2007
DOI: 10.1128/jb.01451-06
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Localization of the Naturally Occurring Plasmid ColE1 at the Cell Pole

Abstract: The naturally occurring plasmid ColE1 was found to localize as a cluster in one or both of the cell poles of Escherichia coli. In addition to the polar localization of ColE1 in most cells, movement of the plasmid to the midcell position was observed in time-lapse studies. ColE1 could be displaced from its polar location by the p15A replicon, pBAD33, but not by plasmid RK2. The displacement of ColE1 by pBAD33 resulted in an almost random positioning of ColE1 foci in the cell and also in a loss of segregational … Show more

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Cited by 41 publications
(52 citation statements)
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“…In this study, target RNA was transcribed from a high copy number plasmid pETDuet1 with a ColE1 origin of replication. ColE1-containing plasmids form clusters at the cell poles, which migrate to the mid-and quarter points of the cell during plasmid segregation (31). Appearance of fluorescent focal points at the mid-and quarter-points in some cells (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…In this study, target RNA was transcribed from a high copy number plasmid pETDuet1 with a ColE1 origin of replication. ColE1-containing plasmids form clusters at the cell poles, which migrate to the mid-and quarter points of the cell during plasmid segregation (31). Appearance of fluorescent focal points at the mid-and quarter-points in some cells (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…Considering the sensitivity of the quantitative PCR (qPCR)-based plasmid copy number assay used by Sengupta et al, this might indeed be the case. Other multicopy plasmids, such as RK2 and ColE1, associate into clusters that localize to fixed positions within cells (13,16). For this reason, foci formed from RK2 and ColE1 harbor more than one plasmid.…”
Section: Multiple Plasmid Copies and Diffusionmentioning
confidence: 99%
“…Since the localization of some plasmids, such as P1, F, and R27, is affected if their partitioning proteins are inactivated (6,9,11), the big question is, what is the real advantage of harboring a partitioning system? ColE1 clusters can be disrupted by pBAD33 (a p15A-based plasmid), leading to plasmid loss, but not by RK2 (16). However, ColE1 can be made more stable in the presence of pBAD by incorporating a partitioning system derived from another plasmid, such as F (16).…”
Section: Pairing Clustering and The Roles Of Partitioning Proteinsmentioning
confidence: 99%
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