Localization of TFF3 peptide to porcine conjunctival goblet cells

TFF3 is a member of the trefoil factor family (TFF) peptides, which enhance the surface integrity of mucous epithelia, and is typically secreted by intestinal goblet cells together with the mucin MUC2. Reports of the expression of TFF3 within the normal human stomach are contradictory and the precise localisation of TFF3 is unknown. We have mapped the human gastric mucosa by reverse transcription/polymerase chain reaction and Western blot analysis and by immunohistochemistry. Small amounts of TFF3 were detectable in the oxyntic mucosa of the corpus, whereas TFF3 synthesis increased sharply distal to the corpus-antrum transitional zone. TFF3 secretion was demonstrated in the antrum, the pyloric region and the proximal duodenum. High TFF1 levels were present in all regions of the gastric mucosa including the corpus, whereas the proximal duodenum was nearly devoid of TFF1. Immunohistochemistry localised TFF3 to antral and pyloric surface mucous cells. In the antrum, an increasing concentration gradient was found towards cells deeper in the isthmus. TFF1 was confined to superficial pit cells in the antrum. TFF3 was also present in specific layers of the laminated mucous gel of the antral and pyloric regions and variable concentrations of TFF3 (2-62 nmol/l) were measured in gastric juice. Here, a probably N-terminally shortened variant is present that forms disulphide-linked dimers. Thus, TFF3 is a typical secretory peptide of the normal human antral and pyloric gastric mucosa and has a gastric expression pattern different from that of TFF1 and TFF2. TFF3 might therefore have important physiological functions in the stomach, e.g. as a luminal surveillance peptide maintaining particularly the integrity of the antral and pyloric mucosa.

Section: Tff1 and Tff3 Synthesis At The Antroduodenal Transitional Zonesupporting

confidence: 93%

A gradient of TFF3 (trefoil factor family�3) peptide synthesis within the normal human gastric mucosa

Kouznetsova

Peitz

Vieth

et al. 2004

“…Furthermore, a polyclonal rabbit antiserum against the C-terminus of rat TFF3 (anti-rTFF3-1; Wiede et al 1999;Probst et al 1995) was used for immunohistochemistry. These antisera have also previously been shown to recognize porcine TFF3 (Jagla et al 1999b) because of the high similarity of the C-terminal regions of human, rat and porcine TFF3 (Hauser et al 1993;Probst et al 1995;Wintero et al 1996). OT was localized by using a commercial polyclonal rabbit antiserum (Boehringer Ingelheim, Germany).…”

Section: Antisera and Western Blot Analysismentioning

confidence: 99%

Ultrastructural co-localization of TFF3-peptide and oxytocin in the neural lobe of the porcine pituitary

Schwarz

Jagla

Wiede

et al. 2001

TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of many mucous epithelial cells. TFF3 is also synthesized in oxytocinergic neurons in the paraventricular and supraoptic nuclei of the human hypothalamus. Here, TFF3 and oxytocin are shown to be co-localized within the same secretory vesicles in the neural (posterior) lobe of the procine pituitary by means of immunoelectron microscopy. Relatively large amounts of TFF3, but not TFF1 and TFF2, are present in the neural lobe of the porcine pituitary, where it is probably released into the bloodstream.

“…Samples of human uterine tissue were fixed in HEPES-buffered 4% paraformaldehyde overnight at 4°C, embedded in Techno- Table 1 Oligodeoxynucleotides used for RT-PCR analysis and calculated size of the products vit 7100 (Heraeus Kulzer, Wehrheim, Germany) and processed as described in detail elsewhere (Wiede et al 1999;Jagla et al 1999b) but omitting the papain treatment; the primary antiserum anti-rTFF3-1 was used at a 1:500 dilution. The specifity of the staining was tested by competition with the corresponding synthetic peptide, i.e., 1 ml anti-rTFF3-1 (1:500 dilution) was pre-adsorbed with 4 µg synthetic peptide FKPLQETECTF (representing the C-terminal of rat TFF3) overnight at 37°C and then used for immunofluorescence.…”

Section: General Histology and Immunofluorescencementioning

confidence: 99%

Synthesis and localization of the mucin-associated TFF-peptides in the human uterus

Wiede

Hinz

Canzler

et al. 2001

Self Cite

TFF-peptides (formerly P-domain peptides, trefoil factors) are typical secretory products of mucin-producing cells and are thought to influence the rheological properties of mucous gels. Here, the localization of these peptides in the human uterus was investigated. An analysis of TFF-peptides mRNA by the polymerase chain reaction revealed TFF3 mainly in the endocervix and smaller amounts in the endometrium. TFF1 and TFF2 mRNA was detectable occasionally in the endocervix and very rarely in the endometrium. Western blot analysis detected only TFF3 in tissue extracts of the endocervix and as a constituent of human cervical mucus. Immunofluorescence localized TFF3 in the surface epithelium of the endocervix and in gland-like structures of the cervical epithelium.