Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats

Kim, Ji-Youn; Kim, Byung‐In; Jue, Seong-Suk; Park, Jae Hyun; Shin, Je-Won

doi:10.2319/030911-173.1

Cited by 31 publications

(25 citation statements)

References 24 publications

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“…The Osterix ( Osx )- Cre line has been used to selectively delete floxed alleles in committed osteoblasts and odontoblasts [30, 31], though the spatiotemporal expression of Osx in the wider periodontium has not been completely resolved [32-34]. While the Osx-Cre transgene has been reported to cause delayed or defective skeletal and craniofacial mineralization resulting from Osterix loss-of-function [35-37], studies including analysis of molar teeth have not identified similar dental defects [30, 38, 39].…”

Section: Resultsmentioning

confidence: 99%

Multiple essential MT1-MMP functions in tooth root formation, dentinogenesis, and tooth eruption

Snider

Wimer

et al. 2016

Matrix Biology

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Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a transmembrane zinc-endopeptidase that breaks down extracellular matrix components, including several collagens, during tissue development and physiological remodeling. MT1-MMP-deficient mice (MT1-MMP−/−) feature severe defects in connective tissues, such as impaired growth, osteopenia, fibrosis, and conspicuous loss of molar tooth eruption and root formation. In order to define the functions of MT1-MMP during root formation and tooth eruption, we analyzed the development of teeth and surrounding tissues in the absence of MT1-MMP. In situ hybridization showed that MT1-MMP was widely expressed in cells associated with teeth and surrounding connective tissues during development. Multiple defects in dentoalveolar tissues were associated with loss of MT1-MMP. Root formation was inhibited by defective structure and function of Hertwig's epithelial root sheath (HERS). However, no defect was found in creation of the eruption pathway, suggesting that tooth eruption was hampered by lack of alveolar bone modeling/remodeling coincident with reduced periodontal ligament (PDL) formation and integration with the alveolar bone. Additionally, we identified a significant defect in dentin formation and mineralization associated with the loss of MT1-MMP. To segregate these multiple defects and trace their cellular origin, conditional ablation of MT1-MMP was performed in epithelia and mesenchyme. Mice featuring selective loss of MT1-MMP activity in the epithelium were indistinguishable from wild type mice, and importantly, featured a normal HERS structure and molar eruption. In contrast, selective knock-out of MT1-MMP in Osterix-expressing mesenchymal cells, including osteoblasts and odontoblasts, recapitulated major defects from the global knock-out including altered HERS structure, short roots, defective dentin formation and mineralization, and reduced alveolar bone formation, although molars were able to erupt. These data indicate that MT1-MMP activity in the dental mesenchyme, and not in epithelial-derived HERS, is essential for proper tooth root formation and eruption. In summary, our studies point to an indispensable role for MT1-MMP-mediated matrix remodeling in tooth eruption through effects on bone formation, soft tissue remodeling and organization of the follicle/PDL region.

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Section: Resultsmentioning

confidence: 99%

Multiple essential MT1-MMP functions in tooth root formation, dentinogenesis, and tooth eruption

Snider

Wimer

et al. 2016

Matrix Biology

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“…Animal experiments have demonstrated that continuous tensile strain generated by orthodontic appliances also upregulates osteogenic markers in PDL during orthodontic tooth movement (Kawarizadeh et al, 2005; Kim et al, 2012; Watanabe et al, 2008). Therefore, our device induces similar biological effects in the cells.…”

Section: Discussionmentioning

confidence: 99%

Novel device for application of continuous mechanical tensile strain to mammalian cells

et al. 2017

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During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tensile strain to cells in culture. The continuous tensile strain was applied to human immortalized periodontal ligament cell line (HPL cells) and the cytoskeletal structures of HPL cells were examined by immunohistochemistry. The expression of both inflammatory and osteogenic markers was also examined by real-time reverse transcription polymerase chain reaction. The osteogenic protein, Osteopontin (OPN), was also detected by western blot analysis. The actin filaments of HPL cells showed uniform arrangement under continuous tensile strain. The continuous tensile strain increased the expression of inflammatory genes such as IL-1β, IL-6, COX-2 and TNF-α, and osteogenic genes such as RUNX2 and OPN in HPL cells. It also elevated the expression of OPN protein in HPL cells. These results suggest that our new simple device is useful for exploring the responses to continuous tensile strain applied to the cells.

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“…SPP1 is thought to promote or regulate the adhesion, attachment, and spreading of osteoclasts to the bone surface during bone resorption (Standal et al, 2004). Previous studies have demonstrated that mechanical stress-induced expression of SPP1 is mostly composed of, or is present in, almost all osteocytes and in some osteoblasts and bone-lining cells at the resorption site in the early stage of experimental tooth movement (Terai et al, 1999;Kim et al, 2012). Therefore, we chose SPP1 as the major indicators to observe the osteogenic differentiation of hPDLCs under CTS.…”

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confidence: 99%

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

Han

Sheng

et al. 2013

DNA and Cell Biology

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Physiological occlusal force constitutively exists in the oral environment and is important for periodontal homeostasis and remodeling. Cyclic tensile stress (CTS) triggers the biological response of periodontal ligament (PDL). However, a few reports have studied the correlation between CTS during physiological occlusal force and PDL cell activities such as osteogenic differentiation. In the present study, human PDL cells (hPDLCs) were subjected to 10% elongation CTS loading at 0.5 Hz for 24 h, which represents the physiological conditions of occlusal force. Gene expression microarray was used to investigate the mechano-induced differential gene profile and pathway analysis in vitro. The osteogenic relative factors, that is, SPP1, RUNX2, and SP7, were assessed by real-time PCR and Western blot. The involvement of mitogen-activated protein kinase (MAPK) signaling pathways was investigated by Western blot with a specific inhibitor. The expressions of SPP1, RUNX2, SP7, p-ERK1/2, and p-Elk1 were up-regulated after 10% CTS exposure. However, these up-regulated expressions were prevented by ERK1/2 inhibitor U0126 in the physiological occlusal force-applied hPDLCs. These results showed that 10% CTS could enhance osteogenic differentiation of hPDLCs via ERK1/2-Elk1 MAPK pathway, indicating that CTS during physiological occlusal force is a potent agent for PDL remodeling.

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Localization of osteopontin and osterix in periodontal tissue during orthodontic tooth movement in rats

Cited by 31 publications

References 24 publications

Multiple essential MT1-MMP functions in tooth root formation, dentinogenesis, and tooth eruption

Multiple essential MT1-MMP functions in tooth root formation, dentinogenesis, and tooth eruption

Novel device for application of continuous mechanical tensile strain to mammalian cells

Cyclic Tensile Stress During Physiological Occlusal Force Enhances Osteogenic Differentiation of Human Periodontal Ligament Cells via ERK1/2-Elk1 MAPK Pathway

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