Localization of Synaptotagmin in the Regenerating Sprouts Emanating from the Nodes of Ranvier.

Uehara, Kaori; Mizoguchi, Akira; Mizuno, Kosaku; Idé, Chizuka

doi:10.1267/ahc.28.401

Cited by 4 publications

(1 citation statement)

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“…We have also demonstrated that synaptotagmin is localized in the regenerating sprouts emanating from the nodes of Ranvier (Uehara et al 1995). The distribution patterns of synaptophysin are almost the same at light and electron microscopic levels with those of synapsin I of the present study.…”

Section: Localization Of Synapsin I In Regenerating Axonssupporting

confidence: 83%

Localization of synapsin I in normal fibers and regenerating axonal sprouts of the rat sciatic nerve

Akagi

Mizoguchi

Sobue

et al. 1996

Histochem Cell Biol

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The localization of synapsin I, a synaptic vesicle-associated protein, was investigated immunocyto-chemically in normal nerve fibers and regenerating axonal sprouts following crush-injuries to the rat sciatic nerve. In normal myelinated axons, weak synapsin I immunoreactivity was found in the axoplasmic/smooth endoplasmic domains, but not in the cytoskeletal domains comprising neurofilaments and microtubules. In non-myelinated axons without dense cytoskeletal structures, moderate immunoreactivity was distributed diffusely throughout the axoplasm. In the crush-injured nerves, intense synapsin I immunoreactivity was demonstrated by light microscopy in early regenerating sprouts emerging from nodes of Ranvier. These nodal sprouts subsequently elongated as regenerating axons through the space between the basal lamina and the myelin sheath (or Schwann cell plasma membrane). Intense synapsin I immunoreactivity was also found in the growth cones of such long regenerating axons. Electron microscopy revealed that synapsin I immunoreactivity was associated mainly with vesicular organelles in the nodal sprouts and growth cones of regenerating axons. Long regenerating axons exhibited no synapsin I immunoreactivity in the shaft, which contained an abundance of neurofilaments. However, vesicle accumulations remaining in the periphery of the shaft still exhibited intense synapsin I immunoreactivity. Thus, it can be concluded that synapsin I is localized at especially high density in the domains comprising vesicular organelles, which are characteristic of early nodal sprouts, as well as in growth cones of regenerating axons. These findings, together with the proposed functions of synapsin I investigated in other studies, suggest that synapsin I may play important roles in vesicular dynamics including the translocation of vesicles to the plasma membrane in sprouts and growth cones of regenerating axons.

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Section: Localization Of Synapsin I In Regenerating Axonssupporting

confidence: 83%