Localization of the recombination points in a family with two DR/DP recombinations

Thomsen, Mögens; Cullen, Michael; Carrington, Mary; Foissac, Alexandra; Abbal, M.; Préval, C. de; Crouau‐Roy, Brigitte; Cambon‐Thomsen, Anne

doi:10.1111/j.1399-0039.1996.tb02591.x

Cited by 3 publications

(1 citation statement)

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“…A1 though some recombination events have been quite precisely mapped within the MHC (44), the existence and localization of hot spots of recombination, the hypothesis of haplotype-dependent recombinations or the effect of selective pressure are remaining questions (27, 70, 7 1). Microsatellites are very useful in such investigations, for a more precise mapping of recombination points (27,72) and for a precise location of genes or new microsatellites (25,73). Studies on recombinant families using microsatellites have been carried out recently in the framework of the XlIth International Histocompatibility Workshop.…”

Section: Recombination Studies and Mapping Applicationsmentioning

confidence: 99%

Review article Microsatellites in the HLA region: an overview

Foissac¹,

Crouau‐Roy²,

Fauré³

et al. 1997

Tissue Antigens

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Microsatellites are repeats of a DNA base motif (1-6 bp, mostly CA repeats) up to 100 times; they are distributed regularly all over the genome. Many of them are polymorphic and their high polymorphism is based upon a variable number of repeats. They are widely used for genetic mapping, linkage analysis, population genetics, evolutionary studies and in forensic medicine. Such markers have also been described in the HLA region since 1991, and a growing interest in their potential applications is being expressed. The aims of this review are: 1) to outline the presently available information from literature and molecular databases concerning 53 microsatellites in the HLA region (localization, type of repeat, number of alleles, heterozygosity, primers used for amplification); 2) to address the question of technical pitfalls when using such markers; 3) to discuss specific features such as their mutation rate (10 (-3) to 10 (-6), which is higher than that reported for HLA genes, and their linkage disequilibrium with HLA alleles; 4) to present an integrated map of microsatellites and genes of this region; and 5) to provide a synopsis of their different applications in HLA-related fields (disease studies, population genetics, recombination point studies, HLA region mapping, transplantation) along with perspectives for future use. Although some HLA region microsatellites have already been applied to the analysis of more than 10 diseases, it is now evident that their use in population genetics and the determination of genomic compatibility in bone marrow transplantation represent growing areas of application.

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Section: Recombination Studies and Mapping Applicationsmentioning

confidence: 99%