Localization of the pig luteinizing hormone/choriogonadotropin receptor gene (LHCGR) by radioactive and nonradioactive in situ hybridization

Abstract

The porcine gene for luteinizing hormone/choriogonadotropin receptor (LHCGR) was localized to chromosome 3q2.2→q2.3 using radioactive and nonradioactive in situ hybridization. A computer-assisted image-analysis system was developed which facilitated detection of the position of silver grains and fluorescent spots on the chromosomes after in situ hybridization. Compared with autoradiographic visualization, the nonisotopic procedure proved to be more rapid, precise, and highly specific; however, nonradiographic in situ hybridization was much less efficient than the autoradiographic technique for the detection of unique DNA sequences with small probes. From these results and published gene-mapping data, it was concluded that the synteny between LHCGR and MDH1 observed in man is conserved in the pig genome.

Localization of IGF1R and EDN genes to pig chromosomes 1 and 7 by in situ hybridization

“…Slides were rcstained with Giemsa, and all chromosomes in preselected metaphases that were associat ed with a silver grain were identified. The relative positions of the grains associated with a chromosome were measured by using a computer-assisted image analysis system, as previously described (Yerle et al, 1992). The num ber oflabeled sites was automatically plotted for each chromosome, resulting in a histogram of the grain distribution.…”

Assignment of Stearoyl Coenzyme A Desaturase gene (SCD1) to chicken chromosome R-band 6q14 by in situ hybridization

“…According to Yerle et al (1992). 100 ng of cosrnid 3.1 were biotin labeled by random priming using biotin-16-dUTP (Boehringer Mannheim).…”

A new marker (NGFB) on pig chromosome 4, isolated by using a consensus sequence conserved among species

“…After in situ hybridization, the slides were res tained with Giemsa, and the preselected metaphases were compared with their appearance before hybridization to identify the chromosomes. The rela tive positions of the grains associated with a chromosome were measured by using a computer-assisted image analysis system, as previously described (Yerle et al" 1992). Statistical evaluation of the number of silver grains per unit chromosome length was performed assuming a Poisson distribution of the grains along the length of the chromosome.…”