Localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia

Albrecht, Gerd; Mustroph, Angelika

doi:10.1007/s00425-003-0995-6

Cited by 109 publications

(69 citation statements)

References 40 publications

(36 reference statements)

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“…Unrooted phylogenetic tree of plant Sus proteins constructed using the neighbor-joining method with MEGA 5.0 program. Isozymes and corresponding plant species are: cotton, GaSus1 to 7 (this study); Potato, StSus1 to 4 [9]; Pea, PeaSus1 to 3 [13]; Wheat, TaSus1 and TaSus2 [15]; Arabidopsis thaliana , AtSus1 to 6 [23]; Rice, OsSus1 to 6 [24]; Lotus japonicus , LjSus1 to 6 [25]; Citrus, CitSus1, CitSus2 and CitSusA [29]; Bamboo, BoSus1 to 4 [37]; Tomato, LeSus1 and LeSus2 [38]; Carrot, DcSus1 and DcSus2 [39]; Maize, ZmSh1, ZmSus1 and ZmSus3 [40]; Barley, HvSus1 and HvSus2 [41]; Sugarbeet, SbSS1 [42]; Sorghum, SbSus2 (accession no. FJ513325); Sugarcane, SoSus2 (accession no.…”

Section: Resultsmentioning

confidence: 99%

Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns

Chen

et al. 2012

BMC Plant Biol

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BackgroundIn plants, sucrose synthase (Sus) is widely considered as a key enzyme involved in sucrose metabolism. Several paralogous genes encoding different isozymes of Sus have been identified and characterized in multiple plant genomes, while limited information of Sus genes is available to date for cotton.ResultsHere, we report the molecular cloning, structural organization, phylogenetic evolution and expression profiles of seven Sus genes (GaSus1 to 7) identified from diploid fiber cotton (Gossypium arboreum). Comparisons between cDNA and genomic sequences revealed that the cotton GaSus genes were interrupted by multiple introns. Comparative screening of introns in homologous genes demonstrated that the number and position of Sus introns are highly conserved among Sus genes in cotton and other more distantly related plant species. Phylogenetic analysis showed that GaSus1, GaSus2, GaSus3, GaSus4 and GaSus5 could be clustered together into a dicot Sus group, while GaSus6 and GaSus7 were separated evenly into other two groups, with members from both dicot and monocot species. Expression profiles analyses of the seven Sus genes indicated that except GaSus2, of which the transcripts was undetectable in all tissues examined, and GaSus7, which was only expressed in stem and petal, the other five paralogues were differentially expressed in a wide ranges of tissues, and showed development-dependent expression profiles in cotton fiber cells.ConclusionsThis is a comprehensive study of the Sus gene family in cotton plant. The results presented in this work provide new insights into the evolutionary conservation and sub-functional divergence of the cotton Sus gene family in response to cotton fiber growth and development.

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Section: Resultsmentioning

confidence: 99%

Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns

Chen

et al. 2012

BMC Plant Biol

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“…Indeed, sucrose synthase was among the highly expressed genes in poplar tension wood which is cellulose enriched, suggesting sucrose synthase involvement in stress response [51]. Anoxic rice plants [52] and hypoxic wheat roots [53] showed a high level of sucrose synthase activity and abundant cellulose deposition in the secondary cell walls of wheat, which was postulated to be important for increasing mechanical stability of roots under hypoxia. When the amount of sucrose synthase was reduced in roots of transgenic potatoes, roots were rapidly damaged by hypoxia and showed slow recovery when plants were returned to aerobic conditions [54].…”

Section: Resultsmentioning

confidence: 99%

Apoplast proteome reveals that extracellular matrix contributes to multistress response in poplar

et al. 2010

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BackgroundRiverine ecosystems, highly sensitive to climate change and human activities, are characterized by rapid environmental change to fluctuating water levels and siltation, causing stress on their biological components. We have little understanding of mechanisms by which riverine plant species have developed adaptive strategies to cope with stress in dynamic environments while maintaining growth and development.ResultsWe report that poplar (Populus spp.) has evolved a systems level "stress proteome" in the leaf-stem-root apoplast continuum to counter biotic and abiotic factors. To obtain apoplast proteins from P. deltoides, we developed pressure-chamber and water-displacement methods for leaves and stems, respectively. Analyses of 303 proteins and corresponding transcripts coupled with controlled experiments and bioinformatics demonstrate that poplar depends on constitutive and inducible factors to deal with water, pathogen, and oxidative stress. However, each apoplast possessed a unique set of proteins, indicating that response to stress is partly compartmentalized. Apoplast proteins that are involved in glycolysis, fermentation, and catabolism of sucrose and starch appear to enable poplar to grow normally under water stress. Pathogenesis-related proteins mediating water and pathogen stress in apoplast were particularly abundant and effective in suppressing growth of the most prevalent poplar pathogen Melampsora. Unexpectedly, we found diverse peroxidases that appear to be involved in stress-induced cell wall modification in apoplast, particularly during the growing season. Poplar developed a robust antioxidative system to buffer oxidation in stem apoplast.ConclusionThese findings suggest that multistress response in the apoplast constitutes an important adaptive trait for poplar to inhabit dynamic environments and is also a potential mechanism in other riverine plant species.

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“…Additionally, sucrose molecules regulate gene expression at transcriptional and post-transcriptional levels [81, 82]. Increasing evidence indicates that SUS is essential for cell wall thickening and cotton fiber cell development [83–85]. Sus is preferentially expressed in elongating fiber cells, and antisense suppression of Sus expression reduces hexose levels, leading to a fiberless phenotype [86, 87].…”

Section: Discussionmentioning

confidence: 99%

Quantitative phosphoproteomic profiling of fiber differentiation and initiation in a fiberless mutant of cotton

Pei

et al. 2014

BMC Genomics

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BackgroundThe cotton (Gossypium spp.) fiber cell is an important unicellular model for studying cell differentiation. There is evidence suggesting that phosphorylation is a critical post-translational modification involved in regulation of a wide range of cell activities. Nevertheless, the sites of phosphorylation in G. hirsutum and their regulatory roles in fiber cell initiation are largely unknown. In this study, we employed a mass spectrometry-based phosphoproteomics to conduct a global and site-specific phosphoproteome profiling between ovules of a fuzzless-lintless (fl) Upland cotton (G. hirsutum) mutant and its isogenic parental wild type (WT) at -3 and 0 days post-anthesis (DPA).ResultsA total of 830 phosphopeptides and 1,592 phosphorylation sites from 619 phosphoproteins were identified by iTRAQ (isobaric tags for relative and absolute quantitation). Of these, 76 phosphoproteins and 1,100 phosphorylation sites were identified for the first time after searching the P3DB public database using the BLAST program. Among the detected phosphopeptides, 69 were differentially expressed between the fl mutant and its WT in ovules at -3 and 0 DPA. An analysis using the Motif-X program uncovered 19 phosphorylation motifs, 8 of which were unique to cotton. A further metabolic pathway analysis revealed that the differentially phosphorylated proteins were involved in signal transduction, protein modification, carbohydrate metabolic processes, and cell cycle and cell proliferation.ConclusionsOur phosphoproteomics-based research provides the first global overview of phosphorylation during cotton fiber initiation, and also offers a helpful dataset for elucidation of signaling networks in fiber development of G. hirsutum.Electronic supplementary materialThe online version of this article (doi: 10.1186/1471-2164-15-466) contains supplementary material, which is available to authorized users.

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Localization of sucrose synthase in wheat roots: increased in situ activity of sucrose synthase correlates with cell wall thickening by cellulose deposition under hypoxia

Cited by 109 publications

References 40 publications

Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns

Analyses of the sucrose synthase gene family in cotton: structure, phylogeny and expression patterns

Apoplast proteome reveals that extracellular matrix contributes to multistress response in poplar

Quantitative phosphoproteomic profiling of fiber differentiation and initiation in a fiberless mutant of cotton

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