Localization of Prostaglandin E Receptor Subtypes in the Ciliary Body of Mouse Eye

Takamatsu, Michiya; Hotehama, Y; Goh, Yasumasa; Mishima, Hiromu K.

doi:10.1006/exer.1999.0829

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…On the other hand, high Msx1 expression was also detected in Rathke's pouch in the mouse (MacKenzie et al, 1991; our observations), closely preceding expression of the glycoprotein hormone alpha-subunit gene, which is the first hormone gene to be detected at E11 in the rat (Simmons et al, 1990). In addition, Msx1 is also expressed in other noncerebral structures of high secretory activity, such as the ciliary bodies of the eyes (Monaghan et al, 1991;Civan et al, 1996;Mirshahi et al, 1999;Takamatsu et al, 2000; this study), the uterus (Lyons et al, 1992;Pavlova et al, 1994), and the mammary gland (Phippard et al, 1996). Accordingly, it has been proposed that Msx1 and also Msx2 are linked to exocrine secretions by this gland (Friedmann and Daniel, 1996).…”

Section: Msx1 Is Expressed In the Circumventricular Organssupporting

confidence: 62%

Msx1 disruption leads to diencephalon defects and hydrocephalus

Ramos¹,

Fernández-Llébrez²,

Bach³

et al. 2004

Developmental Dynamics

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We have analyzed the expression of the Msx1 gene in the developing mouse brain and examined the brain phenotype in homozygotes. Msx1 is expressed in every cerebral vesicle throughout development, particularly in neuroepithelia, such as those of the fimbria and the medulla. Timing analysis suggests that Msx1 nLacZ cells delaminate and migrate radially from these epithelia, mainly at embryonic days 14 -16, while immunohistochemistry studies reveal that some of the ␤-galactosidase migrating cells are oligodendrocytes or astrocytes. Our results suggest that the Msx1 neuroepithelia of fimbria and medulla may be a source of glial precursors. The Msx1 mutants display severe hydrocephalus at birth, while the subcommissural organ, the habenula, and the posterior commissure fail to develop correctly. No label was detected in the mutant subcommissural organ using a specific antibody against Reissner's fiber. Besides, the fasciculus retroflexus deviates close to the subcommissural organ, while the paraventricular thalamic nucleus shows histological disorganization. Our results implicate the Msx1 gene in the differentiation of the subcommissural organ cells and posterior commissure and that Msx1 protein may play a role in the pathfinding and bundling of the fasciculus retroflexus and in the structural arrangement of the paraventricular thalamic nucleus.

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Section: Msx1 Is Expressed In the Circumventricular Organssupporting

confidence: 62%

Msx1 disruption leads to diencephalon defects and hydrocephalus

Ramos¹,

Fernández-Llébrez²,

Bach³

et al. 2004

Developmental Dynamics

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“…Currently, four EP receptor subtypes have been identified in smooth muscle cells, two types mediating contraction (EP 1 and EP 3 ), and two types mediating relaxation (EP 2 and EP 4 ), which suggests that the distribution of receptors in a tissue can mediate antagonistic effects of PGE 2 . This pattern of receptor distribution and the effect of PGE on smooth muscle tone have been confirmed in the ciliary muscle from several species including humans (Chen & Woodward 1992;Takamatsu et al 2000;Schlotzer-Schrehardt et al 2002) and from bovine trabecular cells (Krauss et al 1997a,b). Additionally, EP 4 receptor stimulation has been found to increase the trabecular outflow facility in monkeys (Woodward et al 2009).…”

Section: Discussionmentioning

confidence: 75%

Dual effect of prostaglandins on isolated intraocular porcine ciliary arteries

Kringelholt

Simonsen

Bek

2012

Acta Ophthalmologica

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ABSTRACT.Purpose: Prostaglandin analogues are used to reduce the intraocular pressure (IOP) in glaucoma, but also affect the tone of the arteries supplying the ciliary body. Previously, the effect of prostaglandins has been studied on the extraocular ciliary arteries, whereas the effect on intraocular ciliary arteries has not been studied in detail. Methods: Intraocular long posterior porcine ciliary arteries were isolated and mounted in a myograph system for isometric tension recording, and the effects of PGF 2a , the prostaglandin analogue latanoprost, PGD 2 , PGE 2 , PGI 2 and the thromboxane analogue U46619 were studied in the presence and absence of selective receptor antagonists. Results: The prostaglandins PGD 2 and PGE 2 induced relaxation at low concentrations (10 )9 -3 · 10 )7 m), which could be inhibited by blocking either the DP or the EP 4 receptor, whereas PGD 2 , PGE 2 , PGF 2a , latanoprost and U46619 induced contraction at high concentrations (10 )6 -10 )5 m), which could be inhibited by blocking the TP receptor. Additionally, blocking of the FP receptor induced a right-shift of latanoprost-induced contraction. Conclusions: Prostaglandins with affinity to DP 1 and EP 4 receptors induce relaxation at low concentrations, and prostaglandins with affinity to TP and FP receptors induce contraction at high concentrations of intraocular porcine ciliary vessels in vitro. The findings may contribute to understanding the regulation of blood flow to the ciliary body.

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“…The expression of prostanoid EP receptors in the eyes of various animals and humans have been studied. 28,[35][36][37][38][39] In mice, the expression of prostanoid EP1 (cornea, conjunctiva, trabecular meshwork, nonpigmented epithelium of ciliary body, and retina), EP2 (cornea, conjunctiva, trabecular meshwork, iris, ciliary body, and retina), EP3 (nonpigmented epithelium of ciliary body), and EP4 (cornea, conjunctiva, trabecular meshwork, nonpigmented epithelium of ciliary body, and retina) are similar to the distribution of EP receptors in humans. 28 EP1-EP4 receptors are expressed in cornea, conjunctiva, trabecular meshwork, iris, ciliary body, and retina in human eyes.…”

Section: Discussionmentioning

confidence: 99%