Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33→q34

Duncan, Alessandra M.V.; Chow, W.H.A.; Robinson, B.H.

doi:10.1159/000133340

Cited by 13 publications

(6 citation statements)

References 4 publications

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“…We have cloned and confirmed the sequence of the cDNAs encoding these proteins from a human heart library (data not shown). Based on the nomenclature developed by Robinson and colleagues (22,23), the unnamed subunit has been termed NDUFA11, since its bovine counterpart was shown to be a part of the 1␣ subcomplex. Furthermore, it has been postulated that this protein may be involved in protein import into the mitochondrion (11,24).…”

Section: Purification Of the Human Nadh Dehydrogenase Complex I 13620mentioning

confidence: 99%

The Subunit Composition of the Human NADH Dehydrogenase Obtained by Rapid One-step Immunopurification

Murray

Zhang²,

Taylor³

et al. 2003

Journal of Biological Chemistry

100

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Section: Purification Of the Human Nadh Dehydrogenase Complex I 13620mentioning

confidence: 99%

The Subunit Composition of the Human NADH Dehydrogenase Obtained by Rapid One-step Immunopurification

Murray

Zhang²,

Taylor³

et al. 2003

Journal of Biological Chemistry

100

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“…It consists of at least 41 different polypeptide subunits (Walker et al, 1992a), seven of which are encoded by the mitochondrial genome and 34 by the nuclear genome. To date only 14 human nuclear complex I genes have been mapped and/or cloned (Duncan et al, 1992;Spencer et al, 1992;Ali et al, 1993;Baens et al, 1993;de Coo et al, 1995de Coo et al, , 1997Hattori et al, 1995;Hyslop et al, 1996;Gu et al, 1996;Zhuchenko et al, 1996;Russel et al, 1997;Procaccio et al, 1997;Dunbar et al, 1997). Dysfunction of the ETC is believed to contribute to many diseases including encephalomyopathies, diabetes, blindness, deafness, and neurodegenerative disorders (Larsson and Clayton, 1995;Parker and Parks, 1995).…”

mentioning

confidence: 99%

lntron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain

Emahazion¹,

Beskow²,

Gyllensten³

et al. 1998

Cytogenet Genome Res

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At least 34 complex I subunits of the electron transport chain are encoded by the nuclear genome, but only 14 of these have been mapped in the human. To rapidly map additional subunits, we have performed a combination of database mining and direct “wet” experimentation to identify intron and/or 5′ upstream genomic DNA regions for 16 complex I genes. Wet experimentation was applied to 5 genes, and involved direct PCR amplification of introns by inter-exon PCR or splinkerette based PCR walking. Database mining was applied to 11 genes, and entailed the identification of incompletely spliced mRNAs and genomic CpG island clone sequences. This data was in files that carried no documentary description of the non-exon regions. Non-exon sequences were thus derived for 15 complex I genes and used to design functional gene specific PCR assays. Radiation hybrid mapping of these PCRs located 15 complex I genes to chromosomes l, 4, 5 (2 genes), 7 (2 genes), 8, 9 (2 genes), 11, 14, 16 (2 genes), 18, and 19.

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“…This repre sents a significant advance over the 7 members of this gene set previously cloned and mapped (Duncan et al, 1992;Spencer et al, 1992;Ali et al. 1993;deCoo et al, 1995;Hattori et al, 22 Cytogcnct Cell Genet 78:21-24 (1997) 1995; Gu et al, 1996;Hyslop et al, 1996: Zhuchenko et al, 1996: Russel et al.…”

Section: Discussionmentioning

confidence: 82%

In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes

Dunbar¹,

Shibasaki

Dobbie³

et al. 1997

Cytogenet Genome Res

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Using human and bovine short cDNA sequences as probes we screened human cosmid and P1 libraries for components of the complex I multi-subunit enzyme of oxidative phosphorylation. We isolated genomic recombinants encoding d-B8 (gene NDUFA2), d-B14 (gene NDUFA6), d-B14.5a (gene NDUFA7), cI-ASHI (gene NDUFB8) and d-23kD (gene NDUFS8). Genomic versions of these genes have not been previously cloned in the human although they are represented as anonymous entries in public cDNA databases. By using the derived genomic clones for in situ hybridisation studies we determined the following chromosome locations: NDUFA2, 5q31; NDUFA6, 21q22; NDUFA7, 20pl3; NDUFB8, 12q21; NDUFS8, 3q28.

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Localization of the human 75-kDal Fe-S protein of NADH-coenzyme Q reductase gene (NDUFS1) to 2q33→q34

Abstract: The gene for the human 75-kDal Fe-S protein of the NADH-coenzyme Q reductase was localized to 2q33→q34 by in situ hybridization to human metaphase chromosomes banded, by BrdU-incorporation, to the 500–550 band level of resolution.

Cited by 13 publications

References 4 publications

The Subunit Composition of the Human NADH Dehydrogenase Obtained by Rapid One-step Immunopurification

The Subunit Composition of the Human NADH Dehydrogenase Obtained by Rapid One-step Immunopurification

lntron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain

In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes

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