Localization of soluble major histocompatibility class II-peptide complexes on T cell surface

Arimilli, Subhashini; Deshpande, Shrikant; Nag, Bishwajit

doi:10.1002/1097-0029(20000901)50:5<419::aid-jemt11>3.0.co;2-5

Cited by 1 publication

(1 citation statement)

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“…Colloidal gold has been used as an excellent marker for studies in cell biology (Manara et al, 1990;Stoffel and Friess, 2002), and its high electron-density makes it useful for immunoelectron microscopy applications (Namork, 1989;Arimilli et al, 2000). This study clearly showed a significantly higher density of gold particles on cells with dendritic morphology (dendritic cells), compared with cells with oval morphology (macrophages).…”

Section: Discussionmentioning

confidence: 64%

Antigen-presenting Cells in Human Radicular Granulomas

et al. 2008

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Substantial numbers of dendritic cells have been detected in radicular granulomas. To test the hypothesis that local antigen presentation from dendritic cells to T-cells is involved critically in immunological responses within radicular granulomas, we compared characteristics of dendritic cells and macrophages by morphological and biological analyses. Under light microscopy, HLA-DR+ and CD68+ cells showed diverse profiles, including dendritic-shaped cells. Transmission electron microscopy revealed that HLA-DR+ dendritic cells, with long cytoplasmic processes and lacking distinct phagosomes, were concentrated in the lymphocyte-rich area. HLA-DR alpha-chain, CD83, and CD86 mRNAs from HLA-DR+ dendritic cells, and CD28 mRNA from CD28+ T-cells were up-regulated in lymphocyte-rich area. Scanning electron microscopy demonstrated that the density of gold particles on dendritic cells was higher than that on HLA-DR+ macrophages. These results suggest that dendritic cells in radicular granulomas are associated with local defense reactions as stronger antigen-presenting cells, as compared with macrophages.

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