase activity shifted to densities in sucrose which were characteristic of plasma membranes. All major vesicular ATPase activities observed in Ficoll or sucrose contained substantial amounts of plasma membranes. For unknown reasons, mitochondria and plasma membranes equilibrated over a broader density range and at lower peak densities in sucrose as a result of equilibrium centrifugation through Ficoll.

show abstract

mentioning

confidence: 99%

Isolation of Plasma Membranes from Corn Roots by Sucrose Density Gradient Centrifugation

Leonard¹,

VanDerWoude²

1976

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…According to Goff (1973), neutral phosphatase activity is localized at sites of active glycosylation, so that the neutral phosphatase activity localized on the newly-synthesized host membrane around P. nodositatum and in the endoplasmic reticulum network of infected cells further suggests that polysaccharide synthesis occurs at the plant-fungal interface. A similar conclusion was drawn for endomycorrhiza by Jeanmaire et al (1985) who showed that a neutral phosphatase activity occurs on the plant membrane around intracellular fungal arbuscules.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of cortical root cells of Alnus incana infected by Penicillium nodositatum

et al. 1995

View full text Add to dashboard Cite

SUMMARYPenicillium nodositatum Valla penetrates Alnus incana (L.) Moench roots through deformed root hairs. It then colonizes the cortical cells and induces the formation of myconodules which have structures similar to those of young actinorhizas. The ultrastructure of infected cortical cells has been compared with that of cells infected by pathogenic or symbiotic fungi as well as with that of cells infected by Frankia. Our observations show that an infected host cell shows few visible signs of resistance to the fungal invasion. As soon as P. nodositatum enters the cell, it is surrounded by a wall-like pecto-cellulosic matrix produced by the host cell. The host plasmalemma, invaginated around the endophyte, keeps its integrity as it does in symbiotic associations. At the beginning of the infection, the host cytoplasm and the fungus are intact but when the cell is completely invaded, the host cytoplasm becomes disorganized and subsequently the fungus also senesces, as in roots of Ericaceae infected by an endomycorrhizal fungus or as in older tissues of actinorhizas. P. nodositatum could survive the dead host cell by using its own reserves. Uninfected neighbouring host cells remain intact. P. nodositatum can be considered to behave as a neutral microsymbiont of A. incana, in a way similar to that of a compatible but ineffective Frankia strain.

show abstract

“…1961, Northcote et Pickett-Heaps 1966, Juniper et Roberts 1966. Dauwalder et al 1966, 1969, Harris et Northcote 1970, Rougier 1971, Goff 1973, Dexheimer et al 1978.…”

Section: Les Cellules De La Coiffe Des Racinesunclassified

“…11) sur les membranes des vesicules de secretion (Dauwalder et al 1969, Goff 1973, Dexheimer et al 1978. Pour Dauwalder et al (1969), cette activite enzymatique serait caracteristique des dictyosomes specialises dans la production de grander quantites de polysaccharides.…”

Section: Les Cellules De La Coiffe Des Racinesunclassified

Sur l'expression ultrastructurale de la differenciation des cellules vegetales.

Dexheimer¹

1983

CYTOLOGIA

View full text Add to dashboard Cite

Localization of nucleoside diphosphatase in the onion root tip

Cited by 45 publications

References 31 publications

Isolation of Plasma Membranes from Corn Roots by Sucrose Density Gradient Centrifugation

Isolation of Plasma Membranes from Corn Roots by Sucrose Density Gradient Centrifugation

Ultrastructure of cortical root cells of Alnus incana infected by Penicillium nodositatum

Sur l'expression ultrastructurale de la differenciation des cellules vegetales.

Contact Info

Product

Resources

About