Localization of pro-opiomelanocortin mRNA transcripts and peptide immunoreactivity in rat heart

Millington, William R.; Rosenthal, David W.; Ünal, Can; Nyquist-Battie, Cynthia

doi:10.1016/s0008-6363(99)00076-0

Cited by 39 publications

(18 citation statements)

References 56 publications

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“…It appears that there are multiple forms of POMC transcripts. Pituitary POMC mRNA encodes a secreted protein, whereas certain brain and peripheral cells express a truncated POMC mRNA without coding for a signal sequence (Farooqui et al, 1995;Millington et al, 1999).…”

Section: B Proopiomelanocortin Gene Expressionmentioning

confidence: 99%

Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation

et al. 2004

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Section: B Proopiomelanocortin Gene Expressionmentioning

confidence: 99%

Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation

et al. 2004

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“…Interestingly, cell studies suggested a coexpression of MOR/DOR heterodimers with altered ligand affinity and intracellular signaling mechanisms [8]. Furthermore, cardiomyocytes express the opioid precursor peptides proenkephalin [9], dynorphin [10] and POMC-derived mRNA transcripts [11] and they may have autocrine, paracrine and/or endocrine function [12]. Finally, KOR and DOR as well as PDYN and PENK were upregulated in response to congestive heart failure induced by volume overload [6,7].…”

Section: Introductionmentioning

confidence: 99%

Evidence for MOR on cell membrane, sarcoplasmatic reticulum and mitochondria in left ventricular myocardium in rats

et al. 2015

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Cardiac function is one important determinant to maintain tissue oxygenation and is thus highly regulated. In this context, it is interesting that centrally mediated opioidergic influence on cardiac function has long been known. Only recently, KOR and DOR have been found to be expressed in healthy left ventricular myocardium in rats and colocalized with parts of the excitation-contraction-coupling system. However, several comments in literature exist doubting the existence of MOR in cardiac tissue. We, therefore, aimed to detect MOR in rat left ventricular cardiomyocytes, and to evaluate whether MOR and POMC are regulated during heart failure. After IRB approval, heart failure was induced using a modified infrarenal aortocaval fistula (ACF) in male Wistar rats. All rats of the control and ACF group were characterized by their morphometrics and hemodynamics and the existence of MOR and POMC was investigated by means of radioligand binding, double immunofluorescence confocal analysis, RT-PCR and Western blot. Membrane MOR selective binding sites were detected in the left ventricular myocardium, however, they were lower in abundance than KOR- and DOR-specific binding sites and B max of MOR could not be determined. In left ventricular cardiomyocytes, MOR colocalized with parts of the excitation-coupling mechanism, e.g., Cav1.2 of the cell membrane and invaginated T-tubules as well as the ryanodine receptor of the sarcoplasmatic reticulum. More importantly, MOR strongly colocalized with mitochondria of left ventricular cardiomyocytes. Volume overload was not associated with an altered expression of MOR and POMC on both mRNA and protein level. These findings provide evidence for the existence of MOR on the cell membrane, sarcoplasmatic reticulum and mitochondria in left ventricular cardiomyocytes in rats. However, heart failure does not result in an altered expression of the cardiac MOR-opioid system. Thus, MOR agonist treatment-commonly used in the clinical setting-might directly affect cardiac function, which needs to be evaluated in greater detail in the near future.

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“…Intriguingly, melanocyte‐like cells are present in mouse and human hearts that are predisposed to AF 15. Additionally, melanocortin can exert electrophysiological effects and stimulate the expression of interleukins 16. A recent review demonstrated that subclinical hyperthyroidism is associated with increased coronary heart disease events, mortality, and the incidence of AF 17, 18.…”

Section: Discussionmentioning

confidence: 99%

Plasma Circular RNAs, Hsa_circRNA_025016, Predict Postoperative Atrial Fibrillation After Isolated Off‐Pump Coronary Artery Bypass Grafting

Zhang

Shu

et al. 2018

JAHA

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BackgroundCircular RNAs (circRNAs) are pervasively expressed in highly divergent eukaryotes and are stable in body fluids. However, the link between circRNAs and onset of atrial fibrillation (AF) has not previously been investigated. We aimed to identify plasma circRNAs that are able predict AF after cardiac surgery.Methods and ResultsPlasma circRNA expression profiles were investigated in a total of 769 patients with or without AF after isolated off‐pump coronary artery bypass grafting. First, a circRNA microarray was used to screen 13 617 human circRNAs in plasma samples from patients in the discovery cohort (n=30). A quantitative polymerase chain reaction assay was then applied to evaluate the expression of 9 selected circRNAs in the training cohort (n=365). This approach revealed that hsa_circRNA_025016 was upregulated in patients with new‐onset AF with a high diagnostic accuracy as assessed by the area under the receiver operating characteristic curve (=0.802). Additionally, a satisfactory diagnostic performance of hsa_circRNA_025016 was found in the validation cohort (n=284). Furthermore, Kyoto Encyclopedia of Genes and Genomes biological pathway analysis indicated that hsa_circ_025016 participated in melanogenesis, insulin secretion, and the thyroid hormone signaling pathway. A positive correlation between hsa_circ_025016 and fasting blood glucose was also identified in both cohorts.ConclusionsHsa_circ_025016 is a potential biomarker for predicting new‐onset AF after isolated off‐pump coronary artery bypass grafting.

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Localization of pro-opiomelanocortin mRNA transcripts and peptide immunoreactivity in rat heart

Abstract: These results support the hypothesis that cardiomyocytes synthesize POMC peptides.

Cited by 39 publications

References 56 publications

Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation

Targeting Melanocortin Receptors as a Novel Strategy to Control Inflammation

Evidence for MOR on cell membrane, sarcoplasmatic reticulum and mitochondria in left ventricular myocardium in rats

Plasma Circular RNAs, Hsa_circRNA_025016, Predict Postoperative Atrial Fibrillation After Isolated Off‐Pump Coronary Artery Bypass Grafting

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