Localization of the human cholecystokinin-B/gastrin receptor gene (CCKBR) to chromosome 11p15.5→p15.4 by fluorescence in situ hybridization

Zimonjic, D.B.; Popescu, Nicholas C.; Matsui, Toshimitsu; Ito, Mitsuhiro; Chihara, Kazuo

doi:10.1159/000133628

Cited by 30 publications

(18 citation statements)

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“…A 4.1-kb FRP genomic fragment obtained from a human fibroblast genomic DNA library (Stratagene) was labeled with biotin or digoxigenin and used as a probe for in situ hybridization to locate the FRP gene in chromosomal preparations of methotrexate-synchronized normal peripheral human lymphocyte cultures. The conditions for hybridization, detection of fluorescent signal, digital-image acquisition, processing, and analysis were as described (26). The identity of the chromosomes with specific signal was confirmed by rehybridization using a chromosome 8-specific probe, and the signal was localized on G-banded chromosomes.…”

Section: Methodsmentioning

confidence: 99%

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

Kelley

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

384

306

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Section: Methodsmentioning

confidence: 99%

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

Kelley

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

384

306

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“…Chromosomes were obtained from peripheral lymphocyte cultures after methotrexate-thymidine release treatments and used for fluorescence in situ hybridization (FISH) as described previously (29,54). The probes were labeled by nick translation with biotin-dUTP or digoxigen-11-dUTP.…”

Section: Methodsmentioning

confidence: 99%

NAB2, a Corepressor of NGFI-A (Egr-1) and Krox20, Is Induced by Proliferative and Differentiative Stimuli

Svaren

Sevetson

Apel

et al. 1996

Molecular and Cellular Biology

333

328

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Previous work had identified a corepressor, NAB1, which represses transcriptional activation mediated by NGFI-A (also known as Egr-1, zif268, and Krox24) and Krox20. These zinc finger transcription factors are encoded by immediate-early genes and have been implicated in a wide variety of proliferative and differentiative processes. We have isolated and characterized another corepressor, NAB2, which is highly related to NAB1 within two discrete domains. The first conserved domain of NAB2 mediates an interaction with the R1 domain of NGFI-A. NAB2 represses the activity of both NGFI-A and Krox20, and its expression is regulated by some of the same stimuli that induce NGFI-A expression, including serum stimulation of fibroblasts and nerve growth factor stimulation of PC12 cells. The human NAB2 gene has been localized to chromosome 12q13.3-14.1, a region that is rearranged in several solid tumors, lipomas, uterine leiomyomata, and liposarcomas. Sequencing of the Caenorhabditis elegans genome has identified a gene that bears high homology to both NAB1 and NAB2, suggesting that NAB molecules fulfill an evolutionarily conserved role.Transcriptional control plays a vital role in regulating fundamental cellular processes such as proliferation, differentiation, and cell death. Changes in gene expression are often effected by altering the expression level, activity, and/or nuclear localization of transcription factors that bind to promoter and enhancer regions. In addition, many transcription factors are regulated by direct interactions with other proteins which modulate the level of transcriptional activation.

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“…Lambda clone lMAM1 and subclone pMAM4.3 were labeled with biotin or digoxigenin using a random-prime DNA labeling kit (Boehringer-Mannheim). FISH was performed as perviously described (Zimonjic et al, 1994). Slides were pretreated with RNase, denatured in 26SSC/ 70% formamide for 2 min at 708C, and hybridized with 200 ng of DNA probe in 26SSC/50% formamide/10% dextran sulfate/26Denhart's/1% Tween20 for 18 h at 378C.…”

Section: Chromosomal Mappingmentioning

confidence: 99%

Structure and transcriptional regulation of the human mammaglobin gene, a breast cancer associated member of the uteroglobin gene family localized to Chromosome 11q13

et al. 1998

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The mammaglobin gene encodes a novel secreted protein whose corresponding mRNA is frequently up-regulated in human breast cancer. In non-malignant tissues, expression is also strictly limited to the mammary epithelium. To better understand the mechanisms controlling these patterns of expression, we have isolated the human mammaglobin gene and performed an initial assessment of its promoter activity. Mammaglobin gene architecture is very similar to that of a family of related genes that includes uteroglobin and rat prostatein subunits C1, C2, and C3. However, the mammaglobin gene itself is not well conserved phylogenetically. The human mammaglobin gene is localized by¯uorescent in situ hybridization to chromosome 11 band q13, a genomic region frequently ampli®ed in breast neoplasia. The sequence of proximal 1 kb of mammaglobin promoter contains several potential transcriptional control elements and directs high-level expression of a transfected reporter construct in human breast tumor cell lines. However, comparable levels of reporter gene expression are also seen in non-mammary human cell lines. These data suggest that, unlike related gene family members, the striking breast-speci®c expression and tumor-associated overexpression of mammaglobin is mediated by complex transcriptional control at more distal sequence elements.

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Localization of the human cholecystokinin-B/gastrin receptor gene (CCKBR) to chromosome 11p15.5→p15.4 by fluorescence in situ hybridization

Cited by 30 publications

References 0 publications

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action

NAB2, a Corepressor of NGFI-A (Egr-1) and Krox20, Is Induced by Proliferative and Differentiative Stimuli

Structure and transcriptional regulation of the human mammaglobin gene, a breast cancer associated member of the uteroglobin gene family localized to Chromosome 11q13

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