Localization of prostaglandin F2  inhibition of lipoprotein use by bovine luteal cells

Grusenmeyer, D. P.; Pate, Joy L.

doi:10.1530/jrf.0.0940311

Cited by 20 publications

(12 citation statements)

References 22 publications

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“…Instead, an experiment was performed in which exogenous progesterone was added to determine the effect of increasing concentrations of progesterone on luteal cell-stimulated T lymphocyte proliferation. In the presence of aminoglutethimide (an inhibitor of the cytochrome P450 side-chain cleavage enzyme) at a concentration previously shown to inhibit lipoproteinstimulated progesterone production by bovine luteal cells [14], luteal cell-stimulated T lymphocyte proliferation was greater compared with cocultures in the absence of aminoglutethimide. This suggests that progesterone synthesis by luteal cells affects T lymphocyte proliferation in this coculture system.…”

Section: Discussionmentioning

confidence: 98%

“…Cocultures of luteal cells and T lymphocytes were carried out in the absence or presence of 50 g/ml of aminoglutethimide, an inhibitor of the cytochrome P450 side-chain cleavage enzyme. This concentration of aminoglutethimide has previously been shown to inhibit progesterone synthesis by cultured bovine luteal cells [14]. Additionally, increasing concentrations of exogenous progesterone were added to cocultures in the presence of aminoglutethimide.…”

Section: Coculture Of Luteal Cells and T Lymphocytesmentioning

confidence: 99%

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Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Cannon

Petroff

Pate

2003

Biology of Reproduction

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Bovine luteal cells express class I and II major histocompatibility complex molecules and stimulate T lymphocyte proliferation in vitro. Proliferation of T lymphocytes is greater in cocultures of luteal cells and T lymphocytes collected following administration of a luteolytic dose of prostaglandin (PG) F2alpha to the cow. Whether this results from changes in luteal cells that increase their ability to stimulate T lymphocyte proliferation or from changes in T lymphocytes that enhance their ability to respond to luteal cells is unclear. To determine which is the case, luteal cell-T lymphocyte cocultures were performed using luteal cells and T lymphocytes isolated from the same animals before and 8 h after administration of PGF2alpha. In the presence of T lymphocytes collected before PGF2alpha administration, luteal cells isolated after PGF2alpha were more potent stimulators of T lymphocyte proliferation than were luteal cells collected before PGF2alpha (P<0.05). The effect of progesterone on luteal cell-stimulated T lymphocyte proliferation was also evaluated. Proliferation of T lymphocytes was greater (P<0.05) in cultures containing the cytochrome P450 side-chain cleavage enzyme-inhibitor aminoglutethimide. Exogenous progesterone caused a dose-dependent inhibition of luteal cell-stimulated T lymphocyte proliferation (P<0.05). Progesterone-receptor mRNA was undetectable in peripheral blood mononuclear cells collected before and after PGF2alpha administration, indicating that the effect of progesterone was not mediated via progesterone receptors in lymphocytes. These results imply that specific changes in luteal cells in response to PGF2alpha enhance the ability of these cells to stimulate T lymphocyte proliferation. These results also demonstrate that progesterone can suppress luteal cell-stimulated T lymphocyte proliferation.

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Section: Discussionmentioning

confidence: 98%

Section: Coculture Of Luteal Cells and T Lymphocytesmentioning

confidence: 99%

Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Cannon

Petroff

Pate

2003

Biology of Reproduction

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“…Treatment with PGF 2␣ inhibited the expression of mRNA for StAR protein in corpora lutea of ewes and cows [5,55]. Others have shown that treatment with PGF 2␣ does not affect the activity, concentration, or mRNA level of P450 scc [56][57][58], or the concentrations of 3␤-HSD, but decreases expression of 3␤-HSD mRNA [59]. In the present study, expression of mRNAs for StAR and 3␤-HSD were decreased at 10, 24, and 48 h when luteal regression was initiated on Day 10, but only at 48 h when treatment was initiated on Day 4.…”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Sayre

Taft

Inskeep

et al. 2000

Biology of Reproduction

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Three experiments were conducted to examine gene expression during induced luteal regression in the cow; the initial purpose was the identification of potential embryotoxins. In experiment 1, changes in gene expression in the corpus luteum (CL) were identified by differential display reverse transcription-polymerase chain reaction (DD-PCR) during the first 72 h of luteal regression in cows treated with prostaglandin F(2alpha) (PGF(2alpha)) on Days 4-7 after estrus. Expression of insulin-like growth factor-binding protein-1 (IGFBP-1) was up-regulated, with greatest expression at 24 h (P < 0.05) after treatment with PGF(2alpha) began. In experiment. 2, IGFBP-1 and its mRNA were quantified in CL collected 24 or 48 h after treatment with PGF(2alpha) on Day 4 or 10 after estrus. Because local mechanisms for exchange of hormones between the ovary and uterus are known in ruminants, uterine flushings were assayed for IGFBP-1 to seek evidence of local transfer of luteal IGFBP-1 to the uterus. IGFBP-1 mRNA was increased (P < 0.05) in CL 24 h after treatment when PGF(2alpha) that began on Day 10, and by 48 h after treatment that began on Day 4. Concentrations of IGFBP-1 increased (P < 0.05) in a pattern similar to mRNA, by 24 h on Day 10, and by 48 h on Day 4. Concentrations of IGFBP-1 in uterine flushings did not change on either day. Concentrations of progesterone decreased (P < 0.05) by 8 h after treatment with PGF(2alpha) that began on Day 10, but not until 24 h after treatment that began on Day 4. In experiment 3, cows received either saline or PGF(2alpha) and CL were collected 2 or 10 h after a single treatment, or 2 h after a second treatment that was given 8 h after the first. Expression of IGFBP-1 was increased by 2 h after treatment with PGF(2alpha) on both Days 4 and 10 after estrus. In conclusion, secretion of IGFBP-1 is increased during luteolysis, and may inhibit the steroidogenic effects of insulin-like growth factor-I (IGF-I), but no evidence was found to implicate IGFBP-1 in the embryotoxic effect of regressing CL.

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“…In contrast, although several in vitro studies have been repeatedly shown that PGF2α directly stimulated basal P secretion/production of bovine midcycle CL as well as early CL [19][20][21], it has been also clearly demonstrated that PGF2α inhibits the conversion of cholesterol to P in luteal cells from midcycle CL [3]. However, PGF2α neither prevents the entry of LIPs into the cells [3] nor has an influence on cellular and mitochondrial cholesterol content [22]. These data suggest that PGF2α exerts its luteolytic effect as a site after cholesterol transport to the cell, but before cholesterol sidechain cleavage in midcycle CL.…”

Section: Discussionmentioning

confidence: 92%

Prostaglandin F2.ALPHA. Increases Lipoprotein Utilization for Progesterone in Bovine Early Corpora Lutea In Vitro.

Kobayashi

Miyamoto

2000

Journal of Reproduction and Development

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Abstract. Luteal cells utilize lipoproteins (LIPs) for progesterone (P) synthesis. A luteolysin prostaglandin F2α (PGF2α) has shown to prevent the LIPs utilization for steroidogenesis in bovine midcycle luteal cells. However, a luteolytic injection of PGF2α before Day 5 (Day 0=estrus) can not induce the regression of the corpus luteum (CL). This suggests that the intraluteal mechanism of PGF2α action on the LIPs utilization in early CL is different from that of the mid luteal stage. Thus, this study aimed to investigate the interaction between PGF2α and LIPs on the secretion of P and oxytocin (OT) from bovine early CL. An in vitro microdialysis system (MDS) was used as a model. Each bovine early CL (Days 3-4) was implanted with the MDS, and maintained in an organ culture chamber. The infusion of LIPs (1 µg/ml) stimulated P release during infusion, but had no effect on OT release. The infusion of PGF2α (10 -5 M) stimulated both P and OT release during infusion. When LIPs were infused after PGF2α exposure, P and OT release continued to be stimulated until 3 h thereafter. The results show that PGF2α is capable of enhancing the LIPs utilization in early CL, thus support the concept that luteal PGF2α acts as a luteotropic promoter in bovine early CL. Key words: PGF2α, Lipoproteins, Early CL, Cow.(J. Reprod. Dev. 46: [335][336][337][338][339] 2000) t is universally known that an injection of prostaglandin F 2α (PGF 2α ) or its analogue after the mid luteal phase of the estrous cycle induces a rapid regression of the corpus luteum (CL) [1]. Bovine luteal cells utilize both low-density lipoprotein (LDL) and high-density lipoprotein (HDL) as substrates of progesterone (P) in vitro [2]. PGF 2α has shown to prevent the utilization of these lipoproteins (LIPs) for steroidogenesis in bovine luteal cells derived from mid cycle CL in vitro [3]. However, an injection of PGF2α before Day 5 (Day 0 = estrus) is not able to induce normal regression of CL [4,5]. The characteristics of PGF 2α receptors in bovine CL do not change from early to mid luteal phase [6]. A single injection of PGF2α on Day 4 of the estrous cycle had no effect on intraluteal and s e r u m P c o n c e n t r a t i o n s a n d s t e a d y -s t a t e concentrations of mRNA encoding steroidogenic acute regulatory protein that is involved in cholesterol utilization in the cow, whereas a treatment of PGF2α on Day 10 decreased these factors [7]. Furthermore, it is known that PGF2α production in early CL is more active than other luteal stages [8,9]. Therefore, the action of PGF2α in early CL on the utilization of LIPs may differ from that of midcycle CL. Thus, in this study, we aimed to investigate the interaction between PGF2α and LIPs on the secretion of P and OT from bovine early CL.

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Localization of prostaglandin F2 inhibition of lipoprotein use by bovine luteal cells

Cited by 20 publications

References 22 publications

Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation1

Increased Expression of Insulin-Like Growth Factor Binding Protein-1 During Induced Regression of Bovine Corpora Lutea1

Prostaglandin F2.ALPHA. Increases Lipoprotein Utilization for Progesterone in Bovine Early Corpora Lutea In Vitro.

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